Team:USMA-West Point/Notebook
Notebook
May 2018
- Presentation of overall project to develop cell-based artificial chemosensory detectors at MIT Solider Design Competition
June 2018
- Started design for synthetic operon plasmid
July 2018
- Submitted design for synthetic operon plasmid to GeneWiz for synthesis
- Interlab studies completed
August 2018
- Designed primers for hOR1D2 expression plasmid
- Amplified hOR1D2 open reading frame by PCR
September 2018
- Established collaboration with USAFA iGEM team
- Started analysis of USAFA iGEM plasmid intended for DNA part submission
- Digested hOR1D2 PCR product and pET21a expression vector
- Ligation reactions for inserting hOR1D2 into pET21a vector, and transformations of DH5alpha cells with ligation reactions
October 2018
- Completed analysis of USAFA iGEM plasmid intended for DNA part submission
- Cadet McDonough discussed human practices considerations with Col. Barnes, USMA Dept. of English and Philosophy
- Took delivery of synthetic operon plasmid from GeneWiz
- Analysis of pET21A plasmid containing hOR1D2 insert
- Transform BL21(DE3) cells with hOR1D2/pET21a and synthetic operon plasmids
- Transform BL21(DE3) cells with synthetic operon plasmid
- Attend Jamboree