Team:UCopenhagen/Notebook
Lab notebook
The notebook is a chronological overview of what has happened in the lab. It is organized after each experiment.Cloning
Making BioBricks
Liposome characterization
Onion characterization
Leakiness characterization
Membranes and microscope
Week 28
Learning to make liposomes – 12/07/18
We were introduced to the process of making liposomes by the nice people in Nikos Hatzakis lab, Søren Schmidt-Rasmussen Nielsen and Mette Galsgaard Malle who gave us the Preparing liposomes protocol. We made some tryout liposomes, that were flashed freezed and stored in -20 oC.
Selma, Sofia and AttilaWeek 34
Transforming SIEC strains with GFP plasmids – 24/08/18
To visualize our bacteria in the microscope together with a membrane we needed it transformed with a GFP plasmid with constitutive expression. We used plasmids from the distribution kit that functioned as Test Device 1 (BBa_J36400) and positive control (BBa_I20270) in the InterLab study. Transformation as described in Transformation protocol until step 14, the following combinations of strain and plasmid was made:
- SIEC x BBa_J36400
- SIEC x BBa_I20270
- SIECΔp1 x BBa_J36400
- SIECΔp1 x BBa_I20270
Plates were made from agar and LB with chloramphenicol (34 μg/mL) (CAM). Plates with bacteria spent 17 hours in the incubator before being kept in fridge. Conclusion: hereafter we used the bacteria transformed with BBa_I20270.
Selma