Team:CCU Taiwan/Judge

Background

All of the 2018 iGEM teams are invited and encouraged to participate in the Fifth International InterLaboratory Measurement Study in synthetic biology.” Our team took part in this study which aimed to be one of the labs to make the calibration in different condition. In our case, we measured fluorescence using plate reader.

Interlab Project

OD₆₀₀ Reference point - LUDOX Protocol

Particle Standard Curve - Microsphere Protocol

Fluorescence standard curve - Fluorescein Protoco

Cell measurement protocol

Colony Forming Units per 0.1 OD₆₀₀ E. coli cultures

Experiment

Calibration 1: OD₆₀₀ Reference Point - LUDOX Protocol

Materials：

1. 1ml LUDOX CL-X (provided in kit)
2. dd H₂O (provided by team)
3. 96 well plate, black with clear flat bottom preferred (provided by team)