Team:SZU-China/Demonstrate

Demonstrate

We designed an experiment to investigate the difference between lethal period and mortality of our modified transformant and wild type M.anisopliae.

In group infected by wild type M.anisopliae, we used 10μl emulsifiable powder in dose of 109 conidia/mL to infects 17 cockroaches individually. And in group infected by modified transformant, we used the same among of powder to infect 10 cockroaches individually.

The results showed that wild type M.anisopliae toke effect after two days, while transformant took effects less than one day. For mortality each day, transformant showed above two times significantly higher (P <0.05) mortality than wild type. Two groups reached stationary state after four days, above 20% mortality in wild type, while 50% in transformant.

Table 1: Experimental data of cockroaches infection experiment. * Table shows the number of cockroaches alive.
Figure 1: Mortality of cockroaches between wild type M.anisopliae and transformant.

We consulted the relevant literature and found that the M.anisopliae emulsifiable powder emulsion is easy to fail under the sunlight, so we designed a box that can hold emulsifiable powder, called “Green Ground”. In order to verify the validity of the box(final generation), we designed an experiment, we first applied the emulsifiable powder to two non-woven fabrics, one in the box, we named it “With Box”, the other in the environment which called “Without Box”, and put an equal amount of cockroaches. After three days, the number of dead cockroaches in the two groups was counted. Repeated three times, the average mortality rate of the two groups was calculated and compared, and the mortality rate of the With Box group was higher. (As the chart shows)

With box Without box
Group 1 10 4
Group 2 9 2
Group 3 12 5
Mean 9.5 3.0
Variance 2.3 2.3
Mortality 34.44% 12.22%
Figure 2: Mortality of cockroaches using or without using our box.