Difference between revisions of "Team:ZJUT-China/InterLab"
| Line 1: | Line 1: | ||
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| + | display:block; | ||
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| + | font-family: "Arial Black", Gadget, sans-serif; | ||
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| + | padding: 5px 0px; | ||
| + | text-align: left; | ||
| + | color: #484848; | ||
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| + | |||
| + | /* Links */ | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper a { | ||
| + | color: #5bc7d8; | ||
| + | font-weight: bold; | ||
| + | text-decoration: underline; | ||
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| + | transition: all 0.4s ease; | ||
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| + | -ms-transition: all 0.4s ease; | ||
| + | -o-transition: all 0.4s ease; | ||
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| + | color: #f8b732; | ||
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| + | border: 1px solid #928b8b; | ||
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| + | font-size: 130%; | ||
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| + | font-size: 105%; | ||
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| + | /* table headers */ | ||
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| + | background-color:#c4baba; | ||
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| + | border-collapse: collapse; | ||
| + | font-size: 110%; | ||
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| + | vertical-align: text-top; | ||
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| + | font-size: 130%; | ||
| + | font-family: Arial, Helvetica, sans-serif; | ||
| + | padding:0px 20px; | ||
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| + | |||
| + | |||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper ul ul li, .igem_2018_team_content .igem_2018_team_column_wrapper ul ul ul li, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper ul ol li, .igem_2018_team_content .igem_2018_team_column_wrapper ul ul ol li, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper ol ol li, .igem_2018_team_content .igem_2018_team_column_wrapper ul ol ul li, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper ol ul li, .igem_2018_team_content .igem_2018_team_column_wrapper ul ol ol li, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper ol ul ul li, .igem_2018_team_content .igem_2018_team_column_wrapper ol ol ul li, | ||
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| + | .igem_2018_team_content .igem_2018_team_column_wrapper .column.full_size { width:96%; } | ||
| + | |||
| + | /* 66% */ | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .column.two_thirds_size { width: 62.6%; } | ||
| + | |||
| + | /* 33% */ | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .column.third_size { width: 29.3%; } | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | /*styling for all images*/ | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .column.full_size img, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .column.two_thirds_size img, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .column.third_size img { | ||
| + | margin-bottom: 15px; | ||
| + | max-width: 100%; | ||
| + | } | ||
| + | |||
| + | |||
| + | /* page break */ | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .clear { | ||
| + | clear:both; | ||
| + | } | ||
| + | /*add extra space to page break with clear class*/ | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .clear.extra_space { | ||
| + | height: 30px; | ||
| + | } | ||
| + | |||
| + | /* horizontal line to divide the page*/ | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .line_divider { | ||
| + | border-top: 1px solid #c4baba; | ||
| + | margin: auto; | ||
| + | width: 98%; | ||
| + | } | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | /*support classes*/ | ||
| + | /**************************************************************************************************************************************************************************************************/ | ||
| + | |||
| + | |||
| + | /*Button */ | ||
| + | /************************************************/ | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .button_link { | ||
| + | font-size: 130%; | ||
| + | margin: 30px auto; | ||
| + | text-align: center; | ||
| + | } | ||
| + | |||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .button_link a { | ||
| + | background-color: #5bc7d8; | ||
| + | color: #635d5d !important; | ||
| + | font-weight: bold; | ||
| + | margin: auto; | ||
| + | text-decoration: none !important; | ||
| + | padding: 10px 15px; | ||
| + | } | ||
| + | |||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .button_link a:hover { | ||
| + | background-color: #f8b732 !important; | ||
| + | } | ||
| + | |||
| + | |||
| + | |||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight { | ||
| + | padding: 15px 20px; | ||
| + | } | ||
| + | |||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight p, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight h1, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight h2, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight h3, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight h4, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight h5, | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight h6 { | ||
| + | padding: 5px 15px; | ||
| + | } | ||
| + | |||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight.decoration_background { | ||
| + | background-color: #e4dede; | ||
| + | } | ||
| + | |||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight.decoration_A_top { | ||
| + | border-top: 4px solid #5bc7d8; | ||
| + | } | ||
| + | |||
| + | |||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight.decoration_A_full { | ||
| + | border: 4px solid #5bc7d8; | ||
| + | } | ||
| + | |||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight.decoration_B_top { | ||
| + | border-top: 4px solid #f8b732 | ||
| + | } | ||
| + | |||
| + | |||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .highlight.decoration_B_full { | ||
| + | border: 4px solid #f8b732; | ||
| + | } | ||
| + | |||
| + | |||
| + | |||
| + | |||
| + | /*mobile*/ | ||
| + | /**************************************************************************************************************************************************************************************************/ | ||
| + | |||
| + | |||
| + | /* 1800px */ | ||
| + | /************************************************/ | ||
| + | @media only screen and (max-width: 1800px) { | ||
| + | .igem_2018_team_content { width: 100%;} | ||
| + | .igem_2018_team_menu {display:block;} | ||
| + | } | ||
| + | |||
| + | /* 1400px */ | ||
| + | /************************************************/ | ||
| + | @media only screen and (max-width: 1400px) { | ||
| + | .igem_2018_team_menu .menu_item { font-size:100%;} | ||
| + | .igem_2018_team_menu .submenu .submenu_item { font-size:90%;} | ||
| + | .igem_2018_team_menu {display:block;} | ||
| + | } | ||
| + | |||
| + | @media only screen and (max-width: 1001px) { | ||
| + | .igem_2018_team_menu {display:block;} | ||
| + | } | ||
| + | |||
| + | /* 1000px */ | ||
| + | /************************************************/ | ||
| + | @media only screen and (max-width: 1000px) { | ||
| + | .igem_2018_team_content {width:100%; margin-left:0px;} | ||
