Difference between revisions of "Team:Evry Paris-Saclay/Parts"
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<h2 class="anchor" style="font-weight:800; text-align:center;" id='basic_parts'>BASIC PARTS</h2> | <h2 class="anchor" style="font-weight:800; text-align:center;" id='basic_parts'>BASIC PARTS</h2> | ||
| − | <p>During this project, our team designed the following basic | + | <p style="text-align:center">During this project, our team designed the following basic parts:</p> |
| + | <h3 style="font-weight:800; text-align:center;">Coding sequences</h3> | ||
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| + | <h3 style="font-weight:800; text-align:center;">Ribosomal binding sites (RBS)</h3> | ||
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| + | <h3 style="font-weight:800; text-align:center;">Promoters</h3> | ||
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| + | <h3 style="font-weight:800; text-align:center;">Terminators</h3> | ||
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| + | <h3 style="font-weight:800; text-align:center;">and a Golden Gate adapter</h3> | ||
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<h2 class="anchor" style="font-weight:800; text-align:center;" id='composite_parts'>COMPOSITE PARTS</h2> | <h2 class="anchor" style="font-weight:800; text-align:center;" id='composite_parts'>COMPOSITE PARTS</h2> | ||
| + | <p style="text-align:center">During this project, our team designed the following composite parts:</p> | ||
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<h2 class="anchor" style="font-weight:800; text-align:center;" id='part_collection'>PART COLLECTION</h2> | <h2 class="anchor" style="font-weight:800; text-align:center;" id='part_collection'>PART COLLECTION</h2> | ||
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| + | <p style="font-size:15px;">In our project we tried to repurpose a peptide communication machinery from a phage of the Gram-positive Bacillus subtilis to the cells of Gram-negative Escherichia coli, and quickly discovered that crossing the inter-species barrier in bioengineering is a challenging task. This Part Collection consists of a series of promoter variants designed to debug the mechanism of transcriptional regulation in the aimX promoter that is key to the signalling system. The Collection helped us successfully identify an internal terminator, investigate a potential anti-termination mechanism, and build a hybrid promoter that converts the native transcriptional activator (AimR) into a transcriptional repressor. When placed upstream of a reporter gene, promoters from this Part Collection showed variable transcription strengths and degrees of regulation that provided valuable insights for our project and facilitated the porting of the peptide communication machinery from B. subtilis to E. coli (BBa_K2675050 to K2675065 & BBa_K2675190 to BBa_K2675197).</p> | ||
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<div class="column full_size"> | <div class="column full_size"> | ||
Revision as of 09:44, 15 October 2018
PARTS
BASIC PARTS
During this project, our team designed the following basic parts:
Coding sequences
Ribosomal binding sites (RBS)
Promoters
Terminators
and a Golden Gate adapter
COMPOSITE PARTS
During this project, our team designed the following composite parts:
PART COLLECTION
In our project we tried to repurpose a peptide communication machinery from a phage of the Gram-positive Bacillus subtilis to the cells of Gram-negative Escherichia coli, and quickly discovered that crossing the inter-species barrier in bioengineering is a challenging task. This Part Collection consists of a series of promoter variants designed to debug the mechanism of transcriptional regulation in the aimX promoter that is key to the signalling system. The Collection helped us successfully identify an internal terminator, investigate a potential anti-termination mechanism, and build a hybrid promoter that converts the native transcriptional activator (AimR) into a transcriptional repressor. When placed upstream of a reporter gene, promoters from this Part Collection showed variable transcription strengths and degrees of regulation that provided valuable insights for our project and facilitated the porting of the peptide communication machinery from B. subtilis to E. coli (BBa_K2675050 to K2675065 & BBa_K2675190 to BBa_K2675197).
Part Table
Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.
<groupparts>iGEM18 Evry_Paris-Saclay</groupparts>