Team:SKLMT-China/Notebook Overview
| MasterMix | ||
|---|---|---|
| Cut Smart buffer | (n + 1) x | 0.2 µl |
| PvuII-HF | (n + 1) x | 0.2 µl |
| dH2O | (n + 1) x | 4.8 |
| Total volume | (n + 1) x | 6.0 µl |
• Add 6.0 µl of the Mastermix to 4.0 µl of the dissolved plasmid DNA
• Incubate for at least 1.5 h at 37°C.
• Load the whole mixture with loading buffer on a 0.7% agarose gel (containing EtBr) and perform electrophoresis. Take a picture.