| + | .igem_2018_team_menu {display:none; margin-top: 45px; min-width:50%; width:50%;} | ||
| + | .igem_2018_team_mobile_bar {display:block;} | ||
| + | .igem_2018_team_content .igem_2018_team_column_wrapper .column.full_size, .igem_2018_team_content .igem_2018_team_column_wrapper .column.two_thirds_size,.igem_2018_team_content .igem_2018_team_column_wrapper .column.third_size {width:96%; } | ||
| + | |||
| + | } | ||
| + | |||
| + | |||
| + | @media only screen and (max-width: 500px) { | ||
| + | .igem_2018_team_menu {min-width:100%; width:100%; } | ||
| + | } | ||
| + | |||
| + | |||
| + | /**************************************************************************************************************************************************************************************************/ | ||
| + | |||
| + | /*my styles */ | ||
| + | /************************************************/ | ||
| + | img{ | ||
| + | max-width：100%; | ||
| + | |||
| + | } | ||
| + | .homepage_img{ | ||
text-align: center; | text-align: center; | ||
| + | background: url(5.jpg); | ||
| + | height: 800px; | ||
| + | width: 1500px; | ||
| + | background-size:cover; | ||
} | } | ||
| − | . | + | |
| + | .container{ | ||
| + | padding:0 30px 0 30px; | ||
| + | display: block; | ||
| + | } | ||
| + | .homepage_img{ | ||
| + | text-align: center; | ||
| + | padding:0 0 20px 0; | ||
| + | } | ||
| + | /* 顶栏固定 */ | ||
| + | .topbar{ | ||
| + | position: fixed; | ||
| + | top: 0px; | ||
| + | height: 51px; | ||
| + | width: 100%; | ||
| + | padding-top: 18px; | ||
| + | } | ||
| + | /* 下拉按钮样式 */ | ||
| + | .dropbkgd{ | ||
| + | height: 51px; | ||
| + | background-image: url(https://static.igem.org/mediawiki/2018/7/76/T--ZJUT-China--topbar.gif); | ||
| + | clear: both; | ||
| + | width: 100%; | ||
| + | } | ||
| + | .drop{ | ||
| + | color: transparent; | ||
| + | font-size: 20px; | ||
| + | line-height: 51px; | ||
| + | width: 100%; | ||
| + | height: 100%; | ||
| + | } | ||
| + | |||
| + | .dropposition{ | ||
| + | position: absolute; | ||
| + | left: 240px; | ||
| + | width: 100%; | ||
| + | height: 51px; | ||
| + | } | ||
| + | .drop:hover{ | ||
| + | background-color: #BACD32; | ||
| + | color: white; | ||
| + | } | ||
| + | .drop a{ | ||
| + | color: transparent; | ||
| + | text-decoration: none; | ||
| + | display: inline-block; | ||
| + | width: 100%; | ||
| + | height: 100%; | ||
| + | } | ||
| + | .drop a:hover{ | ||
| + | color: white; | ||
| + | } | ||
| + | |||
| + | /* 容器 <div> - 需要定位下拉内容 */ | ||
| + | .dropdown1, | ||
| + | .dropdown2, | ||
| + | .dropdown3, | ||
| + | .dropdown4, | ||
| + | .dropdown5, | ||
| + | .dropdown6, | ||
| + | .dropdown7, | ||
| + | .dropdown8{ | ||
position: relative; | position: relative; | ||
| + | display: inline-block; | ||
| + | float:left; | ||
| + | text-align: center; | ||
| + | width: 8%; | ||
| + | height: 100%; | ||
| + | } | ||
| + | .dropdown1 { | ||
| + | width: 7%; | ||
| + | } | ||
| + | .dropdown2{ | ||
| + | width: 7%; | ||
| + | left: 25px; | ||
| + | } | ||
| + | .dropdown3{ | ||
| + | width: 8%; | ||
| + | left: 40px; | ||
| + | } | ||
| + | .dropdown4{ | ||
| + | width:7%; | ||
| + | left:60px; | ||
| + | } | ||
| + | .dropdown5{ | ||
| + | width: 7%; | ||
| + | left:70px; | ||
| + | } | ||
| + | .dropdown6{ | ||
| + | width:15.2%; | ||
| + | left: 87px; | ||
| + | } | ||
| + | .dropdown7{ | ||
| + | width:12%; | ||
| + | left: 90px; | ||
| + | } | ||
| + | .dropdown8{ | ||
| + | width:12%; | ||
| + | left: 100px; | ||
| + | } | ||
| + | /* 下拉内容 (默认隐藏) */ | ||
| + | .dropdown-content { | ||
| + | display: none; | ||
| + | position: absolute; | ||
| + | color: #DACD32; | ||
| + | width: 180px; | ||
| + | box-shadow: 0px 8px 16px 0px rgba(0,0,0,0.2); | ||
| + | float: left; | ||
| + | background-color: rgba(0,0,0,0.2); | ||
| + | } | ||
| + | .dropdown6 .dropdown-content{ | ||
| + | width:230.4px; | ||
| + | } | ||
| + | |||
| + | /* 下拉菜单的链接 */ | ||
| + | .dropdown-content a { | ||
| + | color: white; | ||
| + | text-decoration: none; | ||
| + | display: block; | ||
| + | width: 100%; | ||
| + | height: 100%; | ||
| + | } | ||
| + | |||
| + | /* 鼠标移上去后修改下拉菜单链接颜色 */ | ||
| + | .dropdown-content a:hover {background-color: #7D7DFF} | ||
| + | |||
| + | /* 在鼠标移上去后显示下拉菜单 */ | ||
| + | .dropdown2:hover .dropdown-content { | ||
| + | display: block; | ||
| + | } | ||
| + | .dropdown3:hover .dropdown-content { | ||
| + | display: block; | ||
| + | } | ||
| + | .dropdown4:hover .dropdown-content { | ||
| + | display: block; | ||
| + | } | ||
| + | .dropdown5:hover .dropdown-content { | ||
| + | display: block; | ||
| + | } | ||
| + | .dropdown6:hover .dropdown-content { | ||
| + | display: block; | ||
| + | } | ||
| + | .dropdown7:hover .dropdown-content { | ||
| + | display: block; | ||
| + | } | ||
| + | .dropdown8:hover .dropdown-content { | ||
| + | display: block; | ||
| + | } | ||
| + | |||
| + | .container .col-10 { | ||
| + | margin:auto; | ||
| + | max-width: 1400px; | ||
| + | width:88%; | ||
| + | padding: 20px 0 0 0 ; | ||
| + | } | ||
| + | |||
| + | .container .col-10 h1 { font-size: 210%;} | ||
| + | .container .col-10 h2 { font-size: 190%;} | ||
| + | .container .col-10 h3 { font-size: 170%;} | ||
| + | .container .col-10 h4 { font-size: 150%;} | ||
| + | .container .col-10 h5 { font-size: 140%;} | ||
| + | .container .col-10 h6 { font-size: 130%;} | ||
| + | |||
| + | |||
| + | /* styling for the titles h1, h2*/ | ||
| + | .container .col-10 h1, .container .col-10 h2 { | ||
| + | border-bottom:0px; | ||
| + | font-family: 'Tempus Sans ITC',georgia !important; | ||
| + | padding: 10px 0px; | ||
| + | } | ||
| + | .container .col-10 h1{ | ||
| + | font-size: 38px!important; | ||
| + | padding: 20 20 20 20!important; | ||
| + | text-align: center; | ||
| + | color: #9ACD32; | ||
| + | } | ||
| + | .container .col-10 h2 { | ||
| + | font-size: 30px!important; | ||
| + | color: #6495ED; | ||
| + | } | ||
| + | |||
| + | |||
| + | |||
| + | /* styling for the titles h3, h3, h5, h6 */ | ||
| + | .container .col-10 h3, | ||
| + | .container .col-10 h4, | ||
| + | .container .col-10 h5, | ||
| + | .container .col-10 h6 { | ||
| + | border-bottom:0px; | ||
| + | color: #9ACD32; | ||
| + | font-family: "Arial Black", Gadget, sans-serif; | ||
| + | padding: 5px 0px; | ||
| + | } | ||
| + | |||
| + | /* text */ | ||
| + | .container .col-10 p { | ||
| + | font-family: Arial, Helvetica, sans-serif; | ||
| + | padding: 5px 0px; | ||
| + | text-align: left; | ||
| + | color: #000000; | ||
| + | font-size: 20px!important; | ||
| + | } | ||
| + | .img-10{ | ||
| + | text-align: center ; | ||
| + | weight: 600px; | ||
| + | } | ||
| + | .side-bar .igem_2018_title_menu{ | ||
| + | position: fixed; | ||
| + | } | ||
| + | .note{ | ||
| + | font-size:15px; | ||
| + | color:#708090; | ||
| + | text-align: center; | ||
| + | } | ||
| + | .homeindex{ | ||
| + | width: 20%; | ||
min-height: 1px; | min-height: 1px; | ||
| − | |||
float: left; | float: left; | ||
| + | margin: 15px; | ||
text-align: center; | text-align: center; | ||
| + | padding-left: 25px; | ||
| + | |||
} | } | ||
| − | . | + | .homeindex img{ |
| − | + | border-radius: 30px; | |
} | } | ||
| − | . | + | /* 底部 */ |
| − | + | .footer{ | |
| + | background: #eee; | ||
| + | margin-top: 30px; | ||
| + | padding: 30px 0; | ||
| + | width: 100%; | ||
| + | clear: both; | ||
} | } | ||
| − | . | + | .foottop{ |
| − | + | height: 230px; | |
| + | width: 100%; | ||
} | } | ||
| + | .footbottom{ | ||
| + | height:50px; | ||
| + | width: 100%; | ||
