Biosynthesis of CdS semiconductor
To construct our light-driven system, we first induce the precipitation of CdS semiconductor on the cell membrane. Two plasmids encoding the surface display protein OmpA-PbrR and the nitrogenase are co-transferred into E. coli strain. After Cd2+ is added into the media, the ions specifically bind to PbrR leading to aggregation of Cd2+ ions. At last when S2- ions are added into the media, E. coli cells for