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− | <p> | + | <p>In comparing Tsynth8 and Tsynth30 (Tmini) for use in yeast, both demonstrated increased heterologous protein expression and transcript levels greater than 2-fold over the commonly used TEF1 terminator [Curran et al.]. However, specifically for use in <i>Y. lipolytica</i>, Tsynth8 showed 3-fold greater protein expression than Tsynth30. In addition, Tsynth8 had slightly lower cryptic promoter activity and a lower tendency for transcript read-through, which would signal insufficient transcript termination. Most significantly, Tsynth8 could be ordered as a sequence from IDT; whereas Tsynth30, with it’s extended TA efficiency region, could not. |
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Revision as of 02:34, 18 October 2018
Improvement
In comparing Tsynth8 and Tsynth30 (Tmini) for use in yeast, both demonstrated increased heterologous protein expression and transcript levels greater than 2-fold over the commonly used TEF1 terminator [Curran et al.]. However, specifically for use in Y. lipolytica, Tsynth8 showed 3-fold greater protein expression than Tsynth30. In addition, Tsynth8 had slightly lower cryptic promoter activity and a lower tendency for transcript read-through, which would signal insufficient transcript termination. Most significantly, Tsynth8 could be ordered as a sequence from IDT; whereas Tsynth30, with it’s extended TA efficiency region, could not.
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