Team:Munich/Protocols

Protocols

Cloning

Colony PCR

Chemical transformation

Agarose-Gel electrophoresis

Electrocompetent transformation

Ligation

PCR

Restriction digest

Sequencing sample preparation

Double Layer Photolitography

Oracoli Microscopy

Soft Lithography

Genome Engineering

UV-Vis (Mexico)

Chemical transformation

Agarose-Gel electrophoresis

pRED/ET Engineering

Cell-Free System

Transformation

Buffer and Media Preparation

Cell Cultivation(Shaking Flask method)

Cell-Cultivation(Fermentation)

Harvesting and Washing

Sonication

Bead beating

Dialysis

BCA Assay

TX-TL

DNA Processing

Digestion

Phages

Agar overlay plaque assay

Alginate encapsulation

Arbor TX/TL

Burst size analysis (1:10 Transfection)

Burst size analysis (Heinemann, 2007)

cDNA Synthesis and RNase treatment

DNA Cleanup Kit

Optimized Alginate Encapsulation

Phage T7 Assembly TX/TL

Phage T7 DNA Purification

Phenol-Chloroform precipitation

Preparation of Urea gel

RNA Extraction

Quantitative PCR

RNA Extraction with DNase treatment

Ultrazentrifugation

Protein Purification

Lyophilization

Ultrazentrifugation

Phages in SGF SIF

Phage Purifier

Microspheres in Acid

Cpf1

Western Blot

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