Team:XJTLU-CHINA/Basic Part
Basic Parts
| Part Number | Part Name | Function | Designer | Length |
|---|---|---|---|---|
| BBa_K2619000 | STEAP3 | A metalloreductase can enhance exosome biogenesis through Endoplasmic reticulum/Golgi-independent or non-classical pathway. | Shiyu Liu | 1479bp |
| BBa_K2619001 | SDC4 | Syndecan-4 was used to support budding of endosomal membranes through syndecan-4/syntenin (ESCRT-dependent classical) pathway. | Shiyu Liu | 609bp |
| BBa_K2619002 | NadB | A gene encodes L-aspartate oxidase which possibly boosts cellular metabolism by tuning up the citric acid cycle. We used it to accelerate exosome biogenesis. | Shiyu Liu | 1008bp |
| BBa_K2619003 | CD63 | a transmembrane protein which densely distributes on exosomal membrane. We used the transmembrane domain of CD63 in our design. | Haoping Cheng | 714bp |
| BBa_K2619004 | nluc | A newly developed luciferase which was expressed as an indicator of exosome quantity by combining to CD63. | Shiyu Liu | 510bp |
| BBa_K2619005 | C/D box | C/D box contains the structure of k-loop RNA motif which can be recognized by L7Ae. By engineering it at the 3'-UTR of cargo mRNA, CD63-L7Ae can load that RNA into exosome. | Peng Chai | 72bp |
| BBa_K2619006 | 3 loop | 3 loop are the stem loops cognate with MS2, which can be recognized by MS2. By engineering it at the 3'-UTR of cargo mRNA, CD63-MS2 can load that mRNA into exosome | Shiyu Liu | 89bp |
| BBa_K2619007 | Connexin36 | Connexin 36(gap junction protein, Homo sapiens) is a gap junction protein which can form a transmemberane channel to release cargo mRNA. It is expressed predominantly in inferior olive and hippocampus. | Haohui Fang | 966bp |
| BBa_K2619008 | Connexin43 | Connexin 43 (gap junction protein alpha 1, Homo sapiens) is a gap junction protein designed to increase the efficiency of releasing mRNA into target cell. It is expressed predominantly in glial cells. | Peng Chai | 1146bp |
| BBa_K2619009 | GNSTM | An attachment site which prevents targeting peptide RVG from degradation without effecting the interaction at targeted cells. We utilized it to increase the targeting drug-delivery efficiency of exosomes. | Haoping Cheng | 15bp |
| BBa_K2619010 | VEEV RSS | VEEV RSS is a stabilizing sequence from the 3′UTR of the Venezuelan equine encephalitis virus (VEEV),which can stabilize mRNA for up to 36 hours. | Shiyu Liu | 392bp |
| BBa_K2619011 | HSP70 | A chaperone that facilitates the degradation progress of Lewy body and Lewy neurites, which is a characteristic symptom of Parkinson’s disease. Its mRNA was loaded in exosomes as therapeutic RNA. | Miaoyan Li | 1935bp |
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Xi'an Jiaotong-Liverpool University
111 Ren'ai Road, Suzhou, China
215123
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igem@xjtlu.edu.cn