Team:Goettingen/Part Collection
Team Göttingen
iGEM 2018
Glyphosate on my plate?
| E-mail: | igem2018@gwdg.de |
|---|---|
| Adress: | c/o iGEM 2018 |
| Department of General Microbiology | |
| Grisebachstraße 8 | |
| 37077 Göttingen | |
| Germany |
We are especially grateful to our numerous sponsors on gofundme, who supported our project and made it possible.
Every public donor, who helped us to achieve our goals got a special place on our bacterial wall of fame and a handdrawn microbe to show our appreciation. A link to that wall can be found here:
Part collection for glyphosate resistance
In this collection, we gathered all parts that contribute to the resistance against the herbicide glyphosate. First of all the two newly identified glyphosate transporters GltT and GltP. If both transporters are not present anymore, the soil-bacterium Bacillus subtilis is glyphosate-resitant. The target of glyphosate is the EPSP-synthase, which is encoded by aroE in Bacillus subtilis and by aroA in Escherichia coli. If the target is mutated, like in our part BBa_K2586020, the cells are characterized by a high glyphosate-resistance. If the cells express the gene for the Glyphosate-N-acetyl-transferase (GAT), they are also resistant. In conclusion, this collection provides three different resistance modes.
| Part Number and Name | Type | Length [bp] | |
|---|---|---|---|
| BBa_K2586001/gltT | Uptake of glutamate from the environment | Coding | 1290 |
| BBa_K2586002/gltP | Uptake of glutamate from the environment | Coding | 1245 |
| BBa_K2586003/aroE | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate | Coding | 1287 |
| BBa_K2586007/aroA | Converts Shikimate-3-phosphate into 5-enolpyruvylshikimate-3-phosphate | Coding | 1290 |
| BBa_K2586019/gat | Glyphosate N-acetyl-transferase | Coding | 480 |
| BBa_K2586020/aroA* | Mutated aroA | Coding | 480 |