| + | } | ||
| + | .footbottom .note{ | ||
| + | padding-top: 40px; | ||
| + | } | ||
| + | .footmessage-left, | ||
| + | .footmessage-right, | ||
| + | .footmessage-middle { | ||
| + | width: 30%; | ||
| + | float: left; | ||
| + | height: 100%; | ||
| + | } | ||
| + | .footmessage-right, img{ | ||
| + | max-width: 100%; | ||
| + | max-height: 100%; | ||
| + | padding-top: 10px; | ||
| + | padding-left: 30px; | ||
| + | } | ||
| + | .footmessage-left img{ | ||
| + | padding-top: 22px; | ||
| + | padding-left: 30px; | ||
| + | max-width: 100%; | ||
| + | max-height: 100%; | ||
| + | } | ||
| + | .footmessage-right h3{ | ||
| + | font-size: 30px; | ||
| + | } | ||
| + | .footmessage-right p{ | ||
| + | font-size: 15px!important; | ||
| + | } | ||
| + | .container .igem_2018_team_content .igem_2018_team_column_wrapper li{ | ||
| + | padding-top: 10px; | ||
| + | } | ||
| + | .container .igem_2018_team_content .igem_2018_team_column_wrapper a{ | ||
| + | font-size: 20px; | ||
| + | } | ||
| + | .container .col-10 .igem_2018_team_content .igem_2018_team_column_wrapper h3{ | ||
| + | text-align: center; | ||
| + | } | ||
| + | .col-2{ | ||
| + | width: 45%; | ||
| + | float: left; | ||
| + | padding-left: 10px; | ||
| + | display: inline-block; | ||
| + | } | ||
| + | |||
</style> | </style> | ||
| − | <!-- | + | |
| − | < | + | |
| − | + | ||
| − | + | ||
| − | < | + | <!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> |
| − | < | + | <!--- THIS IS WHERE THE HTML BEGINS ---> |
| − | + | <!-------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------> | |
| − | + | ||
| − | + | <head> | |
| − | + | ||
| − | + | <!-- This tells the browser that your page is responsive --> | |
| − | + | <meta name="viewport" content="width=device-width, initial-scale=1"> | |
| − | + | ||
| − | + | </head> | |
| − | + | <!-- 导航栏 --> | |
| − | + | <div class="topbar"> | |
| − | + | <div class="dropbkgd"> | |
| − | + | <div class="dropposition"> | |
| − | + | <div class="dropdown1"> | |
| − | + | <div class="drop"> | |
| − | + | <a href="https://2018.igem.org/Team:ZJUT-China">Home</a> | |
| − | + | </div> | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | < | + | |
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
| − | + | ||
</div> | </div> | ||
| − | <div class=" | + | <div class="dropdown2"> |
| − | < | + | <div class="drop"> |
| − | < | + | Team |
| + | <div class="dropdown-content"> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Team">Team Members</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Attributions">Attributions</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Collaborations">Collaborations</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="dropdown3"> | ||
| + | <div class="drop"> | ||
| + | Project | ||
| + | <div class="dropdown-content"> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Description" >Description</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Design">Design</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Results">Results</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="dropdown4"> | ||
| + | <div class="drop"> | ||
| + | Parts | ||
| + | <div class="dropdown-content"> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Parts">Overview</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Basic_Part">Basic parts</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Composite_Part">Composite parts</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Part_Collection" >Part collection</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="dropdown5"> | ||
| + | <div class="drop"> | ||
| + | Lab | ||
| + | <div class="dropdown-content"> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Notebook">Notebook</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Protocols2018">Protocols</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Safety">Safety</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/InterLab" >Interlab</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="dropdown6"> | ||
| + | <div class="drop"> | ||
| + | Human Practices | ||
| + | <div class="dropdown-content"> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Human_Practices">Integrated hp</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/PublicEngagement2018">Public Engagement</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Public_Engagement8">Education</a> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Collaboration2018">Collaboration</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="dropdown7"> | ||
| + | <div class="drop"> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Model"> Modelling</a> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="dropdown8"> | ||
| + | <div class="drop"> | ||
| + | <a href="https://2018.igem.org/Team:ZJUT-China/Achievement2018"> Achievement</a> | ||
| + | </div> | ||
</div> | </div> | ||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
| − | |||
</div> | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
| − | <div class="column judges-will-not-evaluate middle" > | + | <!-- 首页大图 --> |
| − | + | <div class="homepage_img"> | |
| − | + | </div> | |
| − | + | <!-- 主体 --> | |
| − | + | <div class="container"> | |
| − | + | <div class="col-10"> | |
| − | + | <div class="igem_2018_team_content"> | |
| − | + | <div class="igem_2018_team_column_wrapper"> | |
| − | + | <div class="clear extra_space"></div> | |
| − | + | <div class="clear extra_space"></div> | |
| − | + | <!--interlab--> | |
| − | + | <div class="column full_size judges-will-not-evaluate "> | |
| − | + | <h1>Interlab</h1> | |
| − | + | <p>Our team participated in the Fifth International InterLaboratory Measurement Study this year. The interlab study aims to solve the problem of reliability and repeatability in synthetic biology study. This year we were asked to measure green fluorescent protein, which is used as a measurement marker. We followed the experiment protocol strictly to make sure our data is valid. After two weeks lab work, we got the data we need after several attempts.</p> | |
| − | + | </div> | |
| − | + | <div class="column judges-will-not-evaluate middle" > | |
| − | + | <h1>Calibration Protocol</h1> | |
| − | + | <p>Three calibration measurement were done by the calibration protocol, including an OD reference point at 600nm, one particle standard curve and one fluorescent standard curve.</p> | |
| − | + | <div class="note" style="color:red">(Plate Reader: Molecular Device, Spectramax M5)</div> | |
| − | + | <br><br> | |
| − | + | <h3>★ OD600 reference point ★ </h3> | |
| − | + | <br> | |
| − | + | <table border="1"> | |
| − | + | <thead> | |
| − | + | <tr> | |
| − | + | <th></th> | |
| − | + | <th>LUDOX CL-X</th> | |
| − | + | <th>H2O</th> | |
| − | + | </tr> | |
| − | + | </thead> | |
| − | + | <tbody> | |
| − | + | <tr> | |
| − | + | <th>Replicate 1</th> | |
| − | + | <th class="bluetable">0.066</th> | |
| − | + | <th class="bluetable">0.0285</th> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <th>Replicate 2</th> | |
| − | + | <th>0.059</th> | |
| − | + | <th>0.029</th> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <th>Replicate 3</th> | |
| − | + | <th>0.068</th> | |
| − | + | <th>0.026</th> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <th>Replicate 4</th> | |
| − | + | <th>0.064</th> | |
| − | + | <th>0.028</th> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <th>Arith. Mean</th> | |
| − | + | <th>0.064</th> | |
