Team:Goettingen/Notebook
Team Göttingen
iGEM 2018
Glyphosate on my plate?
| E-mail: | igem2018@gwdg.de |
|---|---|
| Adress: | c/o iGEM 2018 |
| Department of General Microbiology | |
| Grisebachstraße 8 | |
| 37077 Göttingen | |
| Germany |
We are especially grateful to our numerous sponsors on gofundme, who supported our project and made it possible.
Every public donor, who helped us to achieve our goals got a special place on our bacterial wall of fame and a handdrawn microbe to show our appreciation. A link to that wall can be found here:
Our Labnotes:
STRAIN LIST
| Name | Name after isolation |
|
|---|---|---|
| 168 | trpC2 | |
| SP1 | wildtype | |
| iGEM1 | Suppressor 1 10 mM | trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT |
| iGEM2 | Suppressor 2 10 mM | trpC2 not determined |
| iGEM3 | Suppressor 3 10 mM | trpC2 not determined |
| iGEM4 | Suppressor 4 10 mM | trpC2 not determined |
| iGEM5 | Suppressor 5 10 mM | trpC2 not determined |
| iGEM6 | Suppressor 1-1 30 mM | trpC2 not determined |
| iGEM7 | Suppressor 1-2 30 mM | trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT gltP(G232A) |
| iGEM8 | Suppressor 2-1 30 mM | trpC2 not determined |
| iGEM9 | Suppressor 2-2 30 mM | trpC2 not determined |
| iGEM10 | Suppressor 3-1 30 mM | trpC2 not determined |
| iGEM11 | Suppressor 3-2 30 mM | trpC2 not determined |
| iGEM12 | Suppressor 1-1 40 mM | trpC2 not determined |
| iGEM13 | Suppressor 1-2 40 mM | trpC2 1.3 kbp deletion 5'-yhfE-yhfF-3'-gltT 11 bp deletion in gltP |
| iGEM14 | Suppressor 2-1 40 mM | trpC2 not determined |
| iGEM15 | Suppressor 2-2 40 mM | trpC2 not determined |
| iGEM16 | Suppressor 3-1 40 mM | trpC2 not determined |
| iGEM17 | Suppressor 3-2 40 mM | trpC2 not determined |
| BP233 | trpC2 ΔgltT::spc | |
| BP234 | trpC2 ΔgltP::cat | |
| BP235 | trpC2 ΔgltT::spc ΔgltP::cat | |
| BP236 | trpC2 amyE::(Palf4-gltT-lacZ aphA3) | |
| BP237 | trpC2 ΔgltT::spc amyE::(Palf4-gltT-lacZ aphA3) | |
| iGEM18 | amyE::(PtrpE-lacZ aphA3) | |
| iGEM19 | trpC2 amyE::(PtrpE-lacZ aphA3) | |
| iGEM20 | SP1_GP342 | amyE::(PgltA-lacZ aphA3) |
| iGEM21 | SP1_GP650 | amyE::(PgltA-lacZ aphA3) gltC::TnIO spc |
| iGEM22 | 168::BFP | trpC2 amyE::(BFP-lacZ aphA3) |
| iGEM23 | 168::GFP | trpC2 amyE::(GFP-lacZ aphA3) |
| iGEM24 | 168::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc |
| iGEM25 | 168::mCherry | trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc |
| iGEM26 | BP233::BFP | trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc |
| iGEM27 | BP233::GFP | trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc |
| iGEM28 | BP233::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc |
| iGEM29 | BP233::mCherry | trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc |
| iGEM30 | Spden::BFP | trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM31 | Spden::GFP | trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM32 | Spden::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM33 | Spden::mCherry | trpC2 amyE::(mCherry-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM34 | BP235::BFP | trpC2 amyE::(BFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM35 | BP235::GFP | trpC2 amyE::(GFP-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM36 | BP235::mOrange | trpC2 amyE::(mOrange-lacZ aphA3) ΔgltT::spc ΔgltP::cat |
| iGEM37 | SP1::Gat | amyE::(gat-lacZ aphA3) |
| iGEM38 | SP1::AroA* | amyE::(aroA*-lacZ aphA3) |
| iGEM39 | SP1::Gat::ΔgltT | amyE::(gat-lacZ aphA3) ΔgltT::spc |
| iGEM40 | SP1::AroA*::ΔgltT | amyE::(aroA*-lacZ aphA3) ΔgltT::spc |
| iGEM41 | SP1::ΔgltT | ΔgltT::spc |