To confirm that the terminator Tsynth8 did in fact have more protein expression in Y. lipolytica, were replicated the experiment stated above. Tsynth8 should have 3-fold protein expression in Y. lipolytica. We inoculated Y. lipolytica with the terminator plasmids that had the gene hrGFP regulated by the following terminators: Tsynth8, Tsynth30, TEF1. These plasmids are the same exact plasmids that were used in the paper: Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast . Once inoculated, the cultures were diluted to accomplish the same OD600, and then every 2 hours their OD600 and fluorescence levels via nanodrop were taken. Our data for time versus OD600 can be seen in Fig.1, and the time vs fluorescence can be seen in Fig.2.

To confirm that the terminator Tsynth8 did have more protein expression in Y. lipolytica, we tried to replicate the experiment stated above. Where Tsynth8 should have 3-fold protein expression in Y. lipolytica. What we did is we inoculated Y. lipolytica with the terminator plasmids that had the gene hrGFP regulated by the following terminators: Tsynth8, Tsynth30, TEF1. These plasmids are the same exact plasmids that were used in the paper: Short Synthetic Terminators for Improved Heterologous Gene Expression in Yeast . Once inoculated, the cultures were diluted to the same OD600, and then every 2 hours their OD600 and fluorescence levels were taken. Our data for time versus OD600 can be seen in Fig.1, and the time vs fluorescence can be seen in Fig.2. This proves that Curren et al. paper is correct in that their is more protein expression but not at the 3 fold rate as he suggested.