| − | + | <th>0.028</th> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <th>Corrected Abs600</th> | |
| − | + | <th> 0.036</th> | |
| − | + | <th></th> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <th>Reference OD600</th> | |
| − | + | <th>OD600</th> | |
| − | + | <th></th> | |
| − | + | </tr> | |
| − | + | <tr> | |
| − | + | <th>OD600/Abs600</th> | |
| − | + | <th>1.728</th> | |
| − | + | <th></th> | |
| − | + | </tr> | |
| − | + | </tbody> | |
| − | + | </table> | |
| − | + | <div class="note" style="color:red">Form1.OD reference point</div> | |
| − | + | <br><br> | |
| − | + | <div class="col-2"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2018/4/42/T--ZJUT-China--Particle_Standard_Curve.png" alt="Particle_Standard_Curve.png"> | |
| − | + | <div class="note" style="color:red">Fig1.Particle standard curve</div> | |
| − | + | </div> | |
| − | + | <div class="col-2"> | |
| − | + | <img src="https://static.igem.org/mediawiki/2018/f/fc/T--ZJUT-China--Particle_Standard_Curve_%28log_scale%29e.png" height="259.25px" alt="Particle_Standard_Curve_(log_scale)e"> | |
| − | + | <div class="note" style="color:red">Fig2.Particle standard curve (log_scale)</div> | |
| − | + | </div> | |
| − | + | <div class="col-2"> | |
| + | <img src="https://static.igem.org/mediawiki/2018/c/c5/T--ZJUT-China--Fluorescein_Standard_Curve.png" alt="Fluorescein_Standard_Curve"> | ||
| + | <div class="note" style="color:red">Fig3.Fluorescein standard curve</div> | ||
| + | </div> | ||
| + | <div class="col-2"> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/c/ca/T--ZJUT-China--Fluorescein_Standard_Curve_%28log_scale%29.png" alt="Fluorescein_Standard_Curve_%28log_scale%29"> | ||
| + | <div class="note" style="color:red">Fig4.Fluorescein standard curve (log_scale)</div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <div class="column judges-will-not-evaluate middle" > | ||
| + | <h1>Cell measurement</h1> | ||
| + | <h2>Transformation</h2> | ||
| + | <p>Transform Escherichia coli DH5α with these following plasmids:</p> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <th>Device</th> | ||
| + | <th>Part Number</th> | ||
| + | <th>Location</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Positive control</th> | ||
| + | <th>BBa_R0040</th> | ||
| + | <th>Well 2D</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Negative control</th> | ||
| + | <th>Ba_I20270</th> | ||
| + | <th>Well 2B</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Test Device 1</th> | ||
| + | <th>BBa_J364000</th> | ||
| + | <th>Well 2F</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Test Device 2</th> | ||
| + | <th>BBa_J364001</th> | ||
| + | <th>Well 2H</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Test Device 3</th> | ||
| + | <th>BBa_J364002</th> | ||
| + | <th>Well 2J</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Test Device 4</th> | ||
| + | <th>BBa_J364007</th> | ||
| + | <th>Well 2L</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Test Device 5</th> | ||
| + | <th>BBa_J364008</th> | ||
| + | <th>Well 2N</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Test Device 6</th> | ||
| + | <th>BBa_J364009</th> | ||
| + | <th>Well 2P</th> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <div class="note" style="color:red">Form2.Devices (from Distribution Kit, all in pSB1C3 backbone) </div> | ||
| + | <p>The transformation used E.coli DH5α competent cells bought from Tsingke Biological Technology company, and follow the steps in http://parts.igem.org/Help:2017_DNA_Distribution to use the DNA in the Distribution Kit.</p> | ||
| + | <h2>Measurement </h2> | ||
| + | <br> | ||
| + | <h3>For this part of inter lab study, we followed the following protocols: | ||
| + | <br></h3> | ||
| + | <p>1.Grown 8 devices in incubator for 12 hrs at 37 ℃ | ||
| + | <br> | ||
| + | 2.Pick 2 colonies from each of plate and inocubate them on 10mL LB medium with Chloramphenicol. Grow the cells for 16-20hrs at 37°C and 180rpm. | ||
| + | <br> | ||
| + | 3.Measure OD600nm of the overnight cultures and record the data, then dilute to target OD600nm = 0.02 in the falcon tubes. | ||
| + | <br> | ||
| + | 4.Measure the OD600nm and Fl under the same condition as standard curve measurement and use the same 96 wells plates.</p> | ||
| + | <br> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/5/5b/T--ZJUT-China--plate_lay_out.png" alt="plate_lay_out"> | ||
| + | <br><div class="note" style="color:red">Fig5. plate lay out </div> | ||
| + | <br><br> | ||
| + | <div class="column judges-will-not-evaluate middle" > | ||
| + | <h3>★ Fluorescence Raw Readings：★</h3> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/d/d2/T--ZJUT-China--Fluorescence_Raw_Readings.png" alt="Fluorescence Raw Readings"> | ||
| + | <br><br> | ||
| + | <h3>★ Abs600 Raw Readings：★ </h3> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/c/ce/T--ZJUT-China--Abs600_Raw_Readings.png" alt="Abs600 Raw Readings"> | ||
| + | <br><br> | ||
| + | <h3>★ uM Fluorescein / OD ★ </h3> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/b/b6/T--ZJUT-China--11.png" alt="uM Fluorescein / OD"> | ||
| + | <br><br> | ||
| + | <h3>★ Net Fluorescein a.u. ★ </h3> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/5/5a/T--ZJUT-China--Net_Fluoresceina.u.png" alt="Net_Fluoresceina.u."> | ||
| + | <br><br> | ||
| + | <h3>★ Net Abs 600 ★ </h3> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/5/55/T--ZJUT-China--Net_Abs_600.png" alt="Net_Abs_600"> | ||
| + | <br><div class="note" style="color:red">Form3-6. Result of Raw Plate Reader Measurements </div> | ||
| + | </div> | ||
| + | </div> | ||
| + | <!--yincang<table border="1"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Hour 0:</th> | ||
| + | <th>Neg. Control</th> | ||
| + | <th>Pos. Control</th> | ||
| + | <th>Device 1</th> | ||
| + | <th>Device 2</th> | ||
| + | <th>Device 3</th> | ||
| + | <th>Device 4</th> | ||
| + | <th>Device 5</th> | ||
| + | <th>Device 6</th> | ||
| + | <th>LB + Chlor (blank)</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 1</th> | ||
| + | <th>98.891</th> | ||
| + | <th>514.509</th> | ||
| + | <th>170.45</th> | ||
| + | <th>453.211</th> | ||
| + | <th>107.527</th> | ||
| + | <th>377.998</th> | ||
| + | <th>110.335</th> | ||
| + | <th>121.276</th> | ||
| + | <th>100.56</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 2</th> | ||
| + | <th>87.951</th> | ||
| + | <th>520.569</th> | ||
| + | <th>155.638</th> | ||
| + | <th>443.722</th> | ||
| + | <th>103.159</th> | ||
| + | <th>353.38</th> | ||
| + | <th>99.569</th> | ||
| + | <th>117.851</th> | ||
| + | <th>97.983</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 3</th> | ||
| + | <th>89.665</th> | ||
| + | <th>500.61</th> | ||
| + | <th>151.21</th> | ||
| + | <th>474.651</th> | ||
| + | <th>99.334</th> | ||
| + | <th>358.843</th> | ||
| + | <th>97.329</th> | ||
| + | <th>115.674</th> | ||
| + | <th>97.686</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 4</th> | ||
| + | <th>88.651</th> | ||
| + | <th>553.489</th> | ||
| + | <th>158.106</th> | ||
| + | <th>471.047</th> | ||
| + | <th>107.222</th> | ||
| + | <th>391.116</th> | ||
| + | <th>102.558</th> | ||
| + | <th>132.724</th> | ||
| + | <th>104.901</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 1</th> | ||
| + | <th>94.201</th> | ||
| + | <th>799.161</th> | ||
| + | <th>176.469</th> | ||
| + | <th>396.183</th> | ||
| + | <th>105.65</th> | ||
| + | <th>452.456</th> | ||
| + | <th>105.862</th> | ||
| + | <th>129.379</th> | ||
| + | <th>100.254</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 2</th> | ||
