Team:LZU-CHINA/Notebook
2017
Month
What we have done
June
Design IGEM engineering scheme.
July and August
Explore the culture conditions of different gastric cancer cell lines.
September, October
Design IGEM engineering scheme.
November
Lentiviral vector construction and partial parts synthesis.
2018
Month
What we have done
February
Obtain stable cell line of miR attacker.
March
Detect the effect of micro RNA on gastric cancer cell line through scratch test and CCK-8 experiment.
April
Detect the effect of microRNA on cell cycle of gastric cancer cell lines by flow cytometry.
May
Get stable cell line of Exosome booster.
June
Exosomes were isolated and identified by WB and electron microscopy.
July
NTA analysis validates Exosome booster’s function.
August
Confocal microscopy was used to verify exosomes uptake.
September
Build IGEM parts and submit to IGEM headquarters.
October
Detect the transcription efficiency of induced promoters under different inductors. And, Giant Jamboree~