Difference between revisions of "Team:Evry Paris-Saclay/Parts"

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<p style="text-align:center">During this project, our team designed the following basic parts:</p>
 
<p style="text-align:center">During this project, our team designed the following basic parts:</p>
  
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   <tr>
 
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       <th>Part Number</th>
 
       <th>Part Number</th>
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       <td><a href=" http://parts.igem.org/Part:BBa_K2675000 target=__blank">BBa_K2675000 </a></td>
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       <td><a href=" http://parts.igem.org/Part:BBa_K2675000  target=__blank">BBa_K2675000 </a></td>
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       <td> AimR-Phi3T </td>   </tr>
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       <td> AimR-Phi3T </td>
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  </tr>
 
   <tr>
 
   <tr>
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      <td><a href=" http://parts.igem.org/Part:BBa_K2675001  target=__blank">BBa_K2675001 </a></td>
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      <td> AimP-Phi3T </td>
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  </tr>
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  <tr>
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      <td><a href=" http://parts.igem.org/Part:BBa_K2675002  target=__blank">BBa_K2675002 </a></td>
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      <td> OmpA-SAIRGA </td>
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  </tr>
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  <tr>
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      <td><a href=" http://parts.igem.org/Part:BBa_K2675003  target=__blank">BBa_K2675003 </a></td>
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      <td> PelB-SAIRGA </td>
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  </tr>
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  <tr>
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      <td><a href=" http://parts.igem.org/Part:BBa_K2675004  target=__blank">BBa_K2675004 </a></td>
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      <td> TAT-SAIRGA </td>
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  </tr>
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  <tr>
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      <td><a href=" http://parts.igem.org/Part:BBa_K2675005  target=__blank">BBa_K2675005 </a></td>
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      <td> sfGFP </td>
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  </tr>
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  <tr>
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      <td><a href=" http://parts.igem.org/Part:BBa_K2675006  target=__blank">BBa_K2675006 </a></td>
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      <td> sfGFP-LVAtag </td>
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  </tr>
 
</table>
 
</table>
  

Revision as of 10:00, 15 October 2018


TO CONTACT US
Genopole Campus 1, Batiment 6, 91030 Evry Cedex, France
+33 7 69 96 68 31
igemevry@gmail.com

© Copyright 2018
Design & Developpement by
IGEM EVRY GENOPOLE

► Overview ► Basic Parts ► Composite Parts ► Part Collection

PARTS

BASIC PARTS

During this project, our team designed the following basic parts:

Part Number Part Name
BBa_K2675000 AimR-Phi3T
BBa_K2675001 AimP-Phi3T
BBa_K2675002 OmpA-SAIRGA
BBa_K2675003 PelB-SAIRGA
BBa_K2675004 TAT-SAIRGA
BBa_K2675005 sfGFP
BBa_K2675006 sfGFP-LVAtag

Coding sequences

Ribosomal binding sites (RBS)

Promoters

Terminators

and a Golden Gate adapter

COMPOSITE PARTS

During this project, our team designed the following composite parts:

PART COLLECTION

In our project we tried to repurpose a peptide communication machinery from a phage of the Gram-positive Bacillus subtilis to the cells of Gram-negative Escherichia coli, and quickly discovered that crossing the inter-species barrier in bioengineering is a challenging task. This Part Collection consists of a series of promoter variants designed to debug the mechanism of transcriptional regulation in the aimX promoter that is key to the signalling system. The Collection helped us successfully identify an internal terminator, investigate a potential anti-termination mechanism, and build a hybrid promoter that converts the native transcriptional activator (AimR) into a transcriptional repressor. When placed upstream of a reporter gene, promoters from this Part Collection showed variable transcription strengths and degrees of regulation that provided valuable insights for our project and facilitated the porting of the peptide communication machinery from B. subtilis to E. coli (BBa_K2675050 to K2675065 & BBa_K2675190 to BBa_K2675197).

Part Table

Please include a table of all the parts your team has made during your project on this page. Remember part characterization and measurement data must go on your team part pages on the Registry.

<groupparts>iGEM18 Evry_Paris-Saclay</groupparts>