Line 4: | Line 4: | ||
<style> | <style> | ||
− | + | h1, | |
− | + | h2 { | |
− | + | color: #469789; | |
− | + | } | |
− | + | ||
− | + | .nav-pills .nav-link.active, | |
− | + | .nav-pills .show>.nav-link { | |
− | + | color: #fff !important; | |
− | + | background-color: #469789; | |
− | + | } | |
− | + | ||
− | + | #myScrollspy>nav>a { | |
− | + | color: #469789; | |
− | + | } | |
− | </style> | + | </style> |
− | + | ||
− | + | <body data-spy="scroll" data-target="#myScrollspy" data-offset="10"> | |
− | + | <div class="container-full "> | |
− | <div class="container-full " > | + | |
<div class="card"> | <div class="card"> | ||
<img class="card-img-top" src="https://static.igem.org/mediawiki/2018/4/47/T--SZU-China--demonstrate.png" /> | <img class="card-img-top" src="https://static.igem.org/mediawiki/2018/4/47/T--SZU-China--demonstrate.png" /> | ||
</div> | </div> | ||
</div> | </div> | ||
− | <div class="container-fluid" > | + | <div class="container-fluid"> |
<div class="row"> | <div class="row"> | ||
<div class="col-9 offset-1 shadow "> | <div class="col-9 offset-1 shadow "> | ||
Line 36: | Line 34: | ||
<div class="row"> | <div class="row"> | ||
<p> | <p> | ||
− | We wonder if the products made from recombinant Metarhizium anisopliae are convenient and useful | + | We wonder if the products made from recombinant <i>Metarhizium anisopliae</i> are convenient and useful. |
− | + | ||
</p> | </p> | ||
<p> | <p> | ||
− | + | Previously, emulsifiable powder as the product dosage form and non-woven fabric as the carrier suggested by Dr. Wang Zhongkang, can be proved that our product has stronger storage stability, UV resistance and thermal stability. Besides compared with spore spray, non-woven fabric use less material and small-scale use. | |
− | + | ||
</p> | </p> | ||
<p> | <p> | ||
− | + | For now, we have taken a step forward to verify our product has the functions including the ability of producing a great amount of spores, high mortality, and necessity of using GreenGround. | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</p> | </p> | ||
− | + | ||
</div> | </div> | ||
− | + | ||
− | <div class=" | + | <div id="Ability" class="indent"> |
+ | <h2> | ||
+ | Ability of producing a great amount of spores: | ||
+ | </h2> | ||
<p> | <p> | ||
− | In order to ensure that we can produce | + | In order to ensure that we can produce milk powder in large quantities, Professor Wang took us to visit the Chinese largest fungal pesticide factory. The production process is shown in the figure. |
− | + | ||
− | + | ||
</p> | </p> | ||
+ | |||
<div class="col-8 offset-2"> | <div class="col-8 offset-2"> | ||
<div class="card"> | <div class="card"> | ||
Line 71: | Line 68: | ||
<div class="row"> | <div class="row"> | ||
<p> | <p> | ||
− | It can be used after the emulsifiable powder is produced and brushed on the non-woven fabric | + | It can be used after the emulsifiable powder is produced and brushed on the non-woven fabric. |
</p> | </p> | ||
+ | </div> | ||
+ | |||
+ | <div id="mortality" class="indent"> | ||
+ | <h2>High mortality</h2> | ||
<p> | <p> | ||
− | + | We designed an experiment to investigate the difference between lethal period and mortality of our modified transformant and wild-type M.anisopliae. (According to the non-release principle, the experiment only tested the virulence of modified transformant and wild-type M.anisopliae in the laboratory.) | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
</p> | </p> | ||
+ | <p> | ||
+ | In group infected by wild-type M.anisopliae, we used 10μl emulsifiable powder in a dose of 10^9 conidia/mL to infects 10 cockroaches individually. And in group infected by modified transformant, we used the same among of powder to infect 10 cockroaches individually. | ||
+ | </p> | ||
+ | <p> | ||
+ | The results showed that wild-type M.anisopliae toke effect after two days, while transformant took effects less than one day. For mortality each day, transformant showed above two times significantly higher (P | ||
+ | <0.05) mortality than wild-type. Two groups reached stationary state after four days, above 20% mortality in wild-type, while 50% in transformant. </p> | ||
