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| | <div class="photoDemonstration"></div> | | <div class="photoDemonstration"></div> |
| | <div class="content"> | | <div class="content"> |
| − | <p class="description">  We have not yet achieved the final goal we set at the beginning, but have achieved a high level of success. | + | <p class="description">  We aim to create a new material for laminating paper cups. This new film, which we call “Liggreen”, is similar to lignin and eco-friendly. We hope that cups laminated with Liggreen will be not only waterproof and heat-resistant but also biodegradable. It is a breakthrough attempt to replace current paper cup lamination based on petrochemical materials such as Polypropylene and Polyethylene. </p> |
| − | </p>
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| − | <img width="80%" style="background-color:white" src="https://static.igem.org/mediawiki/2018/3/3e/T--CCU_Taiwan--LIGGREEN.png"></img>
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| − | <p class="description">  In terms of gene design, our goal is mainly the production of plant enzymes. Therefore, we demonstrated the growth curve of the yeast and confirmed the enzyme production.
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| − | </p>
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| − | <p class="second">Zeocin selection:</p>
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| − | <img width="50%" src="https://static.igem.org/mediawiki/2018/2/21/T--CCU_Taiwan--de2_1.png"></img>
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| − | <img width="50%" src="https://static.igem.org/mediawiki/2018/f/fa/T--CCU_Taiwan--de2_2.png"></img>
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| − | <img width="50%" src="https://static.igem.org/mediawiki/2018/0/0b/T--CCU_Taiwan--de2_3.png"></img>
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| − | <p class="description">In terms of Liggreen production, we also completed two important parts.
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| − | </p>
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| − | <p class="second">PART 1.<br>
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| − | Spontaneous reaction of monolignols & calculation of Flory-Stockmayer Theory of polymerization
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| − | | + | |
| − | </p>
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| − | <p class="description">  In our modeling, we constructed a theoretical calculation for our material. Based on published literature, we used Spartan to determine the spontaneous basis for our bond reaction, and then Flory-Stockmayer theory to provide a theoretical estimate of the polymerization. Through the establishment of the model, we can get theoretical data on Liggreen. In patent applications and product improvements, we can improve on this estimate through theoretical calculations and material measurements.
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| − | </p>
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| − | <img width="70%" src="https://static.igem.org/mediawiki/2018/c/cb/T--CCU_Taiwan--de3.png"></img>
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| − | <p class="description" style="color:red">Three Bonds based on Spartan Analysis</p>
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| − | <p class="second">PART 2.<br>
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| − | Production line
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| − | | + | |
| − | </p>
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| − | <p class="description">  We have designed a production line to produce Liggreen. Using existing fermentation tank systems and reaction tank assemblies on the market, we can construct our production line.
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| − | </p>
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| − | <img width="70%" src="https://static.igem.org/mediawiki/2018/0/02/T--CCU_Taiwan--de1.png"></img>
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| − | <p class="description">  We have already done a market survey of Liggreen. In response to the needs of the commodity market, we have discussed several practical business models and various analyses. Furthermore, we have visited several companies that produce laminated paper cups, as well as several companies that are concerned about new environmentally friendly materials. After these visits, our project has been further improved and more in line with social needs.
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| − | </p>
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| − | <img width="70%" src="https://static.igem.org/mediawiki/2018/b/bd/T--CCU_Taiwan--de2.png"></img>
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| − | <p class="description">  In the future, we will make Liggreen not only in the laboratory but also meet the production standards of industry. We will promote the products to the public and fulfill the concept of environmental protection.
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| − | </p><br> | + | |
| − | <br>
| + | |
| − | <br>
| + | |
| | </div> | | </div> |
| | </div> | | </div> |
