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</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="20%"> | + | <td width="20%">BBa_K2740001</td> |
− | <td width="30%"><em> | + | <td width="30%"><em>CR1 Pnif</em></td> |
− | <td width="50%"></td> | + | <td width="50%">CR1 Pnif is a σ70 (σA)-dependent promoter located upstream of CR1 nifB(BBa_K2740002), which is negatively regulated in response to oxygen and fixed nitrogen.</td> |
</tr> | </tr> | ||
− | + | <tr> | |
− | <td width="20%"> | + | <td width="20%">BBa_K2740002</td> |
− | <td width="30%"><em> | + | <td width="30%"><em>CR1 nifB</em></td> |
− | <td width="50%"></td> | + | <td width="50%">CR1 nifB is essential for nitrogen fixation. If the CR1 nifB was deleted, acetylene reduction have not been detected, which meant the nitrogen fixation not happened.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="20%"> | + | <td width="20%">BBa_K2740003</td> |
− | <td width="30%"><em> | + | <td width="30%"><em>CR1 nifH</em></td> |
− | <td width="50%"></td> | + | <td width="50%">This part codes Fe protein which is an electron donor to the MoFe protein attributing to the electron delivery in the nitrogen fixation system.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="20%"> | + | <td width="20%">BBa_K2740004</td> |
− | <td width="30%"><em> | + | <td width="30%"><em>CR1 nifD</em></td> |
− | <td width="50%"></td> | + | <td width="50%">CR1 nifD codes MoFe protein which is an α2β2 heterotetramer together with CR1 nifK(BBa_K2740004). And the enzyme is responsible for fixing-nitrogen.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="20%"> | + | <td width="20%">BBa_K2740005</td> |
− | <td width="30%"><em> | + | <td width="30%"><em>CR1 nifK</em></td> |
− | <td width="50%"></td> | + | <td width="50%"> CR1 nifK codes MoFe protein which is an α2β2 heterotetramer together with CR1 nifD(BBa_K2740003). And the enzyme encoded by CR1 nifK is essential for fixing-fixation.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="20%"> | + | <td width="20%">BBa_K2740006</td> |
− | <td width="30%"><em> | + | <td width="30%"><em>CR1 nifE</em></td> |
− | <td width="50%"></td> | + | <td width="50%">CR1 nifE contributes to the synthesis and insertion of FeMo-co into nitrogenase together with CR1 nifN. And the FeMo-co in the nitrogenase is quite for maintain its function.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="20%"> | + | <td width="20%">BBa_K2740007</td> |
− | <td width="30%"><em> | + | <td width="30%"><em>CR1 nifN</em></td> |
− | <td width="50%"></td> | + | <td width="50%"> CR1 nifN contributes to the synthesis and insertion of FeMo-co into nitrogenase. </td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="20%"> | + | <td width="20%">BBa_K2740008</td> |
− | <td width="30%"><em> | + | <td width="30%"><em>CR1 nifX</em></td> |
− | <td width="50%"></td> | + | <td width="50%"> CR1 nifX contributes to the synthesis and insertion of FeMo-co into nitrogenase</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="20%"> | + | <td width="20%">BBa_K2740009</td> |
− | <td width="30%"><em> | + | <td width="30%"><em>CR1 HesA</em></td> |
− | <td width="50%"></td> | + | <td width="50%"> CR1 hesA The gene hesA encodes a NAD/FAD-binding protein involved in molybdopterin and thiamine biosynthesis.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="20%"> | + | <td width="20%">BBa_K2740010</td> |
− | <td width="30%"><em> | + | <td width="30%"><em>CR1 nifV</em></td> |
− | <td width="50%"></td> | + | <td width="50%">CR1 nifV contributes to synthesizing the homocitrate moiety of FeMo-co which is essential for the nitrogen fixation.</td> |
</tr> | </tr> | ||
<tr> | <tr> | ||
− | <td width="20%"> | + | <td width="20%">BBa_K2740011</td> |
− | <td width="30%"><em>Tnif</em></td> | + | <td width="30%"><em>CR1 Tnif</em></td> |
− | <td width="50%"></td> | + | <td width="50%">CR1 Tnif is a specific terminator for the whole nif gene cluster.</td> |
</tr> | </tr> | ||
</table> | </table> |
Revision as of 06:43, 19 September 2018
Basic_parts | ||
Part number | Part name | Natural function of part |
BBa_K2740001 | CR1 Pnif | CR1 Pnif is a σ70 (σA)-dependent promoter located upstream of CR1 nifB(BBa_K2740002), which is negatively regulated in response to oxygen and fixed nitrogen. |
BBa_K2740002 | CR1 nifB | CR1 nifB is essential for nitrogen fixation. If the CR1 nifB was deleted, acetylene reduction have not been detected, which meant the nitrogen fixation not happened. |
BBa_K2740003 | CR1 nifH | This part codes Fe protein which is an electron donor to the MoFe protein attributing to the electron delivery in the nitrogen fixation system. |
BBa_K2740004 | CR1 nifD | CR1 nifD codes MoFe protein which is an α2β2 heterotetramer together with CR1 nifK(BBa_K2740004). And the enzyme is responsible for fixing-nitrogen. |
BBa_K2740005 | CR1 nifK | CR1 nifK codes MoFe protein which is an α2β2 heterotetramer together with CR1 nifD(BBa_K2740003). And the enzyme encoded by CR1 nifK is essential for fixing-fixation. |
BBa_K2740006 | CR1 nifE | CR1 nifE contributes to the synthesis and insertion of FeMo-co into nitrogenase together with CR1 nifN. And the FeMo-co in the nitrogenase is quite for maintain its function. |
BBa_K2740007 | CR1 nifN | CR1 nifN contributes to the synthesis and insertion of FeMo-co into nitrogenase. |
BBa_K2740008 | CR1 nifX | CR1 nifX contributes to the synthesis and insertion of FeMo-co into nitrogenase |
BBa_K2740009 | CR1 HesA | CR1 hesA The gene hesA encodes a NAD/FAD-binding protein involved in molybdopterin and thiamine biosynthesis. |
BBa_K2740010 | CR1 nifV | CR1 nifV contributes to synthesizing the homocitrate moiety of FeMo-co which is essential for the nitrogen fixation. |
BBa_K2740011 | CR1 Tnif | CR1 Tnif is a specific terminator for the whole nif gene cluster. |