Team:Goettingen/Parts
Team Göttingen
iGEM 2018
Glyphosate on my plate?
| E-mail: | igem2018@gwdg.de |
|---|---|
| Adress: | c/o iGEM 2018 |
| Department of General Microbiology | |
| Grisebachstraße 8 | |
| 37077 Göttingen | |
| Germany |
We are especially grateful to our numerous sponsors on gofundme, who supported our project and made it possible.
Every public donor, who helped us to achieve our goals got a special place on our bacterial wall of fame and a handdrawn microbe to show our appreciation. A link to that wall can be found here:
Parts
Connecting global research
Our contribution to a huge parts collection.
The concept of iGEM is based on the exchange of internationally provided DNA sequences. These sequences are called Biobricks and are collected in a big database, which grows steadily as iGEM progresses. In this way, teams all over the globe can benefit from the part collection and improve the work that was done previously by other teams, to drive the research further.
Basic parts and composite parts
Basic parts are functional DNA units that cannot be divided into smaller parts. A construct of multiple basic parts is called a composite part. Here, the functionality of a basic part was increased through the implementation of different functional sites. The parts of our team are listed and shortly described in the following table:
| Part Number and Name | Short Description | Type | Length in [bp] |
|---|---|---|---|
| BBa_K2586000/Palf4 | Promoter for gltT expression | Promoter | 30 |
| BBa_K2586001/gltT | Uptake of glutamate from the environment | Coding | 1290 |
| BBa_K2586002/gltP | Uptake of glutamate from the environment | Coding | 1245 |
| BBa_K2586003/aroE | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate | DNA | 1287 |
| BBa_K2586004/Strep-Tag-aroE | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate in Bacillus subtilis | Tag | 1410 |
| BBa_K2586005/PtrpP | Promoter for trpP in Bacillus subtilis | Promoter | 467 |
| BBa_K2586006/Strep-Tag-aroA | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate in Escherichia coli | Tag | 1407 |
| BBa_K2586007/aroA | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate | DNA | 1284 |
| BBa_K2586008/RBS | Binding site for ribosome | Binding Site | 13 |
| BBa_K2586009/RBS-gltT | Ribosome binding site and glutamate transporter | Composite | 1303 |
| BBa_K2586010/Palf4-RBS | Promoter and Ribosome binding site | Composite | 94 |
| BBa_K2586011/Palf4-RBS-gltT | Promoter and Ribosome binding site for expresssion of glutamate transporter | Composite | 1347 |
| BBa_K2586012/Palf4-RBS-GAT | yet to come | Composite | 498 |
| BBa_K2586013/Palf4-RBS-AroA* | yet to come | Composite | 1341 |
| BBa_K2586019/GAT | Acetylation and therefore inactivation of glyphosate | Enzyme | 440 |
| BBa_K2586020/AroA* | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate | DNA | 1283 |
Furthermore, we characterized an existing part. We chose BBa_E2050 (mERP), wich we transformed into different Bacillus strains. Because the plasmid pSB1C3 does not contain an origin of replication for Bacillus, we cloned the fluorophore into the plasmid pAC7 and transformed it into competent DH5α. The fluorophore was additionally coupled to a self-made promoter, which is characterized by a perfect consensus sequence and perfect RBS for Bacillus. Further information can be found on the Registry. Additionally, we used the strains for further experiments (check out the Results section).