Zhao Wenxue
Team Captain & Lab Manager
Team:SKLMT-China/Notebook Protocol
Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.
Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.
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Description
Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.
Pseudomonas fluorescens Pf-5(pf5) is the only safety bacteria using in Chinese agriculture approved by Ministry of agricultural and rural affair of China . Besides ,this strain has been confirmed to be non-pathogenic to mammals. Compared with E.coli, the developed organisms , the toolkit for Pseudomonas fluorescens seems not exploit well. We are going to build a promoter library of inner promoters with various strength in pf5. We characterize the strength by a fluorescent reporter gene, firefly. An additional vector is that We hope to engineer our bacteria with the nicotine degradation gene cluster from Agrobacterium S33 into Pseudomonas fluorescens Pf-5 by rec/ET recombination , so that Pseudomonas fluorescens Pf-5 could degrade nicotine. These bacteria will be used to handle nicotine in cigarette butts or in the environment. At the same time, we would apply the promoter library to control nicotine degradation efficiency.
Another
| Component | Amount(ul) | Final |
|---|---|---|
| Autoclaved ddH2O | 22 | |
| 2×primeSTAR max premix | 25 | 1× |
| Template, 50ng ul-1 | 1 | 50 ng |
| Syn-promoter-3 Oligo(10uM) | 1 | 30 pmol |
| promoter-4 Oligo(10uM) | 1 | 30 pmol |
| Total | 50 |
Members
Here are our team members.
Zhao Xiaohan
Team Captain & Lab Manager
Tang Fei
Team Captain & Lab Manager
Tian Weixuan
Lab Manager & HP Manager
Dong Dongyang
Data Manager & Lab Manager
Sun Ao
Lab Manager
Liang Xueyuan
Communication Manager & Finance Manager
Li Hanzhen
Designer & Competition Affairs
Shan Hongkun
Human Practice Manager
Chen Nuo
Modeling Manager
Gao Dechen
Wiki Manager