Team:Goettingen/Parts

Parts

Connecting global research

Our contribution to a huge parts collection.

The concept of iGEM is based on the exchange of internationally provided DNA sequences. These sequences are called Biobricks and are collected in a big database, which grows steadily as iGEM progresses. In this way, teams all over the globe can benefit from the part collection and improve the work that was done previously by other teams, to drive the research further.

Basic parts and composite parts

Basic parts are functional DNA units that cannot be divided into smaller parts. A construct of multiple basic parts is called a composite part. Here, the functionality of a basic part was increased through the implementation of different functional sites. The parts of our team are listed and shortly described in the following table:

Part Number and Name
Short Description
Type Length [bp]
BBa_K2586000/Palf4 artificial promoter Promoter 30
BBa_K2586001/gltT Uptake of glutamate from the environment Coding 1290
BBa_K2586002/gltP Uptake of glutamate from the environment Coding 1245
BBa_K2586003/aroE Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate Coding 1287
BBa_K2586005/PtrpP Promoter for PtrpP in Bacillus subtilis Promoter 470
BBa_K2586007/aroA Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate Coding 1290
BBa_K2586008/RBS Ribosome binding site Regulatory 13
BBa_K2586010/Palf4-RBS Promoter connected to RBS Composite 94
BBa_K2586019/gat Glyphosate N-acetyl-transferase Coding 480
BBa_K2586020/aroA* Mutated aroA Coding 480
BBa_K2586021/RBS-gltT RBS connected to gltT Composite 1355

Furthermore, we characterized an existing part. We chose BBa_E2050 (mERP), wich we transformed into different Bacillus strains. Because the plasmid pSB1C3 does not contain an origin of replication for Bacillus, we cloned the fluorophore into the plasmid pAC7 and transformed it into competent DH5α. The fluorophore was additionally coupled to a self-made promoter, which is characterized by a perfect consensus sequence and perfect RBS for Bacillus. Further information can be found on the Registry. Additionally, we used the strains for further experiments (check out the Results section).

<groupparts>"iGEM018" "Goettingen"</groupparts>