Difference between revisions of "Team:TecMonterrey GDL/Protocols"
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<ul class="protocol-list"> | <ul class="protocol-list"> | ||
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<a href="https://static.igem.org/mediawiki/2018/2/26/T--TecMonterrey_GDL--Protocols--1._LB_medium_preparation.pdf">View Preparation of LB Medium</a> | <a href="https://static.igem.org/mediawiki/2018/2/26/T--TecMonterrey_GDL--Protocols--1._LB_medium_preparation.pdf">View Preparation of LB Medium</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Protocols--3._Transformation_by_electroporation.pdf">View Transformation by electroporation</a> | <a href="https://static.igem.org/mediawiki/2018/0/09/T--TecMonterrey_GDL--Protocols--3._Transformation_by_electroporation.pdf">View Transformation by electroporation</a> | ||
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</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2018/5/53/T--TecMonterrey_GDL--Protocols--1._3.1_Competent_cells_BL21.pdf">View Calcio competent cells</a> | <a href="https://static.igem.org/mediawiki/2018/5/53/T--TecMonterrey_GDL--Protocols--1._3.1_Competent_cells_BL21.pdf">View Calcio competent cells</a> | ||
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</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2018/a/a3/T--TecMonterrey_GDL--Protocols--3.3_Ligation_by_iGEM.pdf">View Ligation and digestion of G-Blocks</a> | <a href="https://static.igem.org/mediawiki/2018/a/a3/T--TecMonterrey_GDL--Protocols--3.3_Ligation_by_iGEM.pdf">View Ligation and digestion of G-Blocks</a> | ||
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</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2018/1/13/T--TecMonterrey_GDL--Protocols--3.4_Transformation_heat_shock_protocol.pdf">View Transformation Heat Shock</a> | <a href="https://static.igem.org/mediawiki/2018/1/13/T--TecMonterrey_GDL--Protocols--3.4_Transformation_heat_shock_protocol.pdf">View Transformation Heat Shock</a> | ||
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<ul class="protocol-list"> | <ul class="protocol-list"> | ||
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<a href="https://static.igem.org/mediawiki/2018/0/05/T--TecMonterrey_GDL--Protocols--1.1_LB_medium_and_LB_agar-plates_protocol.pdf">View LB medium and LB agar-plates Protocol</a> | <a href="https://static.igem.org/mediawiki/2018/0/05/T--TecMonterrey_GDL--Protocols--1.1_LB_medium_and_LB_agar-plates_protocol.pdf">View LB medium and LB agar-plates Protocol</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/c/c4/T--TecMonterrey_GDL--Protocols--4._Miniprep_kit.pdf">View MINIPREP KIT</a> | <a href="https://static.igem.org/mediawiki/2018/c/c4/T--TecMonterrey_GDL--Protocols--4._Miniprep_kit.pdf">View MINIPREP KIT</a> | ||
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</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2018/9/99/T--TecMonterrey_GDL--Protocols--5._PCR.pdf">View PCR</a> | <a href="https://static.igem.org/mediawiki/2018/9/99/T--TecMonterrey_GDL--Protocols--5._PCR.pdf">View PCR</a> | ||
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</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2018/0/0b/T--TecMonterrey_GDL--Protocols--5._Horizontal_electroforesis_protocol.pdf">View Agarose Gel Electrophoresis</a> | <a href="https://static.igem.org/mediawiki/2018/0/0b/T--TecMonterrey_GDL--Protocols--5._Horizontal_electroforesis_protocol.pdf">View Agarose Gel Electrophoresis</a> | ||
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</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2018/2/2a/T--TecMonterrey_GDL--Protocols--5._Optimizing_restriction_EcoR_I_y_Spe_I.pdf">View Optimizing Restriction Endonuclease Reactions</a> | <a href="https://static.igem.org/mediawiki/2018/2/2a/T--TecMonterrey_GDL--Protocols--5._Optimizing_restriction_EcoR_I_y_Spe_I.pdf">View Optimizing Restriction Endonuclease Reactions</a> | ||
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</ul> | </ul> | ||
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<ul class="protocol-list"> | <ul class="protocol-list"> | ||
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<a href="https://static.igem.org/mediawiki/2018/c/c0/T--TecMonterrey_GDL--Protocols--2._Electro_competent_cells_protocol.pdf">View Competent cells</a> | <a href="https://static.igem.org/mediawiki/2018/c/c0/T--TecMonterrey_GDL--Protocols--2._Electro_competent_cells_protocol.pdf">View Competent cells</a> | ||
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</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2018/4/4d/T--TecMonterrey_GDL--Protocols--6._Lysis_and_protein_purification.pdf">View Protein extraction and purification based on Thermo Fisher's Protocol</a> | <a href="https://static.igem.org/mediawiki/2018/4/4d/T--TecMonterrey_GDL--Protocols--6._Lysis_and_protein_purification.pdf">View Protein extraction and purification based on Thermo Fisher's Protocol</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/6/68/T--TecMonterrey_GDL--Protocols--7._SDS-PAGE_%2B_coomassie_staining_protocols.pdf">View SDS-PAGE</a> | <a href="https://static.igem.org/mediawiki/2018/6/68/T--TecMonterrey_GDL--Protocols--7._SDS-PAGE_%2B_coomassie_staining_protocols.pdf">View SDS-PAGE</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/0/0e/T--TecMonterrey_GDL--Protocols--8._Western_blot.pdf">View Western Blot based on Invitrogen's protocol</a> | <a href="https://static.igem.org/mediawiki/2018/0/0e/T--TecMonterrey_GDL--Protocols--8._Western_blot.pdf">View Western Blot based on Invitrogen's protocol</a> | ||
