Team:Goettingen/Collaborations

Collaborations

Short Summary

IGEM is an international competition that promotes the search for solutions of problems in the world using synthetic biology. While every team is working on their own projects in their local laboratories utilizing the biobricks provided by iGEM over the summer, they design new parts to add to the collection of biobricks at the end of the competiton. This makes every team part of a big community, that works togehter to promote the transparent and innovative developement of new tools for engineering biology. This community is not only about the collection of biobricks, but also about helping each other. Collaborations of individual teams are one of the most important aspects of iGEM, where teams help each other achieve their goals. This can be just by giving helpful adivse or even doing experiments together. We collaborated intensively with the team of Marburg to determine the binding affinity of AroE and AroA with glyphosate.

Collaboration - Biochemical characterization of EPSP synthase in cooperation with iGEM Marburg

This year, our team came toghether with the iGEM team of Marburg, to investigate the interaction of the EPSP synthases AroE and AroA with glyphosate. AroE is the target of glyphoasate in B. subtilis and AroA is the target in E. coli. Since the university of Marburg is known for their skills in the field of Isothermal titration calorimetry (ITC), we did not hesitate to contact them for support. Our team constructed strains which allowed the overexpression of the proteins of interest fused to a Strep-tag. We then cultivated the cells and induced the overexpression of either AroA or AroE. Afterwards we purified the proteins and send them to the iGEM team in Marburg. (Bild purification) They performed the ITC measurments