Difference between revisions of "Team:WPI Worcester/Results"

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<div>
 
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<h2> Antifreeze Proteins against Biofilm Formation </h2>
 
<h2> Antifreeze Proteins against Biofilm Formation </h2>
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<h3> HIStag Purification </h3>
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<h3> Protein Concentration Assay </h3>
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<h3> SDS Page Gel</h3>
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<h3> Biofilm Assay Result </h3>
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<h3> Project Achievements </h3>
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<h2> Project Achievements </h2>
  
 
<p> We achieved a lot over the course of our project, both positive and negative results. Below are our lists of successes and failures.</p>
 
<p> We achieved a lot over the course of our project, both positive and negative results. Below are our lists of successes and failures.</p>
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<h4> Successes of ICEberg </h4>
 
<h4> Successes of ICEberg </h4>
 
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<ul>
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<li></li>
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<li> Successful Purification of Antifreeze proteins in BL21 strains of E. coli </li>
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<li></li>
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<li> Reduction of Biofilm formation with any application of </li>
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<li> Transformation of EMG cells to express Antifreeze protein </li>
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<li> Production of a functional and inexpensive Gene Gun </li>
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<li> Transformed lettuce leaves to express GFP using the team Gene Gun </li>
 
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</ul>
  
 
<h4> Failures of ICEberg </h4>
 
<h4> Failures of ICEberg </h4>
 
<ul>
 
<ul>
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<li> Inability to make competent NCTC cells with multiple protocols </li>
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<li> IPTG inducible operon did not turn on </li>
 
</ul>
 
</ul>
  

Revision as of 17:25, 12 October 2018

Results

Biofilm Quantification

Antifreeze Proteins against Biofilm Formation

HIStag Purification

Protein Concentration Assay

SDS Page Gel

Biofilm Assay Result

Curcumin Assays

Lettuce Colony Counts

Gene Gun Transformation Results

Transformation with Antifreeze Proteins

Project Achievements

We achieved a lot over the course of our project, both positive and negative results. Below are our lists of successes and failures.

Successes of ICEberg

  • Successful Purification of Antifreeze proteins in BL21 strains of E. coli
  • Reduction of Biofilm formation with any application of
  • Transformation of EMG cells to express Antifreeze protein
  • Production of a functional and inexpensive Gene Gun
  • Transformed lettuce leaves to express GFP using the team Gene Gun

Failures of ICEberg

  • Inability to make competent NCTC cells with multiple protocols
  • IPTG inducible operon did not turn on