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</ul> | </ul> | ||
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<ul class="protocol-list"> | <ul class="protocol-list"> | ||
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<a href="https://static.igem.org/mediawiki/2018/5/53/T--TecMonterrey_GDL--Protocols--1._Electro_competent_cells_protocol.pdf">View Competent cells</a> | <a href="https://static.igem.org/mediawiki/2018/5/53/T--TecMonterrey_GDL--Protocols--1._Electro_competent_cells_protocol.pdf">View Competent cells</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/0/0c/T--TecMonterrey_GDL--Protocols--Digestion_and_ligation.pdf">View Procedure of digestion of the constructo</a> | <a href="https://static.igem.org/mediawiki/2018/0/0c/T--TecMonterrey_GDL--Protocols--Digestion_and_ligation.pdf">View Procedure of digestion of the constructo</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/3/34/T--TecMonterrey_GDL--Protocols-PCR.pdf">View PCR</a> | <a href="https://static.igem.org/mediawiki/2018/3/34/T--TecMonterrey_GDL--Protocols-PCR.pdf">View PCR</a> | ||
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</ul> | </ul> | ||
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<ul class="protocol-list"> | <ul class="protocol-list"> | ||
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<a href="https://static.igem.org/mediawiki/2018/d/d8/T--TecMonterrey_GDL--Protocols--2._Transformation_by_electroporation.pdf">View Transformation by electroporation</a> | <a href="https://static.igem.org/mediawiki/2018/d/d8/T--TecMonterrey_GDL--Protocols--2._Transformation_by_electroporation.pdf">View Transformation by electroporation</a> | ||
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</li> | </li> | ||
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<a href="https://static.igem.org/mediawiki/2018/7/75/T--TecMonterrey_GDL--Protocols--High_fidelity_restriction_enzymes_protocol.pdf">View High Fidelity restriction enzyme Protocol</a> | <a href="https://static.igem.org/mediawiki/2018/7/75/T--TecMonterrey_GDL--Protocols--High_fidelity_restriction_enzymes_protocol.pdf">View High Fidelity restriction enzyme Protocol</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/1/1d/T--TecMonterrey_GDL--Protocols--Miniprep.pdf">View MINIPREP KIT</a> | <a href="https://static.igem.org/mediawiki/2018/1/1d/T--TecMonterrey_GDL--Protocols--Miniprep.pdf">View MINIPREP KIT</a> | ||
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<ul class="protocol-list"> | <ul class="protocol-list"> | ||
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<a href="https://static.igem.org/mediawiki/2018/1/14/T--TecMonterrey_GDL--Protocols--3.1_Competent_cells_BL21.pdf">View Calcio competent cells</a> | <a href="https://static.igem.org/mediawiki/2018/1/14/T--TecMonterrey_GDL--Protocols--3.1_Competent_cells_BL21.pdf">View Calcio competent cells</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/6/65/T--TecMonterrey_GDL--Protocols--Protein_expression_with_T7_strains.pdf">View Protein Expression with T7 Strains</a> | <a href="https://static.igem.org/mediawiki/2018/6/65/T--TecMonterrey_GDL--Protocols--Protein_expression_with_T7_strains.pdf">View Protein Expression with T7 Strains</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/8/81/T--TecMonterrey_GDL--Protocols--1._Electrocompetent_cells_protocol_optimized_for_L._rhamnosus_GG.pdf">Electrocompetent cells protocol optimized for L. rhamnosus GG</a> | <a href="https://static.igem.org/mediawiki/2018/8/81/T--TecMonterrey_GDL--Protocols--1._Electrocompetent_cells_protocol_optimized_for_L._rhamnosus_GG.pdf">Electrocompetent cells protocol optimized for L. rhamnosus GG</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/2/21/T--TecMonterrey_GDL--Protocols--Plasmid_isolation_for_Lactobacillus_spp.pdf">View Plasmid isolation for Lacatobacillus spp.</a> | <a href="https://static.igem.org/mediawiki/2018/2/21/T--TecMonterrey_GDL--Protocols--Plasmid_isolation_for_Lactobacillus_spp.pdf">View Plasmid isolation for Lacatobacillus spp.</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/2/2a/T--TecMonterrey_GDL--Protocols--2._Electroporation_of_L._rhamnosus_GG.pdf">View Electroporation of L. rhamnosus GG.</a> | <a href="https://static.igem.org/mediawiki/2018/2/2a/T--TecMonterrey_GDL--Protocols--2._Electroporation_of_L._rhamnosus_GG.pdf">View Electroporation of L. rhamnosus GG.</a> | ||
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<a href="https://static.igem.org/mediawiki/2018/1/19/T--TecMonterrey_GDL--Protocols--Total_DNA_extraction_L._rhamnosus_GG_by_kit.pdf">View Total DNA extraction L. rhamnosus GG</a> | <a href="https://static.igem.org/mediawiki/2018/1/19/T--TecMonterrey_GDL--Protocols--Total_DNA_extraction_L._rhamnosus_GG_by_kit.pdf">View Total DNA extraction L. rhamnosus GG</a> | ||
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Revision as of 02:01, 18 October 2018