Team:XJTLU-CHINA/Basic Part

Basic Parts

Part Number Part Name Function Designer Length
BBa_K2619000 STEAP3 A metalloreductase can enhance exosome biogenesis through Endoplasmic reticulum/Golgi-independent or non-classical pathway. Shiyu Liu 1479bp
BBa_K2619001 SDC4 Syndecan-4 was used to support budding of endosomal membranes through syndecan-4/syntenin (ESCRT-dependent classical) pathway. Shiyu Liu 609bp
BBa_K2619002 NadB A gene encodes L-aspartate oxidase which possibly boosts cellular metabolism by tuning up the citric acid cycle. We used it to accelerate exosome biogenesis. Shiyu Liu 1008bp
BBa_K2619003 CD63 a transmembrane protein which densely distributes on exosomal membrane. We used the transmembrane domain of CD63 in our design. Haoping Cheng 714bp
BBa_K2619004 nluc A newly developed luciferase which was expressed as an indicator of exosome quantity by combining to CD63. Shiyu Liu 510bp
BBa_K2619005 C/D box C/D box contains the structure of k-loop RNA motif which can be recognized by L7Ae. By engineering it at the 3'-UTR of cargo mRNA, CD63-L7Ae can load that RNA into exosome. Peng Chai 72bp
BBa_K2619006 3 loop 3 loop are the stem loops cognate with MS2, which can be recognized by MS2. By engineering it at the 3'-UTR of cargo mRNA, CD63-MS2 can load that mRNA into exosome Shiyu Liu 89bp
BBa_K2619007 Connexin36 Connexin 36(gap junction protein, Homo sapiens) is a gap junction protein which can form a transmemberane channel to release cargo mRNA. It is expressed predominantly in inferior olive and hippocampus. Haohui Fang 966bp
BBa_K2619008 Connexin43 Connexin 43 (gap junction protein alpha 1, Homo sapiens) is a gap junction protein designed to increase the efficiency of releasing mRNA into target cell. It is expressed predominantly in glial cells. Peng Chai 1146bp
BBa_K2619009 GNSTM An attachment site which prevents targeting peptide RVG from degradation without effecting the interaction at targeted cells. We utilized it to increase the targeting drug-delivery efficiency of exosomes. Haoping Cheng 15bp
BBa_K2619010 VEEV RSS VEEV RSS is a stabilizing sequence from the 3′UTR of the Venezuelan equine encephalitis virus (VEEV),which can stabilize mRNA for up to 36 hours. Shiyu Liu 392bp
BBa_K2619011 HSP70 A chaperone that facilitates the degradation progress of Lewy body and Lewy neurites, which is a characteristic symptom of Parkinson’s disease. Its mRNA was loaded in exosomes as therapeutic RNA. Miaoyan Li 1935bp

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