Difference between revisions of "Team:WPI Worcester/Results"

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<h2> Curcumin Assays </h2>
 
<h2> Curcumin Assays </h2>
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As can be seen, the EMG strain had the most pronounced biofilm development. While DH5α had an apparent drop in biofilm development, it like S. epidermidis and S. aureus showed an increase in biofilm growth. NCTC similarly shows an initial drop but an uptick in measured biofilm after 20 µM curcumin solution was applied. However, overlap between error bars in these four aforementioned strains among ratios means overall measurements may be suspect.
 
As can be seen, the EMG strain had the most pronounced biofilm development. While DH5α had an apparent drop in biofilm development, it like S. epidermidis and S. aureus showed an increase in biofilm growth. NCTC similarly shows an initial drop but an uptick in measured biofilm after 20 µM curcumin solution was applied. However, overlap between error bars in these four aforementioned strains among ratios means overall measurements may be suspect.

Revision as of 17:32, 12 October 2018

Results

Biofilm Quantification

Antifreeze Proteins against Biofilm Formation

HIStag Purification

Protein Concentration Assay

SDS Page Gel

Biofilm Assay Result

Curcumin Assays

As can be seen, the EMG strain had the most pronounced biofilm development. While DH5α had an apparent drop in biofilm development, it like S. epidermidis and S. aureus showed an increase in biofilm growth. NCTC similarly shows an initial drop but an uptick in measured biofilm after 20 µM curcumin solution was applied. However, overlap between error bars in these four aforementioned strains among ratios means overall measurements may be suspect.

Lettuce Colony Counts

Gene Gun Transformation Results

Transformation with Antifreeze Proteins

Project Achievements

We achieved a lot over the course of our project, both positive and negative results. Below are our lists of successes and failures.

Successes of ICEberg

  • Successful Purification of Antifreeze proteins in BL21 strains of E. coli
  • Reduction of Biofilm formation with any application of
  • Transformation of EMG cells to express Antifreeze protein
  • Production of a functional and inexpensive Gene Gun
  • Transformed lettuce leaves to express GFP using the team Gene Gun

Failures of ICEberg

  • Inability to make competent NCTC cells with multiple protocols
  • IPTG inducible operon did not turn on