| + | <th>97.455</th> | ||
| + | <th>750.414</th> | ||
| + | <th>168.71</th> | ||
| + | <th>425.555</th> | ||
| + | <th>106.286</th> | ||
| + | <th>462.598</th> | ||
| + | <th>109.024</th> | ||
| + | <th>125.311</th> | ||
| + | <th>101.914</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 3</th> | ||
| + | <th>95.836</th> | ||
| + | <th>795.354</th> | ||
| + | <th>174.674</th> | ||
| + | <th>405.952</th> | ||
| + | <th>103.704</th> | ||
| + | <th>444.673</th> | ||
| + | <th>106.365</th> | ||
| + | <th>128.606</th> | ||
| + | <th>105.761</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 4</th> | ||
| + | <th>100.733</th> | ||
| + | <th>804.913</th> | ||
| + | <th>203.631</th> | ||
| + | <th>370.775</th> | ||
| + | <th>117.227</th> | ||
| + | <th>506.476</th> | ||
| + | <th>113.511</th> | ||
| + | <th>125.021</th> | ||
| + | <th>105.18</th> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <table border="1"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Hour 6:</th> | ||
| + | <th>Neg. Control</th> | ||
| + | <th>Pos. Control</th> | ||
| + | <th>Device 1</th> | ||
| + | <th>Device 2</th> | ||
| + | <th>Device 3</th> | ||
| + | <th>Device 4</th> | ||
| + | <th>Device 5</th> | ||
| + | <th>Device 6</th> | ||
| + | <th>LB + Chlor (blank)</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 1</th> | ||
| + | <th>142.426</th> | ||
| + | <th>1579.487</th> | ||
| + | <th>407.651</th> | ||
| + | <th>2268.46</th> | ||
| + | <th>167.703</th> | ||
| + | <th>1014.837</th> | ||
| + | <th>144.06</th> | ||
| + | <th>365.966</th> | ||
| + | <th>130.779</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 2</th> | ||
| + | <th>143.238</th> | ||
| + | <th>1593.226</th> | ||
| + | <th>396.943</th> | ||
| + | <th>2328.22</th> | ||
| + | <th>174.862</th> | ||
| + | <th>1003.434</th> | ||
| + | <th>141.231</th> | ||
| + | <th>339.499</th> | ||
| + | <th>126.638</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 3</th> | ||
| + | <th>151.515</th> | ||
| + | <th>1699.317</th> | ||
| + | <th>396.669</th> | ||
| + | <th>2175.501</th> | ||
| + | <th>169.71</th> | ||
| + | <th>977.6</th> | ||
| + | <th>143.762</th> | ||
| + | <th>347.711</th> | ||
| + | <th>127.911</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 4</th> | ||
| + | <th>155.799</th> | ||
| + | <th>1783.066</th> | ||
| + | <th>406.205</th> | ||
| + | <th>2240.128</th> | ||
| + | <th>171.295</th> | ||
| + | <th>1044.087</th> | ||
| + | <th>154.138</th> | ||
| + | <th>340.887</th> | ||
| + | <th>127.237</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 1</th> | ||
| + | <th>150.46</th> | ||
| + | <th>2062.959</th> | ||
| + | <th>354.432</th> | ||
| + | <th>2090.23</th> | ||
| + | <th>165.189</th> | ||
| + | <th>1268.176</th> | ||
| + | <th>150.674</th> | ||
| + | <th>320.936</th> | ||
| + | <th>120.878</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 2</th> | ||
| + | <th>145.579</th> | ||
| + | <th>2023.17</th> | ||
| + | <th>366.367</th> | ||
| + | <th>2169.758</th> | ||
| + | <th>167.611</th> | ||
| + | <th>1203.787</th> | ||
| + | <th>144.458</th> | ||
| + | <th>322.957</th> | ||
| + | <th>127.103</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 3</th> | ||
| + | <th>146.553</th> | ||
| + | <th>2101.747</th> | ||
| + | <th>353.019</th> | ||
| + | <th>1926.808</th> | ||
| + | <th>183.724</th> | ||
| + | <th>1172.553</th> | ||
| + | <th>158.135</th> | ||
| + | <th>332.951</th> | ||
| + | <th>131.201</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 4</th> | ||
| + | <th>157.503</th> | ||
| + | <th>2071.459</th> | ||
| + | <th>316.402</th> | ||
| + | <th>1750.215</th> | ||
| + | <th>179.275</th> | ||
| + | <th>1253.449</th> | ||
| + | <th>155.625</th> | ||
| + | <th>327.385</th> | ||
| + | <th>137.828</th> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <br><br> | ||
| + | </div> | ||
| + | <h3>★ Abs600 Raw Readings：★ </h3> | ||
| + | <br> | ||
| + | <table border="1"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Hour 0:</th> | ||
| + | <th>Neg. Control</th> | ||
| + | <th>Pos. Control</th> | ||
| + | <th>Device 1</th> | ||
| + | <th>Device 2</th> | ||
| + | <th>Device 3</th> | ||
| + | <th>Device 4</th> | ||
| + | <th>Device 5</th> | ||
| + | <th>Device 6</th> | ||
| + | <th>LB + Chlor (blank)</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 1</th> | ||
| + | <th>0.1306</th> | ||
| + | <th>0.0884</th> | ||
| + | <th>0.0767</th> | ||
| + | <th>0.1068</th> | ||
| + | <th>0.1181</th> | ||
| + | <th>0.0753</th> | ||
| + | <th>0.1067</th> | ||
| + | <th>0.0866</th> | ||
| + | <th>0.0486</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 2</th> | ||
| + | <th>0.1272</th> | ||
| + | <th>0.0906</th> | ||
| + | <th>0.0737</th> | ||
| + | <th>0.1075</th> | ||
| + | <th>0.1184</th> | ||
| + | <th>0.0754</th> | ||
| + | <th>0.108</th> | ||
| + | <th>0.0896</th> | ||
| + | <th>0.0497</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 3</th> | ||
| + | <th>0.1246</th> | ||
| + | <th>0.09</th> | ||
| + | <th>0.0747</th> | ||
| + | <th>0.1119</th> | ||
| + | <th>0.1214</th> | ||
| + | <th>0.0731</th> | ||
| + | <th>0.1083</th> | ||
| + | <th>0.0879</th> | ||
| + | <th>0.0464</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 4</th> | ||
| + | <th>0.1225</th> | ||
| + | <th>0.0872</th> | ||
| + | <th>0.0743</th> | ||
| + | <th>0.1109</th> | ||
| + | <th>0.1247</th> | ||
| + | <th>0.0746</th> | ||
| + | <th>0.1079</th> | ||
| + | <th>0.0903</th> | ||
| + | <th>0.0476</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 1</th> | ||
| + | <th>0.1301</th> | ||
| + | <th>0.0907</th> | ||
| + | <th>0.0701</th> | ||
| + | <th>0.1109</th> | ||
| + | <th>0.1132</th> | ||
| + | <th>0.0813</th> | ||
| + | <th>0.1103</th> | ||
| + | <th>0.0909</th> | ||
| + | <th>0.0468</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 2</th> | ||
| + | <th>0.1327</th> | ||
| + | <th>0.0919</th> | ||
| + | <th>0.0685</th> | ||
| + | <th>0.1125</th> | ||
| + | <th>0.1145</th> | ||
| + | <th>0.0822</th> | ||
| + | <th>0.1081</th> | ||
| + | <th>0.0896</th> | ||
| + | <th>0.0472</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 3</th> | ||
| + | <th>0.1294</th> | ||
| + | <th>0.0952</th> | ||
| + | <th>0.0693</th> | ||
| + | <th>0.1151</th> | ||
| + | <th>0.1139</th> | ||
| + | <th>0.0809</th> | ||
| + | <th>0.1084</th> | ||
| + | <th>0.0898</th> | ||
| + | <th>0.05</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 4</th> | ||
| + | <th>0.1202</th> | ||
| + | <th>0.0941</th> | ||
| + | <th>0.0692</th> | ||
| + | <th>0.1105</th> | ||
| + | <th>0.1134</th> | ||
| + | <th>0.0805</th> | ||
| + | <th>0.1092</th> | ||
| + | <th>0.089</th> | ||
| + | <th>0.0472</th> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <table border="1"> | ||
| + | <thead> | ||
| + | <tr> | ||
| + | <th>Hour 6:</th> | ||
| + | <th>Neg. Control</th> | ||
| + | <th>Pos. Control</th> | ||
| + | <th>Device 1</th> | ||
| + | <th>Device 2</th> | ||
| + | <th>Device 3</th> | ||
| + | <th>Device 4</th> | ||
| + | <th>Device 5</th> | ||
| + | <th>Device 6</th> | ||
| + | <th>LB + Chlor (blank)</th> | ||
| + | </tr> | ||
| + | </thead> | ||
| + | <tbody> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 1</th> | ||
| + | <th>0.9386</th> | ||
| + | <th>0.7564</th> | ||
| + | <th>0.1978</th> | ||
| + | <th>0.911</th> | ||
| + | <th>0.7963</th> | ||
| + | <th>0.2566</th> | ||
| + | <th>0.8131</th> | ||
| + | <th>0.6363</th> | ||
| + | <th>0.0444</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 2</th> | ||
