Difference between revisions of "Team:SKLMT-China/Basic Part"

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        <h2 class="title">Overview </h2>
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<p>Our submitted parts include a promoter library of inner promoters with various strength built in latin<>Pseudomonas fluorescence</latin>-pf5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in  enhanced nicotine degradation compared to original nicA2 gene. </p>
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            <h2 class="title">Integrated & Gold</h2>
          <h2 class="title"> Promoter Library</2>
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             <p class="content">What is four revolution?</p>
             <p>We are proud to submit a promoter library of inner promoters with various strength built in <i>Pseudomonas fluorescence</i>-pf5. And these promoters belong to our basic parts. They are so lovely and good boys, which enable future iGEM teams to easily and predictably get protein production in <i>Pseudomonas fluorescence</i>-pf5. Additionally, this promoter library will support future iGEM teams some help in exploration on <i>Pseudomonas fluorescence</i>-pf5 cells with high capacity in degrading. </p>
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<p>We explored these promoters by measuring strength of firefly fluorescein, marked as “y” data.</p>
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<img src="https://static.igem.org/mediawiki/2018/9/9b/T--SKLMT-China--promoter_y.png" alt="Measurement of promoter strength via measuring firefly fluorescein" />
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<p>According to friend teams’ collaboration datas and our analysing, we built a model to regularize and predict the strength of library members. You can learn about these members intuitively. </p>
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<img src="https://static.igem.org/mediawiki/2018/e/e3/T--SKLMT-China--promoter_outputy.png
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  <h2 class="title"> All Basic Parts</2>  
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<table><thead><tr><td>Biobrick</td><td>Type</td><td>Description</td><td>length</td></tr><tr><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td><td>&nbsp;</td></tr></thead><tbody><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569001">BBa_K2569001</a></td><td>Regulatory</td><td>Pdiv, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569002">BBa_K2569002</a></td><td>Regulatory</td><td>ParaA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569003">BBa_K2569003</a></td><td>Regulatory</td><td>PfabD, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569005">BBa_K2569005</a></td><td>Regulatory</td><td>PccmD, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569006">BBa_K2569006</a></td><td>Regulatory</td><td>PfliA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569011">BBa_K2569011</a></td><td>Regulatory</td><td>PpltR, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569012">BBa_K2569012</a></td><td>Regulatory</td><td>PproC, constitutive  promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569013">BBa_K2569013</a></td><td>Regulatory</td><td>PrecA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569014">BBa_K2569014</a></td><td>Regulatory</td><td>PrpoD, a constitutive promoter in pseudomonas fluorescence</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569015">BBa_K2569015</a></td><td>Regulatory</td><td>PrpoS, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569016">BBa_K2569016</a></td><td>Regulatory</td><td>PdnaA, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569017">BBa_K2569017</a></td><td>Regulatory</td><td>Ppol, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569018">BBa_K2569018</a></td><td>Regulatory</td><td>P16s, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569019">BBa_K2569019</a></td><td>Regulatory</td><td>Psig, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569020">BBa_K2569020</a></td><td>Regulatory</td><td>Patf, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569021">BBa_K2569021</a></td><td>Regulatory</td><td>Pfme, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569022">BBa_K2569022</a></td><td>Regulatory</td><td>Pmem, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569023">BBa_K2569023</a></td><td>Regulatory</td><td>Pfat, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569024">BBa_K2569024</a></td><td>Regulatory</td><td>Pami , constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569025">BBa_K2569025</a></td><td>Regulatory</td><td>Prib, constitutive promoter in pseudomonas fluorescence pf-5</td><td>70</td></tr><tr><td><a
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             <h2 class="title">Overview </h2>
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            <p>Our submitted parts include a promoter library of inner promoters with various strength built in <latin>Pseudomonas fluorescence</latin>-pf5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in  enhanced nicotine degradation compared to original nicA2 gene. </p>
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            <h2 class="title">Composite Parts</h2>
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            <table><thead><tr><td>Biobrick</td><td>Type</td><td>Description</td><td>length</td></tr></thead><tbody><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569031">BBa_K2569031</a></td><td>Composite</td><td>PdnaA -NicA2</td><td>1525</td></tr><tr><td><a href="http://parts.igem.org/wiki/index.php?title=Part:BBa_K2569032">BBa_K2569032</a></td><td>Composite</td><td>PdnaA-mRFP</td><td>782</td></tr></tbody></table><br/></p>
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Revision as of 13:42, 17 October 2018

Overview

Our submitted parts include a promoter library of inner promoters with various strength built in Pseudomonas fluorescence-pf5. We also include well-characterized composite part. One includes our strongest promoter and nicotine oxidase NicA2, working well in enhanced nicotine degradation compared to original nicA2 gene.

Composite Parts

BiobrickTypeDescriptionlength
BBa_K2569031CompositePdnaA -NicA21525
BBa_K2569032CompositePdnaA-mRFP782