+ | </div> | ||
+ | |||
+ | <div class="col-10 offset-1"> | ||
+ | <div class="text-center"> | ||
+ | <caption> | ||
+ | Table 1: Experimental data of cockroaches infection experiment. * Table shows the number of cockroaches alive. | ||
+ | </caption> | ||
+ | </div> | ||
+ | <div class="card"> | ||
+ | <img class="card-img-top" src="https://static.igem.org/mediawiki/2018/1/14/T--SZU-China--Demo-0-1.png" /> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | |||
+ | <div class="row"> | ||
+ | |||
+ | <div class="col-8 offset-2"> | ||
+ | |||
+ | <div class="card"> | ||
+ | <img class="card-img-top" src="https://static.igem.org/mediawiki/2018/6/69/T--SZU-China--Demo-0-2.png" /> | ||
+ | </div> | ||
+ | <div class="text-center"> | ||
+ | <caption> | ||
+ | Fig 2: Mortality of cockroaches between wild type <i>M.anisopliae</i> and transformant. | ||
+ | </caption> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | </div> | ||
+ | <div class="row"> | ||
+ | <div class="col-4 offset-4"> | ||
+ | <div class="card"> | ||
+ | <img class="card-img-top" src="https://static.igem.org/mediawiki/2018/1/1c/T--SZU-China--cockroach.jpg" /> | ||
+ | |||
+ | </div> | ||
+ | <caption> | ||
+ | Fig 3:The cockroach which covered by spores | ||
+ | </caption> | ||
+ | </div> | ||
+ | </div> | ||
+ | |||
+ | <div id="Necessity" class="indent"> | ||
+ | <h2>Necessity of using GreenGround</h2> | ||
+ | <p> | ||
+ | We found some problems that the non-woven fabric is so light that it maybe fly away, and the emulsifiable powder exposure to light may die easier. | ||
+ | |||
+ | </p> | ||
+ | <p> | ||
+ | To solve this problem, we designed the GreenGround series, which has 3 generations of products (please click on Applied Design for details). We finally chose the third generation and patented it. In order to test the effect of the box on the killing effect, we did a comparative experiment and the results are as follows. | ||
+ | </p> | ||
+ | </div> | ||
+ | |||
+ | <div class="row"> | ||
+ | |||
<div class="col-8 offset-2"> | <div class="col-8 offset-2"> | ||
<table class="table text-center table-borderless"> | <table class="table text-center table-borderless"> | ||
Line 132: | Line 189: | ||
</div> | </div> | ||
<caption> | <caption> | ||
− | Fig | + | Fig 4: Mortality with or without box |
</caption> | </caption> | ||
</div> | </div> | ||
− | + | ||
</div> | </div> | ||
− | + | ||
<div class="row"> | <div class="row"> | ||
<p> | <p> | ||
− | This shows that our GreenGround can maintain the lethal effect of Metarhizium anisopliae, so it’s necessary to use. | + | This shows that our GreenGround can maintain the lethal effect of Metarhizium anisopliae, so it’s necessary to use. |
− | + | ||
</p> | </p> | ||
<div class="col-8 offset-2"> | <div class="col-8 offset-2"> | ||
Line 148: | Line 205: | ||
</div> | </div> | ||
<caption> | <caption> | ||
− | Fig | + | Fig 5:The third generation of GreenGround |
</caption> | </caption> | ||
− | + | ||
</div> | </div> | ||
− | + | ||
</div> | </div> | ||
+ | |||
<div class="row"> | <div class="row"> | ||
<p> | <p> | ||
− | + | Because of the Do Not Release Policy, we couldn’t test our transformant in our GreenGround. But from the evidence above we can see that, it has the ability of producing a great amount of spores, high mortality, and necessity of using GreenGround. We believe that if we combine our spores and GreenGround together, the product would work well. | |
− | + | ||
− | + | ||
</p> | </p> | ||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
− | |||
</div> | </div> | ||
− | + | ||
− | + | <div id="Reference" class="indent"> | |
− | <div | + | |
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
− | + | ||
<h2> | <h2> | ||
Reference: | Reference: | ||
Line 213: | Line 228: | ||
[1]Yin Youping, Wang Zhongkang, Xie Ning, et al. 2009. Insecticidal fungi emulsifiable powder:, CN101564047[P]. | [1]Yin Youping, Wang Zhongkang, Xie Ning, et al. 2009. Insecticidal fungi emulsifiable powder:, CN101564047[P]. | ||
</p> | </p> | ||
− | |||
− | |||
− | |||
</div> | </div> | ||