| + | <th>0.9413</th> | ||
| + | <th>0.7528</th> | ||
| + | <th>0.1974</th> | ||
| + | <th>0.9012</th> | ||
| + | <th>0.8211</th> | ||
| + | <th>0.2547</th> | ||
| + | <th>0.7951</th> | ||
| + | <th>0.5996</th> | ||
| + | <th>0.0438</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 3</th> | ||
| + | <th>0.9745</th> | ||
| + | <th>0.7748</th> | ||
| + | <th>0.1951</th> | ||
| + | <th>0.8642</th> | ||
| + | <th>0.81</th> | ||
| + | <th>0.2536</th> | ||
| + | <th>0.7994</th> | ||
| + | <th>0.6044</th> | ||
| + | <th>0.0458</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 4</th> | ||
| + | <th>0.9932</th> | ||
| + | <th>0.7975</th> | ||
| + | <th>0.2009</th> | ||
| + | <th>0.8969</th> | ||
| + | <th>0.8021</th> | ||
| + | <th>0.2622</th> | ||
| + | <th>0.8266</th> | ||
| + | <th>0.6092</th> | ||
| + | <th>0.0438</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 1</th> | ||
| + | <th>0.8246</th> | ||
| + | <th>0.78</th> | ||
| + | <th>0.1031</th> | ||
| + | <th>0.8625</th> | ||
| + | <th>0.8193</th> | ||
| + | <th>0.2834</th> | ||
| + | <th>0.7633</th> | ||
| + | <th>0.5251</th> | ||
| + | <th>0.0457</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 2</th> | ||
| + | <th>0.8327</th> | ||
| + | <th>0.7665</th> | ||
| + | <th>0.1031</th> | ||
| + | <th>0.9018</th> | ||
| + | <th>0.8211</th> | ||
| + | <th>0.2781</th> | ||
| + | <th>0.7394</th> | ||
| + | <th>0.5374</th> | ||
| + | <th>0.0462</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 3</th> | ||
| + | <th>0.8062</th> | ||
| + | <th>0.7927</th> | ||
| + | <th>0.1005</th> | ||
| + | <th>0.829</th> | ||
| + | <th>0.8082</th> | ||
| + | <th>0.276</th> | ||
| + | <th>0.7689</th> | ||
| + | <th>0.5668</th> | ||
| + | <th>0.0458</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 4</th> | ||
| + | <th>0.8262</th> | ||
| + | <th>0.7805</th> | ||
| + | <th>0.1005</th> | ||
| + | <th>0.8787</th> | ||
| + | <th>0.8156</th> | ||
| + | <th>0.2928</th> | ||
| + | <th>0.7604</th> | ||
| + | <th>0.5332</th> | ||
| + | <th>0.0477</th> | ||
| + | </tr> | ||
| + | </tbody> | ||
| + | </table> | ||
| + | <br><br> | ||
| + | <h3>★ uM Fluorescein / OD ★ </h3> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <th>Hour 0:</th> | ||
| + | <th>Neg. Control</th> | ||
| + | <th>Pos. Control</th> | ||
| + | <th>Device 1</th> | ||
| + | <th>Device 2</th> | ||
| + | <th>Device 3</th> | ||
| + | <th>Device 4</th> | ||
| + | <th>Device 5</th> | ||
| + | <th>Device 6</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>olony 1, Replicate 1</th> | ||
| + | <th>-0.002</th> | ||
| + | <th>0.872</th> | ||
| + | <th>0.208</th> | ||
| + | <th>0.508</th> | ||
| + | <th>0.008</th> | ||
| + | <th>0.871</th> | ||
| + | <th>0.014</th> | ||
| + | <th>0.046</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 2</th> | ||
| + | <th>-0.011</th> | ||
| + | <th>0.866</th> | ||
| + | <th>0.201</th> | ||
| + | <th>0.501</th> | ||
| + | <th>0.006</th> | ||
| + | <th>0.833</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.042</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 3</th> | ||
| + | <th>-0.009</th> | ||
| + | <th>0.775</th> | ||
| + | <th>0.159</th> | ||
| + | <th>0.482</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.820</th> | ||
| + | <th>0.000</th> | ||
| + | <th>0.036</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 4</th> | ||
| + | <th>-0.018</th> | ||
| + | <th>0.950</th> | ||
| + | <th>0.167</th> | ||
| + | <th>0.485</th> | ||
| + | <th>0.003</th> | ||
| + | <th>0.889</th> | ||
| + | <th>-0.003</th> | ||
| + | <th>0.055</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 1</th> | ||
| + | <th>-0.006</th> | ||
| + | <th>1.334</th> | ||
| + | <th>0.274</th> | ||
| + | <th>0.387</th> | ||
| + | <th>0.007</th> | ||
| + | <th>0.856</th> | ||
| + | <th>0.007</th> | ||
| + | <th>0.055</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 2</th> | ||
| + | <th>-0.004</th> | ||
| + | <th>1.216</th> | ||
| + | <th>0.263</th> | ||
| + | <th>0.415</th> | ||
| + | <th>0.005</th> | ||
| + | <th>0.864</th> | ||
| + | <th>0.010</th> | ||
| + | <th>0.046</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 3</th> | ||
| + | <th>-0.010</th> | ||
| + | <th>1.279</th> | ||
| + | <th>0.299</th> | ||
| + | <th>0.387</th> | ||
| + | <th>-0.003</th> | ||
| + | <th>0.919</th> | ||
| + | <th>0.001</th> | ||
| + | <th>0.048</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 4</th> | ||
| + | <th>-0.005</th> | ||
| + | <th>1.251</th> | ||
| + | <th>0.375</th> | ||
| + | <th>0.352</th> | ||
| + | <th>0.015</th> | ||
| + | <th>1.010</th> | ||
| + | <th>0.011</th> | ||
| + | <th>0.040</th> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <th>Hour 6:</th> | ||
| + | <th>Neg. Control</th> | ||
| + | <th>Pos. Control</th> | ||
| + | <th>Device 1</th> | ||
| + | <th>Device 2</th> | ||
| + | <th>Device 3</th> | ||
| + | <th>Device 4</th> | ||
| + | <th>Device 5</th> | ||
| + | <th>Device 6</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 1</th> | ||
| + | <th>0.001</th> | ||
| + | <th>0.171</th> | ||
| + | <th>0.151</th> | ||
| + | <th>0.207</th> | ||
| + | <th>0.004</th> | ||
| + | <th>0.349</th> | ||
| + | <th>0.001</th> | ||
| + | <th>0.033</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 2</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.173</th> | ||
| + | <th>0.148</th> | ||
| + | <th>0.215</th> | ||
| + | <th>0.005</th> | ||
| + | <th>0.348</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.032</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 3</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.181</th> | ||
| + | <th>0.151</th> | ||
| + | <th>0.210</th> | ||
| + | <th>0.005</th> | ||
| + | <th>0.343</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.033</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 4</th> | ||
| + | <th>0.003</th> | ||
| + | <th>0.184</th> | ||
| + | <th>0.149</th> | ||
| + | <th>0.208</th> | ||
| + | <th>0.005</th> | ||
| + | <th>0.352</th> | ||
| + | <th>0.003</th> | ||
| + | <th>0.032</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 1</th> | ||
| + | <th>0.003</th> | ||
| + | <th>0.222</th> | ||
| + | <th>0.341</th> | ||
| + | <th>0.202</th> | ||
| + | <th>0.005</th> | ||
| + | <th>0.405</th> | ||
| + | <th>0.003</th> | ||
| + | <th>0.035</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 2</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.221</th> | ||
| + | <th>0.352</th> | ||
| + | <th>0.200</th> | ||
| + | <th>0.004</th> | ||
| + | <th>0.389</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.033</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 3</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.221</th> | ||
| + | <th>0.340</th> | ||
| + | <th>0.192</th> | ||
| + | <th>0.006</th> | ||
| + | <th>0.379</th> | ||
| + | <th>0.003</th> | ||
| + | <th>0.032</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 4</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.221</th> | ||
| + | <th>0.283</th> | ||
| + | <th>0.163</th> | ||
| + | <th>0.005</th> | ||
| + | <th>0.382</th> | ||
| + | <th>0.002</th> | ||
| + | <th>0.033</th> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br><br> | ||
| + | <h3>★ Net Fluorescein a.u. ★ </h3> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <th>Hour 0:</th> | ||
| + | <th>Neg. Control</th> | ||
| + | <th>Pos. Control</th> | ||
| + | <th>Device 1</th> | ||
| + | <th>Device 2</th> | ||
| + | <th>Device 3</th> | ||
| + | <th>Device 4</th> | ||
| + | <th>Device 5</th> | ||
