Difference between revisions of "Team:CCU Taiwan/Model"
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<li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a><hr> | <li class="title" style="cursor:pointer;" id="Home"><img class="img_title" src="https://static.igem.org/mediawiki/2018/2/24/T--CCU_Taiwan--aboutus.png"></img><a>About Us</a><hr> | ||
<ul class="sub" id="sub_home" style="cursor:default;"> | <ul class="sub" id="sub_home" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Team"><li class="list" id="home1">Team</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Attributions"><li class="list" id="home2">Attributions</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Medal"><li class="list" id="home2">Medals</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Judge"><li class="list" id="home3">For Judges</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
<li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a><hr> | <li class="title" style="cursor:pointer;" id="Project"><img class="img_title" src="https://static.igem.org/mediawiki/2018/6/6f/T--CCU_Taiwan--project.png"></img><a>Project</a><hr> | ||
<ul class="sub" id="sub_project" style="cursor:default;"> | <ul class="sub" id="sub_project" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Description"><li class="list" id="project1">Description</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Applied_Design"><li class="list" id="project2">Design</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Results"><li class="list" id="project3">Results</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Demonstrate"><li class="list" id="project6">Demonstration</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/InterLab "><li class="list" id="project5">InterLab</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
<li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a><hr> | <li class="title" style="cursor:pointer;" id="Parts"><img class="img_title" src="https://static.igem.org/mediawiki/2018/1/17/T--CCU_Taiwan--part.png"></img><a>Parts</a><hr> | ||
<ul class="sub" id="sub_parts" style="cursor:default;"> | <ul class="sub" id="sub_parts" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Parts"><li class="list" id="parts1">Overview</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Basic_Part"><li class="list" id="parts2">Basic Part</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Composite_Part"><li class="list" id="parts3">Composite Part</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Improve"><li class="list" id="parts4">Improved Part</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
<li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a><hr> | <li class="title" style="cursor:pointer;" id="Modeling"><img class="img_title" src="https://static.igem.org/mediawiki/2018/0/09/T--CCU_Taiwan--model.png"></img><a>Modeling</a><hr> | ||
<ul class="sub" id="sub_modeling" style="cursor:default;"> | <ul class="sub" id="sub_modeling" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Model"><li class="list" id="model1">Overview</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Binding"><li class="list" id="model2">Binding</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Polymer"><li class="list" id="model4">Polymer</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Degradation"><li class="list" id="model3">Degradation</li></a> | |
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</ul> | </ul> | ||
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| − | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a> | + | <li class="title" style="cursor:pointer;" id="Drylab"><img class="img_title" src="https://static.igem.org/mediawiki/2018/f/fc/T--CCU_Taiwan--Dry_lab.png"></img><a>Product</a><hr> |
<ul class="sub" id="sub_drylab" style="cursor:default;"> | <ul class="sub" id="sub_drylab" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Our_Plan"><li class="list" id="drylab1">Analysis</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Engineering"><li class="list" id="drylab2">Production Line</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Safety"><li class="list" id="drylab3">Safety</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
<li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a><hr> | <li class="title" style="cursor:pointer;" id="Human_Practice"><img class="img_title" src="https://static.igem.org/mediawiki/2018/9/96/T--CCU_Taiwan--humanpractice.png"></img><a>HP</a><hr> | ||
<ul class="sub" id="sub_human_practice" style="cursor:default;"> | <ul class="sub" id="sub_human_practice" style="cursor:default;"> | ||
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Human_Practices"><li class="list" id="human_practice1">Overview</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Public_Engagement"><li class="list" id="human_practice2">Public Engagement</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Entrepreneurship"><li class="list" id="human_practice3">Entrepreneurship</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Intergrate"><li class="list" id="human_practice4">Intergrated HP</li></a> | |
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</ul> | </ul> | ||
</li> | </li> | ||
<li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a> | <li class="title" style="cursor:pointer;" id="Notebook"><img class="img_title" src="https://static.igem.org/mediawiki/2018/c/c9/T--CCU_Taiwan--notebook.png"></img><a>Notebook</a> | ||
<ul class="sub" id="sub_notebook" style="cursor:default;"> | <ul class="sub" id="sub_notebook" style="cursor:default;"> | ||
| − | <li class="list" id="notebook1"><a href="https://2018.igem.org/Team:CCU_Taiwan/ | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Notebook"><li class="list" id="notebook1">Overview</li></a> |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Collaborations"><li class="list" id="notebook2">Collaborations</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Protocols"><li class="list" id="notebook2">Protocols</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Experiments"><li class="list" id="notebook3">Experiments</li></a> | |
| − | + | <a href="https://2018.igem.org/Team:CCU_Taiwan/Materials"><li class="list" id="notebook4">Materials</li></a> | |
</ul> | </ul> | ||
</li> | </li> | ||
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</header> | </header> | ||
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<div class="backgroundModeling"> | <div class="backgroundModeling"> | ||
<div class="photoModeling"></div> | <div class="photoModeling"></div> | ||
Revision as of 23:55, 13 October 2018
Modeling Overview
In the future, there will be fewer and fewer petrochemical resources on the planet, and plastic waste will accumulate. When things change, what should we do? To solve the problem, we use Liggreen. We hope to establish a new production line of laminating coating through biological methods. First of all, we use natural enzyme to synthesize the material. Through the synthetic reaction of enzyme and monolignols, we make our Liggreen structure be close to natural vegetation. Then, we could create a biological coating which will not require petrochemicals and high energy consumption. Our modeling is mainly divided into three parts to prove our project – Enzyme production, Monomer polymerization, Molecular prediction.
Enzyme production:
Transforming p.pastoris by synthetic biology. We want to make yeast produce the required enzymes – Lac1, Px16, Px18
Modeling: Proving the experiment by measuring the growth curve of p. pastoris and auto selection. Then using the different tag genes to design for western blot, proving the success of our overall enzyme production system.
Monomer polymerization:
The three enzymes are added into the monolignols. And the monolignols will generate free radicals and bond to each other.
Modeling: We use Spartan to simulate the monolignol and the primary product, and then the free energy is obtained. The experiment was used to verify the spontaneity of the reaction.
Molecular prediction:
When the polymerization reaction is carried out to a certain extent, we would measure the physicochemical properties and carry out the modification. So that lignin can be better used in the coating industry.
Modeling: In this part, because we can't complete the all of polymerization reaction, we refer to paper for the estimation of molecular polymerization products. The estimation is mainly based on three parts – monomer, bond and product molecular weight. Since we mainly use the monolignol G for the reaction first, the structural estimation is mainly based on the Epoxy Resin.