</div> | </div> | ||
− | |||
− | |||
<div class="col-2"> | <div class="col-2"> | ||
− | <nav id="myScrollspy" class="navbar | + | <nav id="myScrollspy" class="navbar position-fixed"> |
<nav class="nav nav-pills flex-column"> | <nav class="nav nav-pills flex-column"> | ||
− | + | <a class="nav-link" href="#Ability">Ability of producing spores</a> | |
− | <a class="nav-link" href="#"></a> | + | <a class="nav-link" href="#mortality">High mortality</a> |
+ | <a class="nav-link" href="#Necessity">Necessity of using GreenGround</a> | ||
+ | <a class="nav-link" href="#Reference">Reference</a> | ||
</nav> | </nav> | ||
Line 234: | Line 246: | ||
</div> | </div> | ||
− | + | </div> | |
</div> | </div> | ||
</body> | </body> | ||
+ | |||
+ | <script> | ||
+ | $('body').scrollspy({ | ||
+ | target: '#myScrollspy', | ||
+ | offset: 10 | ||
+ | }); | ||
+ | |||
+ | var scroll = new SmoothScroll('a[href*="#"]'); | ||
+ | </script> | ||
</html> | </html> |
Revision as of 02:29, 18 October 2018
Demonstrate
We wonder if the products made from recombinant Metarhizium anisopliae are convenient and useful.
Previously, emulsifiable powder as the product dosage form and non-woven fabric as the carrier suggested by Dr. Wang Zhongkang, can be proved that our product has stronger storage stability, UV resistance and thermal stability. Besides compared with spore spray, non-woven fabric use less material and small-scale use.
For now, we have taken a step forward to verify our product has the functions including the ability of producing a great amount of spores, high mortality, and necessity of using GreenGround.
Ability of producing a great amount of spores:
In order to ensure that we can produce milk powder in large quantities, Professor Wang took us to visit the Chinese largest fungal pesticide factory. The production process is shown in the figure.
It can be used after the emulsifiable powder is produced and brushed on the non-woven fabric.
High mortality
We designed an experiment to investigate the difference between lethal period and mortality of our modified transformant and wild-type M.anisopliae. (According to the non-release principle, the experiment only tested the virulence of modified transformant and wild-type M.anisopliae in the laboratory.)
In group infected by wild-type M.anisopliae, we used 10μl emulsifiable powder in a dose of 10^9 conidia/mL to infects 10 cockroaches individually. And in group infected by modified transformant, we used the same among of powder to infect 10 cockroaches individually.
The results showed that wild-type M.anisopliae toke effect after two days, while transformant took effects less than one day. For mortality each day, transformant showed above two times significantly higher (P <0.05) mortality than wild-type. Two groups reached stationary state after four days, above 20% mortality in wild-type, while 50% in transformant.
Necessity of using GreenGround
We found some problems that the non-woven fabric is so light that it maybe fly away, and the emulsifiable powder exposure to light may die easier.
To solve this problem, we designed the GreenGround series, which has 3 generations of products (please click on Applied Design for details). We finally chose the third generation and patented it. In order to test the effect of the box on the killing effect, we did a comparative experiment and the results are as follows.
With box | Without box | |
---|---|---|
Group 1 | 10 | 4 |
Group 2 | 9 | 2 |
Group 3 | 12 | 5 |
Mean | 9.5 | 3.0 |
Variance | 2.3 | 2.3 |
Mortality | 34.44% | 12.22% |
This shows that our GreenGround can maintain the lethal effect of Metarhizium anisopliae, so it’s necessary to use.
Because of the Do Not Release Policy, we couldn’t test our transformant in our GreenGround. But from the evidence above we can see that, it has the ability of producing a great amount of spores, high mortality, and necessity of using GreenGround. We believe that if we combine our spores and GreenGround together, the product would work well.
Reference:
[1]Yin Youping, Wang Zhongkang, Xie Ning, et al. 2009. Insecticidal fungi emulsifiable powder:, CN101564047[P].