Team:Goettingen/Attributions

Attributions

Outside of the team roster

RNA sequencing

For the RNA sequencing analyses, we asked Prof. Dr. Rolf Daniel and Dr. Anja Poehlein from the Göttingen Genomics Laboratory (G2L) for their support. The G2L delivered comprehensive RNA sequencing data that helped us to develop a glyphosate reporter system.

ITC measurements

For isothermal titration calorimetry (ITC) measurements and structure elucidation of the purified EPSP synthases from E. coli and B. subtilis we sent our proteins to the Bange lab in Marburg. Prof. Dr. Gert Bange is again supervising an iGEM team, which is acknowledged for collaborating.

DNA sequencing

The best thing that can happen to a DNA: to acquire DNA sequences, we sent our samples to the Microsynth Seqlab sequencing laboratories Microsynth Seqlab sequencing laboratories in Göttingen.

Analysis of DNA and genome sequences

We ordered some genes, called g-Blocks, from Integrated DNA Technologies, which was free of charge because the company supports the iGEM competition.

Detection of glyphosate

For the detection of glyphosate, we sent our samples to the lab from Dr. Till Ischebeck in the Department of Plant Biochemistry at the University of Göttingen. The samples were analyzed by GC/MS.

Genes

We ordered some genes, called g-Blocks, from Integrated DNA Technologies, which was free of charge because the company supports the iGEM competition.

Bacterial strains

We are grateful to Dr. Daniel R. Zeigler and Prof. Dr. John Helmann from the Bacillus Genetic Stock Center at The Ohio State University and the Helman lab at the Cornell University, respectively, for providing B. subtilis strains.

Contributions from the lab members

We thank Sabine Lentes for enduring eight people in the lab with many questions. She helped us in every situation and was a great aid.
Furthermore, we thank Johannes Gibhardt for introducing us to technical devices. He is an expert for the multi well plate readers!
Christina Herzberg supported us with strains and plasmids, knowledge about technical methods, and answered > 1000 questions.
Cedric Blötz helped us with his experience of being a former iGEM participant.
Anika Klewing supported us with buffers, kits and chemicals that we needed in the lab.
Last but not least, we want to thank all members from the Stülke lab for their support and endorsement during the time we used the lab and blocked technical devices with our experiments.

Work from team roster

We did all of the lab work by ourselfs as described in the notebook section. The experiments were developed in teamwork with our supervisors. The over all design was drafted by ourselves. Moreover, the wiki site and website were constructed on our own.

Supervisors

The whole project was stimulated by the ideas from PD Dr. Fabian M. Commichau that came up in his mind during a train ride to Brussels (that took not place). We want to thank him for his infectious enthusiasm for microbiology and his help to solve experimental problems!

We thank Prof. Dr. Jörg Stülke for kindly providing us with lab space, which is essential for a research project. Furthermore, he greatly supported us with funding and his experience as a leader of the iGEM team of the University Göttingen in 2013.

Construction of strains

We are grateful to Sabine Lentes and PD. Dr. Fabian M. Commichau for the construction of B. subtilis mutants.