Difference between revisions of "Team:Goettingen/Part Collection"

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      <h2>Part collection for glyphosate resistance</h2>
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      <p>In this collection, we gathered all parts that contribute to the resistance against the herbicide glyphosate. First of all the two newly identified glyphosate transporters GltT and GltP. If both transporters are not present anymore, the soil-bacterium <em>Bacillus&nbsp;subtilis</em> is glyphosate-resitant. The target of glyphosate is the EPSP-synthase, which is encoded by <em>aroE</em> in <em>Bacillus&nbsp;subtilis</em> and by <em>aroA</em> in <em>Escherichia&nbsp;coli</em>. If the target is mutated,  like in our part <a href="http://parts.igem.org/Part:BBa_K2586020" target="_blank">BBa_K2586020</a>, the cells are characterized by a high glyphosate-resistance. If the cells express the gene for the Glyphosate-N-acetyl-transferase (GAT), they are also resistant.</p>
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      <div class="notebook-table article_table">
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        <table>
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            <tr>
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                <th>Part Number and Name</th>
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                <th class="article-table_right">Short Description</th>
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                <th>Type</th>
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                <th>Length [bp]</th>
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            </tr>
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            <tr>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586001" target="_blank">BBa_K2586001</a>/<i>gltT</i></td>
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                <td>Uptake of glutamate from the environment </td>
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                <td>Coding</td>
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                <td>1290</td>
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            </tr>
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            <tr>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586002" target="_blank">BBa_K2586002</a>/<i>gltP</i></td>
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                <td>Uptake of glutamate from the environment </td>
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                <td>Coding</td>
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                <td>1245</td>
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            </tr>
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            <tr>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586003" target="_blank">BBa_K2586003</a>/<i>aroE</i></td>
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                <td>Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate</td>
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                <td>Coding</td>
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                <td>1287</td>
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            </tr>
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            <tr>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586007" target="_blank">BBa_K2586007</a>/<i>aroA</i></td>
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                <td>Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate</td>
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                <td>Coding</td>
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                <td>1290</td>
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            </tr>
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            <tr>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586019" target="_blank">BBa_K2586019</a>/<i>gat</i></td>
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                <td>Glyphosate N-acetyl-transferase</td>
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                <td>Coding</td>
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                <td>480</td>
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            </tr>
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            <tr>
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                <td><a href="http://parts.igem.org/Part:BBa_K2586020" target="_blank">BBa_K2586020</a>/<i>aroA*</i></td>
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                <td>Mutated <em>aroA</em></td>
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                <td>Coding</td>
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                <td>480</td>
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            </tr>
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        </table>
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    </div>
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    </div>
 
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Revision as of 12:01, 5 October 2018

Part collection for glyphosate resistance

In this collection, we gathered all parts that contribute to the resistance against the herbicide glyphosate. First of all the two newly identified glyphosate transporters GltT and GltP. If both transporters are not present anymore, the soil-bacterium Bacillus subtilis is glyphosate-resitant. The target of glyphosate is the EPSP-synthase, which is encoded by aroE in Bacillus subtilis and by aroA in Escherichia coli. If the target is mutated, like in our part BBa_K2586020, the cells are characterized by a high glyphosate-resistance. If the cells express the gene for the Glyphosate-N-acetyl-transferase (GAT), they are also resistant.

Part Number and Name Short Description Type Length [bp]
BBa_K2586001/gltT Uptake of glutamate from the environment Coding 1290
BBa_K2586002/gltP Uptake of glutamate from the environment Coding 1245
BBa_K2586003/aroE Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate Coding 1287
BBa_K2586007/aroA Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate Coding 1290
BBa_K2586019/gat Glyphosate N-acetyl-transferase Coding 480
BBa_K2586020/aroA* Mutated aroA Coding 480