Team:XMU-China/Composite Part
Composite Parts
We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is BBa_K2623030, which links BBa_K2623024 and BBa_K2623025, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher.
| Favorite | Register Name | Code used in Lab | Description | Types | Lenth | Designer |
|---|---|---|---|---|---|---|
| R | BBa_K2623030 | NG9-1 | A composite part linked by BBa_K2623024 and BBa_K2623025. | Composite | 3410 bp | Yang Liang |
| BBa_K2623011 | Kai 2 | Phosphorylation pathsway builder of Kai | Composite | 4444 bp | Gezhi Xiao | |
| BBa_K2623013 | Kai 1 3 | Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC | Composite | 3978 bp | Hongling Liu | |
| BBa_K2623014 | CAHS 1 | Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator) | Composite | 2891 bp | Hongling Liu | |
| BBa_K2623015 | SAHS 1 | Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator) | Composite | 1425 bp | Jun Dai | |
| BBa_K2623016 | SAHS 2 | Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator) | Composite | 1431 bp | Yuanjun Shang | |
| BBa_K2623021 | NG1 | BBa_K2623022 with GFP at its N-termini | Reporter | 1657 bp | Niangui Cai | |
| BBa_K2623022 | NG2 | Bacterial outer membrane protein A (OmpA) fused with SpyTag at its C-termini | Composite | 910 bp | Qiupeng Wang | |
| BBa_K2623023 | NG3 | Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini | Reporter | 1645 bp | Junhong Chen | |
| BBa_K2623024 | NG4 | A non-GFP form of the BBa_K2623023 | Composite | 898 bp | Jiyang Zheng | |
| BBa_K2623025 | NG5 | Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1. | Reporter | 2613 bp | Ruofan Yang | |
| BBa_K2623026 | NG6 | Archaeal ribosomal protein protein L7Ae fused with SpyCatcher. | Composite | 2317 bp | Yunyun Hu | |
| BBa_K2623028 | NG8-1 | A composite part linked by BBa_K2623022 and BBa_K2623025 | Composite | 3410 bp | Niangui Cai | |
| BBa_K2623029 | NG8-2 | A composite part linked by BBa_K2623022 and BBa_K2623026. | Composite | 2698 bp | Ye Qiang | |
| BBa_K2623031 | NG9-2 | A composite part linked by BBa_K2623024 and BBa_K2623026 | Composite | 2698 bp | Junhong Chen | |
| BBa_K2623032 | NG10 | A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027 | Composite | 4153 bp | Niangui Cai |
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