Difference between revisions of "Team:Goettingen/Part Collection"
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<h2>Part collection for glyphosate resistance</h2> | <h2>Part collection for glyphosate resistance</h2> | ||
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| − | <p>In this collection, we gathered all parts that contribute to the resistance against the herbicide glyphosate. First of all the two newly identified glyphosate transporters GltT and GltP. If both transporters are not present anymore, the soil | + | <p>In this collection, we gathered all parts that contribute to the resistance against the herbicide glyphosate. First of all the two newly identified glyphosate transporters GltT and GltP. If both transporters are not present anymore, the soil bacterium <em>B. subtilis</em> is glyphosate resistant. The target of glyphosate is the EPSP synthase, which is encoded by the <em>aroE</em> gene in <em>B. subtilis</em> and by the <em>aroA</em> gene in <em>E. coli</em>. If the target is mutated, like in our part <a href="http://parts.igem.org/Part:BBa_K2586020" target="_blank">BBa_K2586020</a>, the cells are characterized by a high glyphosate resistance. If the cells express the gene encoding the glyphosate-<i>N</i>-acetyl-transferase (GAT), they are also resistant. In conclusion, this collection provides options to genetically engineer organisms for enhancing the resistance towards glyphosate.</p> |
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<td><a href="http://parts.igem.org/Part:BBa_K2586001" target="_blank">BBa_K2586001</a>/<i>gltT</i></td> | <td><a href="http://parts.igem.org/Part:BBa_K2586001" target="_blank">BBa_K2586001</a>/<i>gltT</i></td> | ||
| − | <td>Uptake of glutamate from the environment </td> | + | <td>Uptake of glutamate or glyphosate from the environment</td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>1290</td> | <td>1290</td> | ||
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<td><a href="http://parts.igem.org/Part:BBa_K2586002" target="_blank">BBa_K2586002</a>/<i>gltP</i></td> | <td><a href="http://parts.igem.org/Part:BBa_K2586002" target="_blank">BBa_K2586002</a>/<i>gltP</i></td> | ||
| − | <td>Uptake of glutamate from the environment </td> | + | <td>Uptake of glutamate or glyphosate from the environment </td> |
<td>Coding</td> | <td>Coding</td> | ||
<td>1245</td> | <td>1245</td> | ||
Revision as of 12:51, 11 October 2018
Team Göttingen
iGEM 2018
Glyphosate on my plate?
| E-mail: | igem2018@gwdg.de |
|---|---|
| Adress: | c/o iGEM 2018 |
| Department of General Microbiology | |
| Grisebachstraße 8 | |
| 37077 Göttingen | |
| Germany |
We are especially grateful to our numerous sponsors on gofundme, who supported our project and made it possible.
Every public donor, who helped us to achieve our goals got a special place on our bacterial wall of fame and a handdrawn microbe to show our appreciation. A link to that wall can be found here:
Part collection for glyphosate resistance
In this collection, we gathered all parts that contribute to the resistance against the herbicide glyphosate. First of all the two newly identified glyphosate transporters GltT and GltP. If both transporters are not present anymore, the soil bacterium B. subtilis is glyphosate resistant. The target of glyphosate is the EPSP synthase, which is encoded by the aroE gene in B. subtilis and by the aroA gene in E. coli. If the target is mutated, like in our part BBa_K2586020, the cells are characterized by a high glyphosate resistance. If the cells express the gene encoding the glyphosate-N-acetyl-transferase (GAT), they are also resistant. In conclusion, this collection provides options to genetically engineer organisms for enhancing the resistance towards glyphosate.
| Part Number and Name | Type | Length [bp] | |
|---|---|---|---|
| BBa_K2586001/gltT | Uptake of glutamate or glyphosate from the environment | Coding | 1290 |
| BBa_K2586002/gltP | Uptake of glutamate or glyphosate from the environment | Coding | 1245 |
| BBa_K2586003/aroE | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate | Coding | 1287 |
| BBa_K2586007/aroA | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate | Coding | 1290 |
| BBa_K2586019/gat | Glyphosate N-acetyl-transferase | Coding | 480 |
| BBa_K2586020/aroA* | Mutated aroA | Coding | 480 |