Team:XMU-China/Parts
This year we have designed more than 20 parts, half are basic parts, the other are composite ones. The parts are all designed by ourselves and most of them have been tested to insure their reliability. To see their specific function, you can click here to learn about our project, or browse the specific page on parts.igem.org by clicking on the part numbers.
This year we have designed eleven basic parts mostly from TDP protein systems and Kai ABC systems. BBa_K2623008 is our favorite basic part, which is the core part of the TDP circuit. This part contains the coding region of the Hypsibius dujardini (Water bear) (Macrobiotus dujardini) CAHS gene. Cytosolic abundant heat soluble proteins acts as a molecular shield in water-deficient condition. Tardigrade-specific intrinsically disordered proteins (TDPs) are essential for desiccation tolerance by forming non-crystalline amorphous solids upon desiccation, and this vitrified state mirrors their protective capabilities.
| Favorite | Register Name | Code used in Lab | Description | Types | Lenth | Designer |
|---|---|---|---|---|---|---|
| R | BBa_K2623008 | CAHS | CAHS coding region | Coding | 714 bp | Weizhao Li |
| BBa_K2623001 | KaiA | KaiA coding region | Coding | 852 bp | Yi Lin | |
| BBa_K2623002 | KaiB | KaiB coding region | Coding | 309 bp | Yuhan Qin | |
| BBa_K2623003 | KaiC | KaiC coding region | Coding | 1560 bp | Xinyi Ren | |
| BBa_K2623004 | SasA | SasA coding region | Coding | 1167 bp | Yi Lin | |
| BBa_K2623005 | CikA | CikA coding region | Coding | 2265 bp | Yuhan Qin | |
| BBa_K2623006 | RpaA | RpaA coding region | Coding | 753 bp | Xinyi Ren | |
| BBa_K2623007 | SAHS | SAHS coding region | Coding | 510 bp | Yingming Zhu | |
| BBa_K2623009 | pKaiBC | pKaiBC promoter | Regulatory | 89 bp | Gezhi Xiao | |
| BBa_K2623040 | OmpA leader | OmpA leader sequence | Coding | 72 bp | Niangui Cai | |
| BBa_K2623027 | NG7 | siRNA with C/Dbox RNA structure to bind with L7Ae (BBa_K2623026) | Composite | 1447 bp | Yunyun Hu |
We have designed a series of composite parts this year, and most of them could be useful tools for constructing other OMVs and KaiABC systems. And our favorite composite part is BBa_K2623030, which links BBa_K2623024 and BBa_K2623025, coding SpyTag-OmpA and SpyCatcher-L7Ae-RFP to be encapsulated into OMVs through the isopeptide bond formed between SpyTag/SpyCatcher.
| Favorite | Register Name | Code used in Lab | Description | Types | Lenth | Designer |
|---|---|---|---|---|---|---|
| R | BBa_K2623030 | NG9-1 | A composite part linked by BBa_K2623024 and BBa_K2623025. | Composite | 3410 bp | Yang Liang |
| BBa_K2623011 | Kai 2 | Phosphorylation pathsway builder of Kai | Composite | 4444 bp | Gezhi Xiao | |
| BBa_K2623013 | Kai 1 3 | Kai's phosphorylation core oscllator builder and SfYFP reportor for pKaiBC | Composite | 3978 bp | Hongling Liu | |
| BBa_K2623014 | CAHS 1 | Cytosolic-abundant heat soluble protein "CAHS " (promoter, RBS, RFP, lacI and double terminator) | Composite | 2891 bp | Hongling Liu | |
| BBa_K2623015 | SAHS 1 | Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, RFP and double terminator) | Composite | 1425 bp | Jun Dai | |
| BBa_K2623016 | SAHS 2 | Secretory-abundant heat soluble protein "SAHS " (promoter, RBS, amilCP and double terminator) | Composite | 1431 bp | Yuanjun Shang | |
| BBa_K2623021 | NG1 | BBa_K2623022 with GFP at its N-termini | Reporter | 1657 bp | Niangui Cai | |
| BBa_K2623022 | NG2 | Bacterial outer membrane protein A (OmpA) fused with SpyTag at its C-termini | Composite | 910 bp | Qiupeng Wang | |
| BBa_K2623023 | NG3 | Bacterial outer membrane protein A (OmpA) fused with SpyTag and GFP at its N-termini | Reporter | 1645 bp | Junhong Chen | |
| BBa_K2623024 | NG4 | A non-GFP form of the BBa_K2623023 | Composite | 898 bp | Jiyang Zheng | |
| BBa_K2623025 | NG5 | Archaeal ribosomal protein protein L7Ae fused with SpyCatcher and mRFP1. | Reporter | 2613 bp | Ruofan Yang | |
| BBa_K2623026 | NG6 | Archaeal ribosomal protein protein L7Ae fused with SpyCatcher. | Composite | 2317 bp | Yunyun Hu | |
| BBa_K2623028 | NG8-1 | A composite part linked by BBa_K2623022 and BBa_K2623025 | Composite | 3410 bp | Niangui Cai | |
| BBa_K2623029 | NG8-2 | A composite part linked by BBa_K2623022 and BBa_K2623026. | Composite | 2698 bp | Ye Qiang | |
| BBa_K2623031 | NG9-2 | A composite part linked by BBa_K2623024 and BBa_K2623026 | Composite | 2698 bp | Junhong Chen | |
| BBa_K2623032 | NG10 | A composite part linked by BBa_K2623024, BBa_K2623026 and BBa_K2623027 | Composite | 4153 bp | Niangui Cai |
