Difference between revisions of "Team:TecMonterrey GDL/Parts"

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Latest revision as of 02:34, 18 October 2018

TecMonterrey_GDL

Parts

For E.coli

In the following table we present the parts we have used for our plasmid construction, detailed in Construct design, the parts we used are classified as they appear in our composite constructs:


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Strategy A : Part:BBa_K2760010 - Lactobachill Plan A (E. coli)


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Sub parts: BBa_K206000, BBa_J34801, BBa_K2760023, BBa_K2760024, BBa_B0015, BBa_K216005, BBa_K2760002, BBa_K2760012, BBa_K2760029










Strategy B: Part:BBa_K2760011 - Plan B for Lactobachill


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Sub parts: BBa_K206000, BBa_J34801, BBa_K2760023, BBa_K2760024, BBa_B0015, BBa_K216005, BBa_K2760003, BBa_K2760012, BBa_K2760029


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For L. rhamnosus GG


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Strategy A: Part: BBa_K2760033


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Sub parts: BBa_K2760005, BBa_K2760006, BBa_K2760023, BBa_K2760024, BBa_K2760007, BBa_K216005, BBa_K2760000,BBa_K2760002 BBa_K2760012, BBa_K2760029, BBa_K2760008
















Plan B (L. rhamnosus): Part: BBa_K276004


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Sub parts: BBa_K2760005, BBa_K2760006, BBa_K2760023, BBa_K2760006, BBa_K2760024, BBa_K2760007, BBa_K216005, BBa_K2760000, BBa_K2760003, BBa_K2760012, BBa_K2760029, BBa_K2760008


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Signaling peptides

For the excretion of recombinant proteins




For E.coli


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Sp1-Nsp4 construct: BBa_K2760019 - Characterization construct for SGP1-NSP4 -


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Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760000, BBa_K2760024, BBa_K2760012, BBa_B0015




BBa_K2760021 - SP2-Nsp2 Characterization construct


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Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760004, BBa_K2760024, BBa_K2760012, BBa_B0015


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BBa_K2760020 - Negative control for signal peptide characterization


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Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760024, BBa_K2760012, BBa_B0015




BBa_K2760022 - Positive Control for Characterization of Signal Peptides (DsbAss)


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Sub parts: BBa_I712074, BBa_Z0262, BBa_K2760001, BBa_K2760024, BBa_K2760012, BBa_B0015


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For L.rhamnosus


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Sp1-Nsp4- Characterization construct Part: BBa_K2760030


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Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760000, BBa_K2760029, BBa_K2760007









Sp2-Nsp2- Characterization Construct Part: BBa_K2760031


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Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760004, BBa_K2760029, BBa_K2760007


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Sp negative control construct: Part BBa_2760032


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Sub parts: BBa_K2760009, BBa_K2760005, BBa_K2760006, BBa_K2760029, BBa_K2760007

TecMonterrey_GDL