Difference between revisions of "Team:CCU Taiwan/Demonstrate"
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<div class="content"> | <div class="content"> | ||
<p class="first">Demonstration</p> | <p class="first">Demonstration</p> | ||
| − | <p class="description">We have not yet achieved the final goal we set at the beginning, but have achieved a high level of success. | + | <p class="description">  We have not yet achieved the final goal we set at the beginning, but have achieved a high level of success. |
</p> | </p> | ||
<img width="80%" style="background-color:white" src="https://static.igem.org/mediawiki/2018/3/3e/T--CCU_Taiwan--LIGGREEN.png"></img> | <img width="80%" style="background-color:white" src="https://static.igem.org/mediawiki/2018/3/3e/T--CCU_Taiwan--LIGGREEN.png"></img> | ||
| − | <p class="description">In terms of gene design, our goal is mainly the production of plant enzymes. Therefore, we demonstrated the growth curve of the yeast and confirmed the enzyme production. | + | <p class="description">  In terms of gene design, our goal is mainly the production of plant enzymes. Therefore, we demonstrated the growth curve of the yeast and confirmed the enzyme production. |
</p> | </p> | ||
<p class="second">Zeocin selection:</p> | <p class="second">Zeocin selection:</p> | ||
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</p> | </p> | ||
| − | <p class="description">In our modeling, we constructed a theoretical calculation for our material. Based on published literature, we used Spartan to determine the spontaneous basis for our bond reaction, and then Flory-Stockmayer theory to provide a theoretical estimate of the polymerization. Through the establishment of the model, we can get theoretical data on Liggreen. In patent applications and product improvements, we can improve on this estimate through theoretical calculations and material measurements. | + | <p class="description">  In our modeling, we constructed a theoretical calculation for our material. Based on published literature, we used Spartan to determine the spontaneous basis for our bond reaction, and then Flory-Stockmayer theory to provide a theoretical estimate of the polymerization. Through the establishment of the model, we can get theoretical data on Liggreen. In patent applications and product improvements, we can improve on this estimate through theoretical calculations and material measurements. |
</p> | </p> | ||
<img width="70%" src="https://static.igem.org/mediawiki/2018/c/cb/T--CCU_Taiwan--de3.png"></img> | <img width="70%" src="https://static.igem.org/mediawiki/2018/c/cb/T--CCU_Taiwan--de3.png"></img> | ||
| Line 158: | Line 158: | ||
</p> | </p> | ||
| − | <p class="description">We have designed a production line to produce Liggreen. Using existing fermentation tank systems and reaction tank assemblies on the market, we can construct our production line. | + | <p class="description">  We have designed a production line to produce Liggreen. Using existing fermentation tank systems and reaction tank assemblies on the market, we can construct our production line. |
</p> | </p> | ||
<img width="70%" src="https://static.igem.org/mediawiki/2018/0/02/T--CCU_Taiwan--de1.png"></img> | <img width="70%" src="https://static.igem.org/mediawiki/2018/0/02/T--CCU_Taiwan--de1.png"></img> | ||
| − | <p class="description">We have already done a market survey of Liggreen. In response to the needs of the commodity market, we have discussed several practical business models and various analyses. Furthermore, we have visited several companies that produce laminated paper cups, as well as several companies that are concerned about new environmentally friendly materials. After these visits, our project has been further improved and more in line with social needs. | + | <p class="description">  We have already done a market survey of Liggreen. In response to the needs of the commodity market, we have discussed several practical business models and various analyses. Furthermore, we have visited several companies that produce laminated paper cups, as well as several companies that are concerned about new environmentally friendly materials. After these visits, our project has been further improved and more in line with social needs. |
</p> | </p> | ||
<img width="70%" src="https://static.igem.org/mediawiki/2018/b/bd/T--CCU_Taiwan--de2.png"></img> | <img width="70%" src="https://static.igem.org/mediawiki/2018/b/bd/T--CCU_Taiwan--de2.png"></img> | ||
| − | <p class="description">In the future, we will make Liggreen not only in the laboratory but also meet the production standards of industry. We will promote the products to the public and fulfill the concept of environmental protection. | + | <p class="description">  In the future, we will make Liggreen not only in the laboratory but also meet the production standards of industry. We will promote the products to the public and fulfill the concept of environmental protection. |
</p><br> | </p><br> | ||
<br> | <br> | ||
Revision as of 05:40, 13 October 2018
Demonstration
We have not yet achieved the final goal we set at the beginning, but have achieved a high level of success.
In terms of gene design, our goal is mainly the production of plant enzymes. Therefore, we demonstrated the growth curve of the yeast and confirmed the enzyme production.
Zeocin selection:
In terms of Liggreen production, we also completed two important parts.
PART 1.
Spontaneous reaction of monolignols & calculation of Flory-Stockmayer Theory of polymerization
In our modeling, we constructed a theoretical calculation for our material. Based on published literature, we used Spartan to determine the spontaneous basis for our bond reaction, and then Flory-Stockmayer theory to provide a theoretical estimate of the polymerization. Through the establishment of the model, we can get theoretical data on Liggreen. In patent applications and product improvements, we can improve on this estimate through theoretical calculations and material measurements.
Three Bonds based on Spartan Analysis
PART 2.
Production line
We have designed a production line to produce Liggreen. Using existing fermentation tank systems and reaction tank assemblies on the market, we can construct our production line.
We have already done a market survey of Liggreen. In response to the needs of the commodity market, we have discussed several practical business models and various analyses. Furthermore, we have visited several companies that produce laminated paper cups, as well as several companies that are concerned about new environmentally friendly materials. After these visits, our project has been further improved and more in line with social needs.
In the future, we will make Liggreen not only in the laboratory but also meet the production standards of industry. We will promote the products to the public and fulfill the concept of environmental protection.