Team:NTHU Formosa/InterLab

2018 iGEM Interlab Study

Overall To-Do List

✔ Calibration 1：OD₆₀₀ Reference point - LUDOX Protocol

1.Record settings for standard measurements

2.Record measurements

3.Fill out corresponding Excel sheet

✔ Calibration 2：Particle Standard Curve - Microsphere Protocol

1.Prepare Microsphere Stock Solution

2.Serial dilution

3.Measure Abs600 of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet

✔ Calibration 3：Fluorescence standard curve - Fluorescein Protocol

1.Resuspend and dilute fluorescein

2.Serial dilutions

3.Measure fluorescence of all samples in instrument

4.Record measurements

5.Fill out corresponding Excel sheet

✔ Cell Measurement protocol

Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3)

Day 2: Pick 2 colonies per plate (5 mL LB medium + Chloramphenicol.

Grow 16-18 hours @ 37 C and 220 rpm

Day 3: Dilution cultures in LB+Chloramphenicol

Measure OD₆₀₀

Further dilute

Incubate

Abs600 and fluorescence measurement

Record measurements

Fill out corresponding Excel sheet

✔ Protocol：Colony Forming Units per 0.1 OD₆₀₀ E.coli cultures

✔ To see the results, click here.