Difference between revisions of "Team:SKLMT-China/Notebook Overview"
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<section class="article-banner" style="background-image: url(https://static.igem.org/mediawiki/2018/8/8f/T--SKLMT-China--arttest-banner-1.jpg)"> | <section class="article-banner" style="background-image: url(https://static.igem.org/mediawiki/2018/8/8f/T--SKLMT-China--arttest-banner-1.jpg)"> | ||
<div class="banner-content"> | <div class="banner-content"> | ||
| − | <h2 class="title"> | + | <h2 class="title">NOTEBOOK</h2> |
| − | <p class="content"> | + | <p class="content">overview</p> |
</div> | </div> | ||
</section> | </section> | ||
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<h4 class="panel-title"> | <h4 class="panel-title"> | ||
<a data-toggle="collapse" data-parent="#accordion" href="#collapseOne"> | <a data-toggle="collapse" data-parent="#accordion" href="#collapseOne"> | ||
| − | + | week1 | |
</a> | </a> | ||
</h4> | </h4> | ||
Revision as of 10:21, 15 October 2018
| MasterMix | ||
|---|---|---|
| Cut Smart buffer | (n + 1) x | 0.2 µl |
| PvuII-HF | (n + 1) x | 0.2 µl |
| dH2O | (n + 1) x | 4.8 |
| Total volume | (n + 1) x | 6.0 µl |
• Add 6.0 µl of the Mastermix to 4.0 µl of the dissolved plasmid DNA
• Incubate for at least 1.5 h at 37°C.
• Load the whole mixture with loading buffer on a 0.7% agarose gel (containing EtBr) and perform electrophoresis. Take a picture.