Team:NAU-CHINA/Part Collection
PARTS
Part Collection
Our Best Composite Part:BBa_K2557010
TetO-miniCMV promoter-Bxb1(BBa_K2557010) is composed of TetO(BBa_K2557038), miniCMV promoter(BBa_K2557032) and Bxb1 recombinase(BBa_K2557001). It is regulated by TetR and can express Bxb1 recombinase to open the expression of RFP. Bxb1 attB-RFP-Bxb1 attP(BBa_K2557002) consist of Bxb1 attB(BBa_K2557041), tagRFP(BBa_K2557035) and Bxb1 attP(BBa_K2557042), functioning as reporting part. miniCMV promoter-Bxb1-RDF(BBa_K2557019) is composed of miniCMV promoter(BBa_K2557032) and Bxb1-RDF(BBa_K2557047), which expresses Bxb1-RDF fusion protein to block the expression of RFP. TetO-miniCMV promoter-Bxb1(BBa_K2557010), miniCMV promoter-Bxb1-RDF(BBa_K2557019) and Bxb1 attB-RFP-Bxb1 attP(BBa_K2557002), three of them ensure the uniqueness of downstream output.
Part Collection
| Name | Type | Description | Length |
|---|---|---|---|
| BBa_K2557001 | Coding | Bxb1 recombinase | 1565 |
| BBa_K2557002 | Composite | Bxb1 attB-RFP-Bxb1 attP | 935 |
| BBa_K2557010 | Composite | TetO-miniCMV promoter-Bxb1 | 1890 |
| BBa_K2557019 | Composite | miniCMV promoter-Bxb1-RDF | 2391 |
| BBa_K2557032 | promoter | miniCMV promoter | 38 |
| BBa_K2557035 | Reporter | TagRFP | 711 |
| BBa_K2557038 | DNA | TetO | 271 |
| BBa_K2557041 | DNA | Bxb1 attB | 96 |
| BBa_K2557042 | DNAe | Bxb1 attP | 112 |
| BBa_K2557047 | Coding | Bxb1-RDF | 2345 |
Nanjing Agricultural University
College of Life Sciences of
Nanjing Agricultural University
Nanjing University
Chinese Academy of Sciences
