Mar
- Our team was formally founded.
- All student members learned the fundamental knowledge of molecular cloning and iGEM.
- All student members looked through the previous excellent projects and learned the basic notion of synthetic biology and iGEM.
Apr
- We were divided into four groups and started to brainstorm separately (and we reported each’s progress every weekend).
- Our team was formally accepted by iGEM.
May
- Our formal project was confirmed.
- The pre-experiment plan was confirmed.
- The division of labor was confirmed.
Jun Wk1-3
-All student members learned laboratory fundamental qualities and received molecular cloning operation training.
Jun Wk 4
- 2018 DNA Distribution Kit Plates were received.
- We transformed the biobricks which we needed.
- BBa_J04450 constructed to get the backbone.
Jul Wk 3
– The genetic circuit of the TDP began to be built.
Jul Wk 4
– Interlab experiments.
– Verified the activity of EpCAM.
Aug Wk 1
– The genetic circuit of the Kai began to be built.
Sep Wk 2
- GFP on BBa_K2623021 and BBa_K2623023 successfully expressed.
- BBa_K2623004 constructed.
- BBa_K2623005 constructed.
- BBa_K2623006 constructed.
- First time successful purification of high-purity SAHS protein.
- Coating experiment on microfluidic chips succeeded.
- Started bacterial freeze-drying experiment.
Sep Wk 3
- BBa_E1010 constructed to get the mRFP reporter.
- BBa_K2623011 constructed.
- BBa_K2623007 constructed.
- Competition experiment succeeded.
- Started protein freeze-drying experiment.
– Verified the feasibility of “competition method” successfully.
Oct Wk 1
- BBa_K2623027 constructed.
- BBa_K2623013 constructed.
- NG2+5, NG2+6, NG4+5, NG4+6 constructed.
– Verified that As Cas12a could be activated.
Oct Wk 2
-
BBa_K2623028,
BBa_K2623029,
BBa_K2623030,
BBa_K2623031 successfully constructed and the fluorescence intensity of OMVs isolated from E.coli containing these four parts were detected by flow cytometer
– Verified the successful combination between SYL3C aptamer and EpCAM.
– Transferred molecular signal to fluorescence signal with DNase Alert.
Oct Wk 3
– Verified the transfer from protein signal to the fluorescent one successfully