| + | <th>Device 6</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 1</th> | ||
| + | <th>-1.67</th> | ||
| + | <th>413.95</th> | ||
| + | <th>69.89</th> | ||
| + | <th>352.65</th> | ||
| + | <th>6.97</th> | ||
| + | <th>277.44</th> | ||
| + | <th>9.77</th> | ||
| + | <th>20.72</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 2</th> | ||
| + | <th>-10.03</th> | ||
| + | <th>422.59</th> | ||
| + | <th>57.66</th> | ||
| + | <th>345.74</th> | ||
| + | <th>5.18</th> | ||
| + | <th>255.40</th> | ||
| + | <th>1.59</th> | ||
| + | <th>19.87</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 3</th> | ||
| + | <th>-8.02</th> | ||
| + | <th>402.92</th> | ||
| + | <th>53.52</th> | ||
| + | <th>376.97</th> | ||
| + | <th>1.65</th> | ||
| + | <th>261.16</th> | ||
| + | <th>-0.36</th> | ||
| + | <th>17.99</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 4</th> | ||
| + | <th>-16.25</th> | ||
| + | <th> 448.59</th> | ||
| + | <th> 53.21 </th> | ||
| + | <th>366.15 </th> | ||
| + | <th>2.32 </th> | ||
| + | <th>286.22 </th> | ||
| + | <th>-2.34 </th> | ||
| + | <th>27.82</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 1 </th> | ||
| + | <th>-6.05</th> | ||
| + | <th> 698.91 </th> | ||
| + | <th>76.22 </th> | ||
| + | <th>295.93 </th> | ||
| + | <th>5.40</th> | ||
| + | <th> 352.20 </th> | ||
| + | <th>5.61</th> | ||
| + | <th>29.13</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 2 </th> | ||
| + | <th>-4.46 </th> | ||
| + | <th>648.50</th> | ||
| + | <th> 66.80 </th> | ||
| + | <th>323.64 </th> | ||
| + | <th>4.37</th> | ||
| + | <th> 360.68</th> | ||
| + | <th> 7.11 </th> | ||
| + | <th>23.40</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 3</th> | ||
| + | <th> -9.93 </th> | ||
| + | <th>689.59</th> | ||
| + | <th> 68.91 </th> | ||
| + | <th>300.19</th> | ||
| + | <th> -2.06 </th> | ||
| + | <th>338.91 </th> | ||
| + | <th>0.60 </th> | ||
| + | <th>22.85</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 4 </th> | ||
| + | <th>-4.45 </th> | ||
| + | <th>699.73 </th> | ||
| + | <th>98.45 </th> | ||
| + | <th>265.60</th> | ||
| + | <th> 12.05 </th> | ||
| + | <th>401.30</th> | ||
| + | <th> 8.33</th> | ||
| + | <th> 19.84</th> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <th>Hour 6: </th> | ||
| + | <th>Neg. Control </th> | ||
| + | <th>Pos. Control </th> | ||
| + | <th>Device 1</th> | ||
| + | <th> Device 2</th> | ||
| + | <th> Device 3</th> | ||
| + | <th> Device 4 </th> | ||
| + | <th>Device 5</th> | ||
| + | <th> Device 6</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 1 </th> | ||
| + | <th>11.65 </th> | ||
| + | <th>1448.71 </th> | ||
| + | <th>276.87</th> | ||
| + | <th> 2137.68 </th> | ||
| + | <th>36.92 </th> | ||
| + | <th>884.06 </th> | ||
| + | <th>13.28 </th> | ||
| + | <th>235.19</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 2 </th> | ||
| + | <th>16.60 </th> | ||
| + | <th>1466.59 </th> | ||
| + | <th>270.31 </th> | ||
| + | <th>2201.58 </th> | ||
| + | <th>48.22 </th> | ||
| + | <th>876.80</th> | ||
| + | <th> 14.59 </th> | ||
| + | <th>212.86</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 3</th> | ||
| + | <th> 23.60 </th> | ||
| + | <th>1571.41 </th> | ||
| + | <th>268.76 </th> | ||
| + | <th>2047.59 </th> | ||
| + | <th>41.80 </th> | ||
| + | <th>849.69 </th> | ||
| + | <th>15.85 </th> | ||
| + | <th>219.80</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 4 </th> | ||
| + | <th>28.56 </th> | ||
| + | <th>1655.83 </th> | ||
| + | <th>278.97 </th> | ||
| + | <th>2112.89 </th> | ||
| + | <th>44.06 </th> | ||
| + | <th>916.85 </th> | ||
| + | <th>26.90 </th> | ||
| + | <th>213.65</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 1 </th> | ||
| + | <th>29.58 </th> | ||
| + | <th>1942.08 </th> | ||
| + | <th>233.55 </th> | ||
| + | <th>1969.35 </th> | ||
| + | <th>44.31 </th> | ||
| + | <th>1147.30 </th> | ||
| + | <th>29.80 </th> | ||
| + | <th>200.06</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 2 </th> | ||
| + | <th>18.48 </th> | ||
| + | <th>1896.07 </th> | ||
| + | <th>239.26 </th> | ||
| + | <th>2042.66 </th> | ||
| + | <th>40.51 </th> | ||
| + | <th>1076.68 </th> | ||
| + | <th>17.36</th> | ||
| + | <th> 195.85</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 3 </th> | ||
| + | <th>15.35 </th> | ||
| + | <th>1970.55 </th> | ||
| + | <th>221.82 </th> | ||
| + | <th>1795.61 </th> | ||
| + | <th>52.52 </th> | ||
| + | <th>1041.35 </th> | ||
| + | <th>26.93 </th> | ||
| + | <th>201.75</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 4 </th> | ||
| + | <th>19.68 </th> | ||
| + | <th>1933.63 </th> | ||
| + | <th>178.57 </th> | ||
| + | <th>1612.39 </th> | ||
| + | <th>41.45 </th> | ||
| + | <th>1115.62 </th> | ||
| + | <th>17.80 </th> | ||
| + | <th>189.56</th> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <br><br> | ||
| + | <h3>★ Net Abs 600 ★ </h3> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <th>Hour 0:</th> | ||
| + | <th>Neg. Control</th> | ||
| + | <th>Pos. Control</th> | ||
| + | <th>Device 1</th> | ||
| + | <th>Device 2</th> | ||
| + | <th>Device 3</th> | ||
| + | <th>Device 4</th> | ||
| + | <th>Device 5</th> | ||
| + | <th>Device 6</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 1 </th> | ||
| + | <th>0.082 </th> | ||
| + | <th>0.040 </th> | ||
| + | <th>0.028 </th> | ||
| + | <th>0.058 </th> | ||
| + | <th>0.070 </th> | ||
| + | <th>0.027 </th> | ||
| + | <th>0.058 </th> | ||
| + | <th>0.038</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 2 </th> | ||
| + | <th>0.078 </th> | ||
| + | <th>0.041 </th> | ||
| + | <th>0.024 </th> | ||
| + | <th>0.058 </th> | ||
| + | <th>0.069 </th> | ||
| + | <th>0.026 </th> | ||
| + | <th>0.058 </th> | ||
| + | <th>0.040</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 3 </th> | ||
| + | <th>0.078 </th> | ||
| + | <th>0.044 </th> | ||
| + | <th>0.028 </th> | ||
| + | <th>0.066 </th> | ||
| + | <th>0.075 </th> | ||
| + | <th>0.027 </th> | ||
| + | <th>0.062</th> | ||
| + | <th> 0.042</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 4 </th> | ||
| + | <th>0.075 </th> | ||
| + | <th>0.040 </th> | ||
| + | <th>0.027 </th> | ||
| + | <th>0.063 </th> | ||
| + | <th>0.077 </th> | ||
| + | <th>0.027 </th> | ||
| + | <th>0.060 </th> | ||
| + | <th>0.043</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 1 </th> | ||
| + | <th>0.083 </th> | ||
| + | <th>0.044</th> | ||
| + | <th> 0.023 </th> | ||
| + | <th>0.064 </th> | ||
| + | <th>0.066 </th> | ||
| + | <th>0.035</th> | ||
| + | <th> 0.064 </th> | ||
| + | <th>0.044</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 2</th> | ||
| + | <th> 0.086 </th> | ||
| + | <th>0.045 </th> | ||
| + | <th>0.021 </th> | ||
| + | <th>0.065 </th> | ||
| + | <th>0.067 </th> | ||
| + | <th>0.035 </th> | ||
| + | <th>0.061</th> | ||
| + | <th> 0.042</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 3 </th> | ||
| + | <th>0.079</th> | ||
| + | <th> 0.045 </th> | ||
| + | <th>0.019</th> | ||
| + | <th> 0.065 </th> | ||
| + | <th>0.064 </th> | ||
| + | <th>0.031 </th> | ||
| + | <th>0.058 </th> | ||
| + | <th>0.040</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 4 </th> | ||
| + | <th>0.073 </th> | ||
| + | <th>0.047 </th> | ||
| + | <th>0.022 </th> | ||
| + | <th>0.063 </th> | ||
| + | <th>0.066 </th> | ||
| + | <th>0.033 </th> | ||
| + | <th>0.062 </th> | ||
| + | <th>0.042</th> | ||
| + | </tr> | ||
| + | </table> | ||
| + | <table> | ||
| + | <tr> | ||
| + | <th>Hour 6: </th> | ||
| + | <th>Neg. Control </th> | ||
| + | <th>Pos. Control </th> | ||
