Difference between revisions of "Team:WPI Worcester/InterLab"

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<h3>★  ALERT! </h3>
 
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<p>This page is used by the judges to evaluate your team for the <a href="https://2018.igem.org/Judging/Medals">medal criterion</a> or <a href="https://2018.igem.org/Judging/Awards"> award listed below</a>. </p>
 
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<p> Delete this box in order to be evaluated for this medal criterion and/or award. See more information at <a href="https://2018.igem.org/Judging/Pages_for_Awards"> Instructions for Pages for awards</a>.</p>
 
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<h1> InterLab Study </h1>
  
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<h3>Summary</h3>
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<p> As a team, we successfully completed the interlab study. We started with a standard of LUDOX absorption and used the data from the four replicates to construct standard curve for absorbance vs. particle number. We then used a serial dilution of fluorescein to construct a standard curve for fluorescence vs. fluorescein concentration.  A positive control, a negative control, and six different devices that contain GFP were tested experimentally and the values from the standard curves allowed us to find the fluorescence per particle. See the attached excel sheets for complete data tables and all of our raw data.</p>
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<a href= "https://static.igem.org/mediawiki/2018/4/42/T--WPI_Worcester--InterlabStudy.pdf" Excel Sheets /a>
  
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<h1>InterLab</h1>
 
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<h3>Bronze Medal Criterion #4</h3>
 
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<p><b>Standard Tracks:</b> Participate in the Interlab Measurement Study and/or obtain new, high quality experimental characterization data for an existing BioBrick Part or Device and enter this information on that part's Main Page in the Registry. The part that you are characterizing must NOT be from a 2018 part number range.
 
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For teams participating in the <a href="https://2018.igem.org/Measurement/InterLab">InterLab study</a>, all work must be shown on this page.
 
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Revision as of 20:16, 12 October 2018

InterLab Study

Summary

As a team, we successfully completed the interlab study. We started with a standard of LUDOX absorption and used the data from the four replicates to construct standard curve for absorbance vs. particle number. We then used a serial dilution of fluorescein to construct a standard curve for fluorescence vs. fluorescein concentration. A positive control, a negative control, and six different devices that contain GFP were tested experimentally and the values from the standard curves allowed us to find the fluorescence per particle. See the attached excel sheets for complete data tables and all of our raw data.