In comparing Tsynth8 and Tsynth30 (Tmini) for use in yeast, both demonstrated increased heterologous protein expression and transcript levels greater than 2-fold over the commonly used TEF1 terminator [Curran et al.]. However, specifically for use in Y. lipolytica, Tsynth8 showed 3-fold greater protein expression than Tsynth30. In addition, Tsynth8 had slightly lower cryptic promoter activity and a lower tendency for transcript read-through, which would signal insufficient transcript termination. Most significantly, Tsynth8 could be ordered as a sequence from IDT; whereas Tsynth30, with it’s extended TA efficiency region, could not.

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