Difference between revisions of "Team:SKLMT-China/Principle"

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             <p>This year, the team SKLMT-China established a useful software tool based on our own wet lab results to help people easily search and predict a proper promoter for fine-tuning gene expression in the synthetic study. To make the <latin>Pseudomonas fluorescence </latin>a well-exploited chassis bacteria in synthetic biology, we develop a software <b>DePro </b>(promoter searching and strength prediction website based on deep learning and python), which enables our research results to interact well with other teams. With the expansion of the promoter data, it can quickly calculate the strength level of the new promoter with the help of our model. After entering the core sequence, our python program will calculate the strength level of the promoter for you. In some way, the software<b>DePro </b>is a collection of our wet lab results and achievements.</p>
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             <p>We built our research into websites to address the need for interaction. </p>
<p>Our Depro system is a platform for promoter researchers to exchange data and share results. Everyone can benefit from the test results of previous researchers or enrich our software with new test data to improve the accuracy of the fit. Through our software, you can:</p>
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  <p>First, we processed the update module of the data. We use python language to process the input of DNA sequences, and use the coding format of the triple base to encode the core sequence. Subsequently, 65 data of the occurrence frequency and promoter strength of the AAA~GGG sequence are input into the database through library “pymysql”.</p>
<p>(1) Predict a new promoter’s strength. Our program is based on all known data for the deep learning of supervised training. you are merely asked to input the core sequence of the promoter, we will immediately calculate the strength level of the promoter</p>
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  <p>Next, we dealt with the part of deep learning. After users set learning generation on the Internet, our program will automatically retrieve all the data stored in the database, calculate all parameters according to the model of supervised training, and finally export the calculation results to the database for future use.</p>
<p>(2) Use a new set of data to enrich the program's deep learning process. Your data will be imported into our database and will accompany our program to complete every deep learning in the future</p>
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  <p>Finally, we provide an interface for predicting promoter strength. The user only needs to input the core sequence, and our program will read all the parameters obtained after deep learning from the database and calculate the strength of the promoter through the neural network structure we present. If the user has added new data before, re-complete the deep learning before the prediction session to feed the added data back to the results.</p>
<p>(3) Freely set the number of times to learn. You are free to decide how quickly and precisely your program will run to meet your needs</p>
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Revision as of 15:27, 17 October 2018

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introduction

We built our research into websites to address the need for interaction.

First, we processed the update module of the data. We use python language to process the input of DNA sequences, and use the coding format of the triple base to encode the core sequence. Subsequently, 65 data of the occurrence frequency and promoter strength of the AAA~GGG sequence are input into the database through library “pymysql”.

Next, we dealt with the part of deep learning. After users set learning generation on the Internet, our program will automatically retrieve all the data stored in the database, calculate all parameters according to the model of supervised training, and finally export the calculation results to the database for future use.

Finally, we provide an interface for predicting promoter strength. The user only needs to input the core sequence, and our program will read all the parameters obtained after deep learning from the database and calculate the strength of the promoter through the neural network structure we present. If the user has added new data before, re-complete the deep learning before the prediction session to feed the added data back to the results.



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