Team:NTHU Formosa/Interlab

IGEM 2018 Interlab Study

Overall To-Do List

Calibration 1:​​ Reference point - LUDOX Protocol Record settings for standard measurements Record measurements Fill out corresponding Excel sheet Calibration 2:​Particle Standard Curve - Microsphere Protocol Prepare Microsphere Stock Solution Serial dilution Measure Abs600 of all samples in instrument Record measurements Fill out corresponding Excel sheet Calibration 3:Fluorescence standard curve - Fluorescein Protocol Resuspend and dilute fluorescein Serial dilutions Measure fluorescence of all samples in instrument Record measurements Fill out corresponding Excel sheet Cell Measurement protocol Day 1: Transform E.Coli DH5α with plasmids (all in pSB1C3) Day 2: pick 2 colonies per plate (5 mL LB medium + Chloramphenicol. Grow 16-18 hours @ 37 C and 220 rpm Day 3: Dilution cultures in LB+Chloramphenicol Measure Further dilute Incubate Abs600 and fluorescence measurement Record measurements Fill out corresponding Excel sheet Protocol:Colony Forming Units per 0.1 E. coli cultures