Team:SKLMT-China/Model Process

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Background

We have built a promoter library of inner promoters with various strength in pf5. We characterized the strength by a fluorescent reporter gene, firefly luciferase. Having gotten so many statistics, we thought about determine element for the strength of promoters. As we all know, strength composite promoters usually have high affinity with RNA polymerase related to the UP elements like −35 sequences and −10 sequences. We wonder the connection between the promoter sequence and its strength. So we use the resulting fluorescence data to construct a model with the promoter sequence.

·To facilitate the promoter transformation of pseudomonas fluorescence in new site;

·To reveal the correlation between promoter weights and their strength;

·To improve the parameter setting of the promoter strength prediction in the species outside the large intestine with the method of position weight;

·To provide reference for other microbe promoter strength prediction modeling.

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