Difference between revisions of "Team:TecMonterrey GDL/Improve"

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<h1>Improve</h1>
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<p>For teams seeking to improve upon a previous part or project, you should document all of your work on this page. Please remember to include all part measurement and characterization data on the part page on the Registry. Please include a link to your improved part on this page.</p>
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<h3>Gold Medal Criterion #2</h3>
 
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<p><b>Standard Tracks:</b> Create a new part that has a functional improvement upon an existing BioBrick part. The sequences of the new and existing parts must be different. You must perform experiments with both parts to demonstrate this improvement.  Document the experimental characterization on the Part's Main Page on the Registry for both the existing and new parts. Both the new and existing Main Page of each Part’s Registry entry must reference each other. Submit a sample of the new part to the Registry.
 
  
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The existing part must NOT be from your 2018 part number range and must be different from the part documented in bronze #4.
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<b>Special Tracks:</b> Improve the function of an existing iGEM project (that your current team did not originally create) and display your achievement on your wiki.</p>
 
  
  
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            <h1>Improvement of parts</h1>
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                    <p>The team managed to gather sequences in the registry and modified them in order to improve a) purification and b) could be used in Lactobacillus rhamnosus species; since the registry contains mainly
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                      E. coli parts, it might be useful as well, to design and obtain sequences that can be expressed on one or the other but both.
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                      The following sequences were codon optimized for use in the previous microorganisms mentioned.</p>
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                  <p>We managed to test some of these pieces when characterizing our signal peptide for secretion of
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                    recombinant proteins collection, (visit parts) , and we obtained the following results: </p>
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Revision as of 09:13, 17 October 2018

TecMonterrey_GDL

Improvement of parts

The team managed to gather sequences in the registry and modified them in order to improve a) purification and b) could be used in Lactobacillus rhamnosus species; since the registry contains mainly E. coli parts, it might be useful as well, to design and obtain sequences that can be expressed on one or the other but both. The following sequences were codon optimized for use in the previous microorganisms mentioned.

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We managed to test some of these pieces when characterizing our signal peptide for secretion of recombinant proteins collection, (visit parts) , and we obtained the following results:



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TecMonterrey_GDL