Team:NTHU Formosa/Interlab

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IGEM 2018 Interlab Study

Overall To-Do List

Calibration 1：​​ Reference point - LUDOX Protocol Record settings for standard measurements Record measurements Fill out corresponding Excel sheet Calibration 2：​Particle Standard Curve - Microsphere Protocol Prepare Microsphere Stock Solution Serial dilution Measure Abs600 of all samples in instrument Record measurements Fill out corresponding Excel sheet Calibration 3：Fluorescence standard curve - Fluorescein Protocol Resuspend and dilute fluorescein Serial dilutions Measure fluorescence of all samples in instrument Record measurements Fill out corresponding Excel sheet Cell Measurement protocol Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3) Day 2: pick 2 colonies per plate (5 mL LB medium + Chloramphenicol. Grow 16-18 hours @ 37 C and 220 rpm Day 3: Dilution cultures in LB+Chloramphenicol Measure Further dilute Incubate Abs600 and fluorescence measurement Record measurements Fill out corresponding Excel sheet Protocol：Colony Forming Units per 0.1 E. coli cultures

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