Team:SZU-China/Demonstrate
Demonstrate
We designed an experiment to investigate the difference between lethal period and mortality of our modified transformant and wild type M.anisopliae.
In group infected by wild type M.anisopliae, we used 10μl emulsifiable powder in dose of 109 conidia/mL to infects 17 cockroaches individually. And in group infected by modified transformant, we used the same among of powder to infect 10 cockroaches individually.
The results showed that wild type M.anisopliae toke effect after two days, while transformant took effects less than one day. For mortality each day, transformant showed above two times significantly higher (P <0.05) mortality than wild type. Two groups reached stationary state after four days, above 20% mortality in wild type, while 50% in transformant.
We consulted the relevant literature and found that the Metarhizium emulsifiable powder emulsion is easy to fail under the sunlight, so we designed a box that can hold emulsifiable powder, called “Green Ground”. In order to verify the validity of the box(final generation), we designed an experiment, we first applied the emulsifiable powder to two non-woven fabrics, one in the box, we named it “With Box”, the other in the environment which called “Without Box”, and put an equal amount of cockroaches. After three days, the number of dead cockroaches in the two groups was counted. Repeated three times, the average mortality rate of the two groups was calculated and compared, and the mortality rate of the With Box group was higher. (As the chart shows)
| With box | Without box | |
|---|---|---|
| Group 1 | 10 | 4 |
| Group 2 | 9 | 2 |
| Group 3 | 12 | 5 |
| Mean | 9.5 | 3.0 |
| Variance | 2.3 | 2.3 |
| Mortality | 34.44% | 12.22% |
To ensure that we can produce spore powder in large quantities, we visited Professor Wang Zhongkang, who told us about the fermentation production and took us to visit the factory, which provided conditions for us to produce products in the future.
Fermentation production process as shown：
We used a series of produced emulsifiable powder in combination with our designed boxes to carry out a series of simulation experiments. According to Professor Wang Zhongkang's suggestion, we chose non-woven fabric as the carrier of the emulsifiable powder, because the emulsifiable powder can be attached to the non-woven fabric in a large amount, which greatly increases the probability of infection.
After producing the emulsion, we applied it to the non-woven cloth and placed the non-woven cloth in area A of the box. Then screw the lid down as shown in the pic through the cylinder to form crevices which are also adjustable. The cockroaches can climb through the slope B and get in contact with Metarhizium anisopliae which will lead to death.
We recommend changing a pack of emulsifiable powder every week.
