The blood brain barrier (BBB) is formed by many components including endothelial cells of the capillary wall, astrocyte and pericytes. It is the largest interface between the blood and the central nervous system and is situated between the blood and the interstitial fluid. Our RVG construct is designed to steer exosome to cross the obstacle
Delivering exosome through BBB is an extremely complex process.
The affinity of RVG to nicotinic acetylcholine receptor (nAchR) is critical to clathrin and caveolae-mediated transcytosis,
which is the main mechanism of guiding exosome through the barrier. To study the ability of our engineered exosomes to pass the BBB,
we simplify the whole process into passing endothelial cells and study the aptitude of RVG by modeling the binding properties to the receptor side of the endothelial cell membrane.
CX43, a family member of gap junction proteins,
can only help us to deliver exosome if it is open and exchange fusion signal with neuron cell. Here,
we present a model demonstrating a macroscopic situation in gap junctions,
and thereby ensuring the functionality of CX43 as a cytosolic delivery helper.