| + | <th>Device 1</th> | ||
| + | <th> Device 2</th> | ||
| + | <th> Device 3</th> | ||
| + | <th> Device 4 </th> | ||
| + | <th>Device 5</th> | ||
| + | <th> Device 6</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 1</th> | ||
| + | <th> 0.894</th> | ||
| + | <th> 0.712 </th> | ||
| + | <th>0.153 </th> | ||
| + | <th>0.867 </th> | ||
| + | <th>0.752 </th> | ||
| + | <th>0.212 </th> | ||
| + | <th>0.769 </th> | ||
| + | <th>0.592</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 2 </th> | ||
| + | <th>0.898 </th> | ||
| + | <th>0.709 </th> | ||
| + | <th>0.154 </th> | ||
| + | <th>0.8576</th> | ||
| + | <th> 0.777 </th> | ||
| + | <th>0.211 </th> | ||
| + | <th>0.751</th> | ||
| + | <th> 0.55</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 3 </th> | ||
| + | <th>0.929</th> | ||
| + | <th> 0.729 </th> | ||
| + | <th>0.149 </th> | ||
| + | <th>0.818 </th> | ||
| + | <th>0.764</th> | ||
| + | <th> 0.208 </th> | ||
| + | <th>0.754</th> | ||
| + | <th> 0.559</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 1, Replicate 4 </th> | ||
| + | <th>0.949 </th> | ||
| + | <th>0.754</th> | ||
| + | <th> 0.157 </th> | ||
| + | <th>0.853 </th> | ||
| + | <th>0.758</th> | ||
| + | <th> 0.218 </th> | ||
| + | <th>0.783 </th> | ||
| + | <th>0.565</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 1 </th> | ||
| + | <th>0.779 </th> | ||
| + | <th>0.734 </th> | ||
| + | <th>0.057 </th> | ||
| + | <th>0.817 </th> | ||
| + | <th>0.774 </th> | ||
| + | <th>0.238 </th> | ||
| + | <th>0.718 </th> | ||
| + | <th>0.479</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 2</th> | ||
| + | <th> 0.787 </th> | ||
| + | <th>0.720 </th> | ||
| + | <th>0.057</th> | ||
| + | <th> 0.856 </th> | ||
| + | <th>0.775 </th> | ||
| + | <th>0.232 </th> | ||
| + | <th>0.693</th> | ||
| + | <th> 0.491</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 3 </th> | ||
| + | <th>0.760</th> | ||
| + | <th> 0.747 </th> | ||
| + | <th>0.055</th> | ||
| + | <th> 0.783 </th> | ||
| + | <th>0.762</th> | ||
| + | <th> 0.230 </th> | ||
| + | <th>0.723 </th> | ||
| + | <th>0.521</th> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <th>Colony 2, Replicate 4 </th> | ||
| + | <th>0.779 </th> | ||
| + | <th>0.733 </th> | ||
| + | <th>0.053 </th> | ||
| + | <th>0.831 </th> | ||
| + | <th>0.768 </th> | ||
| + | <th>0.245 </th> | ||
| + | <th>0.713 </th> | ||
| + | <th>0.486</th> | ||
| + | </tr> | ||
| + | </table>--> | ||
| + | <div class="column full_size judges-will-not-evaluate middle"> | ||
| + | <h1>CFU Protocol</h1> | ||
| + | <h3>For this part of inter lab study, we followed the following protocol:</h3> | ||
| + | <p>1.Pick two colonies from positive control device and negative control device, incubate overnight. | ||
| + | <br> | ||
| + | 2.Prepare Starting Samples: Dilute the overnight cultures 1:8, measurement then dilute further to OD600nm = 0.1 | ||
| + | <br> | ||
| + | Calculation: | ||
| + | <br> | ||
| + | Use (C1)(V1) = (C2)(V2) to calculate your dilutions | ||
| + | C1 is your starting OD600   C2 is your target OD600 of 0.1 | ||
| + | <br> | ||
| + | V1 is the unknown volume in μL   V2 is the final volume of 1000 μL | ||
| + | <br> | ||
| + | 3.Check and make sure OD600 = 0.1 | ||
| + | <br> | ||
| + | 4.Dilute | ||
| + | <img src="https://static.igem.org/mediawiki/2018/e/ee/T--ZJUT-China--Dilute.png" alt="Dilute"> | ||
| + | <br> | ||
| + | 5.Incubate at 37 degree Celsius overnight for 18-20 hrs. Then count colony number. | ||
| + | <br> | ||
| + | <img src="https://static.igem.org/mediawiki/2018/4/4c/T--ZJUT-China--colony_number.png" width="900px"alt="colony_number"> | ||
| + | </p> | ||
| + | </div> | ||
| + | |||
| + | </p> | ||
| + | </div> | ||
| + | </div> | ||
</div> | </div> | ||
</div> | </div> | ||
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| − | + | <h3>Contact</h3> | |
| − | + | <br><br> | |
| − | + | <p>College of Bioengineering | |
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| − | + | <br><br>Zhejiang University of Technology | |
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| − | + | <br><br>310000, Hangzhou | |
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Revision as of 15:38, 13 September 2018
Team:ZJUT-China
Interlab
Our team participated in the Fifth International InterLaboratory Measurement Study this year. The interlab study aims to solve the problem of reliability and repeatability in synthetic biology study. This year we were asked to measure green fluorescent protein, which is used as a measurement marker. We followed the experiment protocol strictly to make sure our data is valid. After two weeks lab work, we got the data we need after several attempts.
Calibration Protocol
Three calibration measurement were done by the calibration protocol, including an OD reference point at 600nm, one particle standard curve and one fluorescent standard curve.
(Plate Reader: Molecular Device, Spectramax M5)
★ OD600 reference point ★
| LUDOX CL-X | H2O | |
|---|---|---|
| Replicate 1 | 0.066 | 0.0285 |
| Replicate 2 | 0.059 | 0.029 |
| Replicate 3 | 0.068 | 0.026 |
| Replicate 4 | 0.064 | 0.028 |
| Arith. Mean | 0.064 | 0.028 |
| Corrected Abs600 | 0.036 | |
| Reference OD600 | OD600 | |
| OD600/Abs600 | 1.728 |
Form1.OD reference point
Fig1.Particle standard curve
Fig2.Particle standard curve (log_scale)
Fig3.Fluorescein standard curve
Fig4.Fluorescein standard curve (log_scale)
Cell measurement
Transformation
Transform Escherichia coli DH5α with these following plasmids:
| Device | Part Number | Location |
|---|---|---|
| Positive control | BBa_R0040 | Well 2D |
| Negative control | Ba_I20270 | Well 2B |
| Test Device 1 | BBa_J364000 | Well 2F |
| Test Device 2 | BBa_J364001 | Well 2H |
| Test Device 3 | BBa_J364002 | Well 2J |
| Test Device 4 | BBa_J364007 | Well 2L |
| Test Device 5 | BBa_J364008 | Well 2N |
| Test Device 6 | BBa_J364009 | Well 2P |
Form2.Devices (from Distribution Kit, all in pSB1C3 backbone)
The transformation used E.coli DH5α competent cells bought from Tsingke Biological Technology company, and follow the steps in http://parts.igem.org/Help:2017_DNA_Distribution to use the DNA in the Distribution Kit.
Measurement
For this part of inter lab study, we followed the following protocols:
1.Grown 8 devices in incubator for 12 hrs at 37 ℃
2.Pick 2 colonies from each of plate and inocubate them on 10mL LB medium with Chloramphenicol. Grow the cells for 16-20hrs at 37°C and 180rpm.
3.Measure OD600nm of the overnight cultures and record the data, then dilute to target OD600nm = 0.02 in the falcon tubes.
4.Measure the OD600nm and Fl under the same condition as standard curve measurement and use the same 96 wells plates.
Fig5. plate lay out
★ Fluorescence Raw Readings：★
★ Abs600 Raw Readings：★
★ uM Fluorescein / OD ★
★ Net Fluorescein a.u. ★
★ Net Abs 600 ★
Form3-6. Result of Raw Plate Reader Measurements
CFU Protocol
For this part of inter lab study, we followed the following protocol:
1.Pick two colonies from positive control device and negative control device, incubate overnight.
2.Prepare Starting Samples: Dilute the overnight cultures 1:8, measurement then dilute further to OD600nm = 0.1
Calculation:
Use (C1)(V1) = (C2)(V2) to calculate your dilutions
C1 is your starting OD600 C2 is your target OD600 of 0.1
V1 is the unknown volume in μL V2 is the final volume of 1000 μL
3.Check and make sure OD600 = 0.1
4.Dilute
5.Incubate at 37 degree Celsius overnight for 18-20 hrs. Then count colony number.
Copyright © 2018 ZJUT-China