Measurement of Abs 600 and OD 600 for LUDOX CL-X and water
Preparation of a dilution series of silica microspheres and measurement of the Abs 600 in a plate reader
Preparation of a dilution series of fluorescein in four replicates and measurement of the fluorescence
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Transformation of E. coli DH5α with InterLab study plasmids
Picking colonies from the transformed plates and inoculation in 5 mL LB medium + Chloramphenicol and overnight culture
Sampling and assay
Triplication of the same procedures to confirm the reproducibility
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A-tailing process at the end of our gene fragment for TA cloning
Ligation of the fragment with TA vector
Transformation of DH5 α E. coli with the ligated vector (Blue-white screen)
Picking colonies from the plates and overnight culture with 0.1 mM of ampicillin
Isolation of the amplified plasmid using DNA mini prep kit
Purification of the DNA fragment gel electrophoresis and ligation with pSB1C3.
Verification of appropriate insertion of the construct in the vector
Duplication of the same procedures for debugging
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