Difference between revisions of "Team:UCSC/Safety"

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<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
<p>Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?</p>
 
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</div>
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<br><br><br><br><br>
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Introduction to Importance of Safety
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  use iGEM stuff
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In Lab Safety
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  EH&S Standards
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    use 2015 desc
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  UCSC iGEM Standards
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    Lab Safety Course
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    buddy system
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    personal protection equipment inside lab
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    uphold EH&S standards
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Project Safety
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  Organisms (Biosafety All level 1 or less)
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    Yarrowia lipolytica (Candida lipolytica) - strain W29 (Level 1) (exists naturally in the mouth and gut, can lead to infections in immunocompromised people (rare).  GRAS organism)
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    7-dehydrocholesterol reductase - (reduces Ergosterol to Ergosta 5-eneol and/or Ergosta 5,22-dieneol)
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    adrenodoxin-NADP+ reductase (ADR) -one of the electron carriers needed to allow P450scc to function
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    ferredoxin-1 (FDX1) - the second electron carrier needed to allow P450scc to function
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    side-chain cleavage cytochrome (P450scc) - converts Ergostra 5-eneol and Ergostra 5,22-dieneol to pregnenolone
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    3beta-hydroxy-Delta5-steroid dehydrogenase/steroid - catalyzes the conversion of pregnenolone to progesterone
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Additional Safety Resources
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  CDC
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  NIH
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  ABSA
  
  

Revision as of 19:31, 2 July 2018

UCSC iGEM: Page In Progress

Hello! Thank you for visiting our website. This page is currently being developed, but check back for updates!

Safety

Please visit the Safety Hub to find this year's safety requirements & deadlines, and to learn about safe & responsible research in iGEM.

On this page of your wiki, you should write about how you are addressing any safety issues in your project. The wiki is a place where you can go beyond the questions on the safety forms, and write about whatever safety topics are most interesting in your project. (You do not need to copy your safety forms onto this wiki page.)

Safe Project Design

Does your project include any safety features? Have you made certain decisions about the design to reduce risks? Write about them here! For example:

  • Choosing a non-pathogenic chassis
  • Choosing parts that will not harm humans / animals / plants
  • Substituting safer materials for dangerous materials in a proof-of-concept experiment
  • Including an "induced lethality" or "kill-switch" device

Safe Lab Work

What safety procedures do you use every day in the lab? Did you perform any unusual experiments, or face any unusual safety issues? Write about them here!

Safe Shipment

Did you face any safety problems in sending your DNA parts to the Registry? How did you solve those problems?






Introduction to Importance of Safety use iGEM stuff In Lab Safety EH&S Standards use 2015 desc UCSC iGEM Standards Lab Safety Course buddy system personal protection equipment inside lab uphold EH&S standards Project Safety Organisms (Biosafety All level 1 or less) Yarrowia lipolytica (Candida lipolytica) - strain W29 (Level 1) (exists naturally in the mouth and gut, can lead to infections in immunocompromised people (rare). GRAS organism) 7-dehydrocholesterol reductase - (reduces Ergosterol to Ergosta 5-eneol and/or Ergosta 5,22-dieneol) adrenodoxin-NADP+ reductase (ADR) -one of the electron carriers needed to allow P450scc to function ferredoxin-1 (FDX1) - the second electron carrier needed to allow P450scc to function side-chain cleavage cytochrome (P450scc) - converts Ergostra 5-eneol and Ergostra 5,22-dieneol to pregnenolone 3beta-hydroxy-Delta5-steroid dehydrogenase/steroid - catalyzes the conversion of pregnenolone to progesterone Additional Safety Resources CDC NIH ABSA