Team:TecMonterrey GDL/Protocols

TecMonterrey_GDL

Protocols

For E.coli :

View Preparation of LB Medium

View LB medium and LB agar-plates Protocol

View Competent cells

View Transformation by electroporation

View Calcio competent cells

View Ligation and digestion of G-Blocks

View Transformation Heat Shock

View MINIPREP KIT

View PCR

View Agarose Gel Electrophoresis

View Optimizing Restriction Endonuclease Reactions

View Protein extraction and purification based on Thermo Fisher's Protocol

View SDS-PAGE

View Western Blot based on Invitrogen's protocol

For Peptides :

View Competent cells

View Transformation by electroporation

View Calcio competent cells

View Procedure of digestion of the constructo

View High Fidelity restriction enzyme Protocol

View MINIPREP KIT

View PCR

View Protein Expression with T7 Strains

For L. rhamnosus GG :

Electrocompetent cells protocol optimized for L. rhamnosus GG

View Electroporation of L. rhamnosus GG.

View Plasmid isolation for Lacatobacillus spp.

View Total DNA extraction L. rhamnosus GG